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사람 치주인대섬유모세포에서 MMP-1, TIMP-1,2, Type 1 collagen, fibronectin 및 UNCL 발현에 미치는 H₂O₂와 chlorhexidine의 효과

Effects of H₂O₂ and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts

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Purpose: To evaulate the effects of chlorhexidine and H₂O₂ on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). Materials and Methods: 1.2×10-¹%, 1.2×10-²% and 1.2×10-³% CHX and 3x10-³%, 3×10-⁴% and 3x10-5% H₂O₂ and mixture of CHX and H₂O₂ were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2×10-³% CHX increased mRNA expressions of hPDLF as application time increased. 3. H₂O₂ lower than 3x10-³% increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2×10-¹% CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2×10-²% CHX (with or without H2O2) for 30 minutes resulted in no gene expression. Conclusion: Because low concentration of CHX and H₂O₂ increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect. (J Korean Acad Periodontol 2008;38:645-656)

Abstract

Purpose: To evaulate the effects of chlorhexidine and H₂O₂ on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). Materials and Methods: 1.2×10-¹%, 1.2×10-²% and 1.2×10-³% CHX and 3x10-³%, 3×10-⁴% and 3x10-5% H₂O₂ and mixture of CHX and H₂O₂ were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2×10-³% CHX increased mRNA expressions of hPDLF as application time increased. 3. H₂O₂ lower than 3x10-³% increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2×10-¹% CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2×10-²% CHX (with or without H2O2) for 30 minutes resulted in no gene expression. Conclusion: Because low concentration of CHX and H₂O₂ increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect. (J Korean Acad Periodontol 2008;38:645-656)

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관련 논문AI추천

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