사람 치주인대섬유모세포에서 MMP-1, TIMP-1,2, Type 1 collagen, fibronectin 및 UNCL 발현에 미치는 H₂O₂와 chlorhexidine의 효과
Effects of H₂O₂ and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Purpose: To evaulate the effects of chlorhexidine and H₂O₂ on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). Materials and Methods: 1.2×10-¹%, 1.2×10-²% and 1.2×10-³% CHX and 3x10-³%, 3×10-⁴% and 3x10-5% H₂O₂ and mixture of CHX and H₂O₂ were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2×10-³% CHX increased mRNA expressions of hPDLF as application time increased. 3. H₂O₂ lower than 3x10-³% increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2×10-¹% CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2×10-²% CHX (with or without H2O2) for 30 minutes resulted in no gene expression. Conclusion: Because low concentration of CHX and H₂O₂ increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect. (J Korean Acad Periodontol 2008;38:645-656)
Abstract
Purpose: To evaulate the effects of chlorhexidine and H₂O₂ on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). Materials and Methods: 1.2×10-¹%, 1.2×10-²% and 1.2×10-³% CHX and 3x10-³%, 3×10-⁴% and 3x10-5% H₂O₂ and mixture of CHX and H₂O₂ were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2×10-³% CHX increased mRNA expressions of hPDLF as application time increased. 3. H₂O₂ lower than 3x10-³% increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2×10-¹% CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2×10-²% CHX (with or without H2O2) for 30 minutes resulted in no gene expression. Conclusion: Because low concentration of CHX and H₂O₂ increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect. (J Korean Acad Periodontol 2008;38:645-656)
참고문헌 (39)
- Page RC, "The pathogenesis of human periodontitis: an introduction", Periodontol, 12, pp.9~11 (2000)
- Williams. RC, "Periodontal disease", New England J of Med, 322, pp.373~382 (1990)
- Battino M, "Oxidative injury and inflammatory periodontal diseases: the challenge of anti-oxidants to free radicals and reactive oxygen species", Crit Rev in Oral Biol Med, 10, pp.458~476 (1999)
- Chapple IL, "Reactive oxygen species and antioxidants in inflammatory diseases", J Clin Periodontol, 24, pp.287~296 (1997)
- Bonnefoy M, "Antioxidants to slow aging, facts and perspectives", Presse Med, 31, pp.1174~1184 (2002)
- Brecx M, "Effficacy of Listerine, Meridol and Chlorhexidine mouthrinses on plaque, gingivitis and plaque bacteria vitality", J Clin Periodontol, 17, pp.292~297 (1990)
- Walsh TF, "Clinical effects of pulsed oral irrigation with 0.2% chlorhexidine digluconate in patients with adult periodontitis", J Clin Periodontol, 19, pp.245~248 (1992)
- Giannelli M, "Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation", Toxicol In Vitro, 22, pp.308~317 (2008)
- Goultschin J, "Inhibition of superoxide generation by human polymorphonuclear leukocytes with chlorhexidine. Its possible relation to periodontal disease", J Periodontol, 57, pp.422~424 (1986)
- Battino M, "In vitro antioxidant activities of mouthrinses and their components", J Clin Periodontol, 29, pp.462~467 (2002)
- Firatli E, "Antioxidative activities of some chemotherapeutics. A possible mechanism in reducing gingival inflammation", J Clin Periodontol, 21, pp.680~683 (1994)
- Beighton D, "Effects of chlorhexidine on proteolytic and glycosidic enzyme activites of dental plaque bacteria", J Clin Periodontol, 18, pp.85~89 (1991)
- Cronan CA, "Inhibition of Porphyromonas gingivalis proteinases by chlorhexidine.: synergistic effect of Zn(II)", Oral Microbiol Immunol, 21, pp.212~217 (2006)
