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Prevotella intermedia의 세균내독소가 치은섬유아세포와 치주인대세포에서의 matrix metalloproteinase 및 tissue inhibitor of metalloproteinase의 발현에 미치는 영향

Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival and periodontal ligament fibroblasts treated with lipopolysaccharide from Prevotella ...

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참고문헌

주제분류치의학

초록

Abstract

Matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes and implicated in the remodeling and degradation of extracellular matrix under both physiological and pathological conditions. Connective tissue degradation in periodontal diseases is thought to be due to excessive MMP activities over their specific inhibitors. The effects of lipopolysaccharide (LPS) from Prevotella intermedia, one of the major putative pathogens of periodontitis, on the expression of mRNA for MMPs and tissue inhibitors of metalloproteinases (TIMPs) in human gingival and periodontal ligament fibroblasts were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNAs encoding MMP-1, -2, -3, -10, and -14 was increased in human gingival fibroblasts treated with P. intermedia LPS, whereas MMP-11 and TIMP-2 mRNA expression was decreased in these cells stimulated with LPS. P. intermedia LPS increased the MMP-1, -2, -10, -11, and -14 mRNA expression and decreased TIMP-1 and -2 mRNA expression in human periodontal ligament fibroblasts. These findings imply that P. intermedia LPS may play an important role in the connective tissue degradation in periodontitis.

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관련 논문AI추천

MMP and TIMP production in periodontal ligament fibroblasts stimulated by Prevotella nigrescens lipopolysaccharide김미리, 손호현, 양원경, 이우철대한치과보존학회 · Restorative Dentistry and Endodontics2005 · 제30권 제5호Prevotella nigrescens lipopolysaccharides로 자극된 MG63 세포에서 분비되는 기질금속단백질 MMP-1과 TIMP-1의 수준에 관한 연구김미리, 손원준, 손호현, 양원경, 엄정문, 이인복, 조병훈대한치과보존학회 · Restorative Dentistry and Endodontics2004 · 제29권 제5호치은섬유아세포의 MMP 발현에 대한 Nitric Oxide의 영향고정태, 신인식, 윤상오, 정현주대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호Treponema lecithinolyticum lipopolysaccharide에 의한 matrix metalloproteinase-9의 발현남정아, 문선영, 유윤정, 이진욱, 차정헌, 최봉규대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제3호사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현구영, 구기태, 김정은, 김태일, 류인철, 이성훈, 이용무, 정종평, 최봉규대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제3호Actinobacillus actinomycetemcomitans 내독소가?백서 치주조직에 미치는 영향김영준, 김종철, 최득철대한치주과학회 · Journal of Periodontal & Implant Science2007 · 제37권 제5호

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