백서 두개골 결손에서 rhBMP-2와 다양한 carrier의 골재생 유도효과
Effects of rhBMP-2 with various carriers on bone regeneration in rat calvarial defec
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Purpose: Bone morphogenetic protein (BMP) is a potent differentiating agent for cells of the osteoblastic lineage. It has been used in the oral cavity under a variety of indications and with different carriers. However, the optimal carrier for each indication is not known. This study evaluated the bone regenerative effect of rhBMP-2 delivered with different carrier systems. Materials and Methods: 8mm critical-sized rat calvarial defects were used in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each. Two groups were controls that had no treatment and absorbable collagen membrane only. 4 groups were experimentals that contained rhBMP-2 only and applied with absorbable collagen sponge(Collatape?), MBCP?, Bio-Oss? each. The histological and histometric parameters were used to evaluate the defects after 2- or 8-week healing period. The shape and total augmented area were stable in all groups over the healing time. Results: New bone formation was significantly greater in the rhBMP-2 with carrier group than control group. rhBMP-2/ACS was the highest in bone density but gained less new bone area than rhBMP-2/MBCP? and rhBMP-2/Bio-Oss?. The bone density after 8 weeks was greater than that after 2 weeks in all groups. However, rhBMP-2 alone failed to show the statistically significant difference in new bone area and bone density compared to control group. Also MBCP? and Bio-Oss? particles remained after 8 weeks healing period.Conclusion: These results suggest that rhBMP-2 with carrier system is an excellent inductive agent for bone formation and we can use it as the predictable bone tissue engieering technique. Future study will likely focus on the kinetics of BMP release and development of carriers that is ideal for it. (J Korean Acad Periodontol 2008;38:125-134)
Abstract
Purpose: Bone morphogenetic protein (BMP) is a potent differentiating agent for cells of the osteoblastic lineage. It has been used in the oral cavity under a variety of indications and with different carriers. However, the optimal carrier for each indication is not known. This study evaluated the bone regenerative effect of rhBMP-2 delivered with different carrier systems. Materials and Methods: 8mm critical-sized rat calvarial defects were used in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each. Two groups were controls that had no treatment and absorbable collagen membrane only. 4 groups were experimentals that contained rhBMP-2 only and applied with absorbable collagen sponge(Collatape?), MBCP?, Bio-Oss? each. The histological and histometric parameters were used to evaluate the defects after 2- or 8-week healing period. The shape and total augmented area were stable in all groups over the healing time. Results: New bone formation was significantly greater in the rhBMP-2 with carrier group than control group. rhBMP-2/ACS was the highest in bone density but gained less new bone area than rhBMP-2/MBCP? and rhBMP-2/Bio-Oss?. The bone density after 8 weeks was greater than that after 2 weeks in all groups. However, rhBMP-2 alone failed to show the statistically significant difference in new bone area and bone density compared to control group. Also MBCP? and Bio-Oss? particles remained after 8 weeks healing period.Conclusion: These results suggest that rhBMP-2 with carrier system is an excellent inductive agent for bone formation and we can use it as the predictable bone tissue engieering technique. Future study will likely focus on the kinetics of BMP release and development of carriers that is ideal for it. (J Korean Acad Periodontol 2008;38:125-134)
참고문헌 (36)
- Ahn SH, "Effect of Recombinant Human Bone Morphogenetic Protein- 4 with Carriers in Rat Calvarial Defects", J Periodontol, 74, pp.787~797 (2003)
- Alam MI, "Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitute", J Biomed Mater Res, 54, pp.129~138 (2001)
- Alam MI, "Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2", Biomaterials, 22, pp.1643~1651 (2001)
- Boden SD, "Differential effects and glucocorticoid potentiation of bone morphogenetic protein action during rat osteoblast differentiation in vitro", Endocrinology, 137, pp.3401~3407 (1996)
- Bosch C, "Importance of the critical-size bone defect in testing bone-regenerating materials", J Craniofac Surg, 9, pp.310~316 (1998)