- Gendron R, "Inhibition of the activities of matrix metalloproteinases 2, 8 and 9 by chlorhexidine", Clin Diagn Lab Immunol, 6, pp.437~439 (1999)
- Cadosch J, "Root surface debridement and endotoxin removal", J Periodontal Res, 38, pp.229~236 (2003)
- Cabral CT, "In vitro comparison of chlorhexidine and povidone-iodine on the long-term proliferation and functional activity of human alveolar bone cells", Clin Oral Investing, 11, pp.155~164 (2007)
- Cline NV, "The effects of chlorhexidine on the attachment and growth of cultured human periodontal cells", J Periodontol, 63, pp.598~602 (1992)
- Pucher JJ, "The effects of chlorhexidine digluconate on human fibroblasts in vitro", J Periodontol, 63, pp.526~532 (1992)
- Alleyn CD, "The effect of chlorhexidine treatment of root surfaces on the attachment of human gingival fibroblasts in vitro", J Periodontol, 62, pp.434~438
- Eren K, "Monitoring of buccal epithelial cells by alkaline comet assay (single cell gel electrophoresis technique) in cytogenetic evaluation on chlorhexidine", Clin Oral Investing, 6, pp.150~154 (2002)
- 강정구, "Chlorhexidine과 Listerine이 인체 치은 섬유모세포의 활성에 미치는 영향", 대한치주과학회지, 25, pp.1~13 (1995)
- Yeung SY, "Antioxidant and pro-oxidant properties of chlorhexidine and its interaction with calcium hydroxide solutions", Int Endod J, 40, pp.837~844 (2007)
- Meikle MC, "Advances in understanding cell interactions in tissue resorption. Relevance to the pathogenesis of periodontal disease and a new hypothesis", J Oral Pathol, 15, pp.239~250 (1986)
- Bartold PM, "The effect of oxygen- derived free radicals on gingival proteoglycans and hyaluronic acid", J Peirodontal Res, 19, pp.390~400 (1984)
- Beaman L, "The role of oxygen and its derivatives in microbial pathogenesis and host defense", Annu Rev Microbiaol, 38, pp.27~48 (1984)
- Waddington RJ, "Reactive oxygen species: a potential role in the pathogenesis of peridontal diseases", Oral Dis, 6, pp.138~151 (2000)
- Halliwell B, "Free radicals in biology and medicine" (1989), Clarendon Press
- Sen Gupta S, "Induction of repair functions by hydrogen peroxide in Chinese hamster cells", Int J Radiat Biol Relat Stud Phys Chem Med, 53, pp.935~942 (1988)
- Bose K, "Chronic low dose exposure to hydrogen peroxide changes sensitivity of V79 cells to different damaging agents", Indian J Exp Biol, 41, pp.832~836 (2003)
- 최용선, "H2O2와 ascorbic acid가 사람 치주인대섬유모세포의 TIMP-2, Type 1 collagen, PDLs22 발현에 끼치는 효과", Journal of Periodontal & Implant Science, 37(4), pp.655~669 (2007), 대한치주과학회
- Park JC, "Isolation and characterization of cultured human periodontal ligament fibroblast-specific cDNAs", Biochem Biophys Res Commun, 282, pp.1145~1153 (2001)
- 김병옥, "분자생물학을 이용하여 복제노화된 사람치주인대섬유모세포의 세포학적 연구", Journal of Periodontal & Implant Science, 35(3), pp.623~634 (2005), 대한치주과학회
- Kubota T, "Altered gene expression levels of matrix metalloproteinases and their inhibitors in periodontitis- affected gingival tissue", J Periodontol, 79, pp.166~173 (2008)
- Dahan M, "Bolcato- Bellemin AL, Birembaut P. et. al. Expression of matrix metalloproteinases in healthy and diseased human gingiva", J Clin Periodontol, 28, pp.128~136 (2001)
- Hay ED, "Cell Biology of extracellular matrix", pp.21~ (1991), Plenum Press
- Khorramizadeh MR, "Aging differentially modulates the expresion of collagen and collagenase in dermal fibroblasts", Mol Cell Biochem, 194, pp.99~108 (1999)
- Kapila YL, "The response of periodontal ligament cells to fibronectin", J Peiodontol, 69(9), pp.1008~1019 (1998)
- Abiko Y, "Effect of aging on functional changes of periodontal tissue cells", Ann Peirodontol, 3, pp.350~369 (1998)
- Pitts G, "The in vivo effects of an antiseptic mouthwash on odor-producing microorganism", J Dent Res, 60, pp.1891~1896 (1981)
