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백서두개골 결손부에서 Escherichia coli 발현 시스템으로 생산된 rhBMP-2의 골형성 효과

Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

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초록

Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration. (J Korean Acad Periodontol 2009;39:77-86)

Abstract

Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration. (J Korean Acad Periodontol 2009;39:77-86)

참고문헌 (35)

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관련 논문AI추천

백서 두개골 결손에서 rhBMP-2와 다양한 carrier의 골재생 유도효과강은정, 김종관, 김지선, 김창성, 엄태관, 이서경, 조규성, 채중규, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호E. coli 발현 시스템에 의해 생산된 recombinant human bone morphogenetic protein-2의 정제와 생물학적 활성김수홍, 문금옥, 윤정호, 장경립, 조규성, 최경희대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제1호Effects of Escherichia Coli-derived Recombinant Human Bone Morphogenetic Protein-2 Loaded Porous Hydroxyaptite-based Ceramics on Calvarial Defect in Rabbits김신영, 김용건, 서승준, 이영균, 임재홍대한골대사학회 · 대한골대사학회지2017 · 제24권 제1호단백질 전달 영역 융합-Bone Morphogenetic Protein-2가 백서 두개골 결손부에서 골 조직 재생에 미치는 효과김종관, 김창성, 엄유정, 조규성, 채중규, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호Effect of MBCP block as carrier of rhBMP-2 in combination with ePTFE membrane on bone formation in rat calvarial defects김창성, 신철우, 윤정호, 정성원, 조규성, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue김준일, 김창성, 윤정호, 정성원, 조규성, 채경준대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호

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