- Choi SH, "Effect of recombinant human bone morphogenetic protein-2", absorbable collagen sponge (rhBMP-2, J Periodontol, pp.2002, 73[DOI]
- Cordaro, "Clinical results of alveolar ridge augmentation with mandibular block bone grafts in partially edentulous patients prior to implant placement", Clin Oral Implants Res, 13, pp.103~111 (2002)
- Dragoo MR, "A clinical and hitological evaluation of autogenous iliac bone trafts in humans. II External root resorption", J Periodontol, 44, pp.614~625 (1973)
- Goldbefg VM, "Natural history of autografts and allografts", Clin Orhop, 225, pp.7~16 (1987)
- Hollinger JO, "Carriers for bone morphogenetic proteins", Biometerials, 17, pp.187~194 (1996)
- Han DK, "Effect of a Fibrin-fibronectin sealing system as a carrier for recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects", J Periodontol, 76, pp.2216~2222 (2005)
- Hong SJ, "The Effect of a Fibrin-Fibronectin", ß-Tricalcium Phosphate, Biomaterials, pp.2006, 27[DOI]
- Hyun SJ, "The effect of recombinant human bone morphogenetic protein-2, 4 and 7 on bone formation in rat calvarial defects", J Periodontol, 76, pp.1667~1674 (2005)
- Jung UW, "The Effect of varying the Particle Size of Beta Tricalcium Phosphate carrier of Recombinant Human Bone Morphogenetic Protein-4 on Bone Formation in Rat Calvarial Defects", J Periodontol, 77, pp.765~772 (2006)
- Karring T, "Regenerative periodontal therapy", Clin Perio Imp Dent, pp.599~602 (2002)
- Kim CS, "The effect of recombinant human bone morphogenetic protein-4 on the osteoblastic differentiation of mouse calvarial cells affected by Porphyromonas gingivalis", J Periodontol, 73, pp.1126~1132 (2002)
- Kim CS, "Ectopic bone formation associated with recombinant human bone morphogenetic protein-2 using absorbable collagen sponge and beta tricalcium phosphate as carriers", Biomaterials, 19, pp.1~7 (2004)
- Lee YM, "The bone regenerative effect of platelet-derived growth factor-BB delivered with a chitosan", tricalcium phosphate sponge carrier, 2000, pp.71, 418~424
- Mallonig JT, "Autogenous and allogenic bone grafts in peiodontal therapy", Crit Rev Oral Biol Med, 3, pp.333~352 (1992)
- Mao T, "An experimental study on rhBMP-2 composite bone substitute for repairing craniomaxillary bone defects", Clin J Dent Res, 1, pp.21~25 (1998)
- Marden LJ, "Recombinant human bone morphogenetic protein-2 is superior to demineralized bone matrix in repairing craniotomy defects in rats", J Biomed Mater Res, 28, pp.1127~1138 (1994)
- Misch CE, "Bone-grafting materials in implant dentistry", Implant Dent, 2, pp.158~167 (1993)
- Mizutani H, "The nature of bone morphogenetic protein(BMP) fractions derived from bovine bone matrix gelatin", Clin Orhop, 171, pp.213~223 (1982)
- Okura K, "Healing of segmental bone defects in rats induced by a β TCP-MCPM cement combine with rhBMP-2", J Biomed Mater Res, 44, pp.168~175 (1999)
- Pang EK, "Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in rat calvarial defects", J Periodontol, 75, pp.1364~1370 (2004)
- Sampath TK, "Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenic protein and stimulates osteoblast proliferation and differentiation in vitro", J Biol Chem, 267, pp.20352~20362 (1992)
- Sandor G, "Successful mandibular reconstruction using a BMP bioimplant", J Craniofac Surg, 12(2), pp.119~127 (2001)
- Schwartz-Arad D, "Surgical success of intraoral autogenous block onlay bone grafting for alveolar ridge augmentation", Implant Dent, 14, pp.131~138 (2005)
- Sigurdsson TJ, "Periodontal repair in dogs: Evaluation of rhBMP-2 carriers", Int J Perio Rest Dent, 16, pp.524~537 (1996)
- Takagi K, "The reaction of the dura to bone morphogenetic protein(BMP) in repair of skull defects", Ann Surg, 196, pp.100~109 (1982)
- Uludag H, "Implantation of recombinant human bone morphogenetic proteins with biomaterial carriers; A correlation between protein pharmacokinetics and osteoinduction in the rat ectopic model", J Biomed Mater Res, 50, pp.227~238 (2000)
- Urist MR, "Bone formation by autoinduction", Science, 150, pp.893~899 (1965)
- Urist MR, "bovine low molecular weight bone morphogenetic protein (BMP) fraction", Clin Orthop, 162, pp.219~232 (1982)
- Wikesjoὂ UM, "Periodontal repair in dogs: Effect of BMP-2 concentration on regeneration of alveolar bone and periodontal attachment", J Clin Periodontol, 26, pp.392~400 (1999)
- Winn SR, "Carrier systems for bone morphogenetic proteins", Clin Orhop, 367(suppl), pp.95s~106s (1999)
- Yamaguchi A, "Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3-G2", PA6, 1996, pp.220, 336~371
