영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화
Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
Abstract
Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
참고문헌 (28)
- Okeson JP, "Management of temporomandibular disorder5th edition", pp.15~22 (2003), Elsevier
- Arnett GW, "Progressive mandibular retrusion-idiopathic condylar resorption Part II", Am J Orthod Dentofacial Orthop, 110, pp.117~127 (1996)
- Arnett GW, "Progressive mandibular retrusion-idiopathic condylar resorption Part I", Am J Orthod Dentofacial Orthop, 110, pp.8~15 (1996)
- Wolford LM, "Idiopathic condylar resorption: diagnosis, treatment protocol, and outcomes", Am J Orthod Dentofacial Orthop, 116, pp.667~677 (1999)
- Crawford JG, "Stability after reoperation for progressive condylar resorption after orthognathic surgery: report of seven cases", J Oral Maxillofac Surg, 52, pp.460~466 (1994)
- De Clercq CA, "Condylar resorption in orthognathic surgery: a retrospective study", Int J Adult Orthodon Orthognath Surg, 9, pp.233~240 (1994)
- Merkx MA, "Condylar resorption after orthognathic surgery. Evaluation of treatment in 8 patients", J Craniomaxillofac Surg, 22, pp.53~58 (1994)
- Mcllwraith CW, "Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects", Arthroscopy, 27, pp.1552~1561 (2011)
- Meyerrose T, "Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors", Adv Drug Deliv Rev, 62, pp.1167~1174 (2010)
- van Buul GM, "Clinically translatable cell tracking and quantification by MRI in cartilage repair using superparamagnetic iron oxides", PLoS One, 6, pp.17001~ (2011)
- Zhang J, "Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity", BMC Med, 9, pp.68~ (2011)
- Gronthos S, "Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo", Proc Natl Acad Sci U S A, 97, pp.13625~13630 (2000)
- Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
- Huang AH, "Isolation and characterization of human dental pulp stem", stromal cells from nonextracted crown-fractured teeth requiring root canal therapy, 2009, pp.35, 673~681
- Pierdomenico L, "Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp", Transplantation, 80, pp.836~842 (2005)
- Struys T, "Ultrastructural and immunocytochemical analysis of multilineage differentiated human dental pulp- and umbilical cord-derived mesenchymal stem cells", Cells Tissues Organs, 193, pp.366~378 (2011)
- Ichinose S, "Morphological differences during in vitro chondrogenesis of bone marrow-, synovium-MSCs, and chondrocytes", Lab Invest, 90, pp.210~221 (2010)
- Huang GT, "In vitro characterization of human dental pulp cells: various isolation methods and culturing environments", Cell Tissue Res, 324, pp.225~236 (2006)
- "Determination of adipose-derived stem cell application on photo-aged fibroblasts, based on paracrine function", CYTOTHERAPY, 13(3), pp.378~384 (2011), TAYLOR & FRANCIS AS
- "The differentiation-inducing effect of conditioned media obtained from dental pulp cells", ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 107, pp.E54~E59 (2009)
- Dominici M, "Minimal criteria for defining multipotent mesenchymal stromal cells,In The International Society for Cellular Therapy position statement", Cytotherapy, 8, pp.315~317 (2006)
- Ahrens PB, "Stage-related capacity for limb chondrogenesis in cell culture", Dev Biol, 60, pp.69~82 (1977)
- Miura M, "SHED: stem cells from human exfoliated deciduous teeth", Proc Natl Acad Sci U S A, 100, pp.5807~5812 (2003)
- "Identification of novel epithelial stem cell-like cells in human deciduous dental pulp", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 386, pp.135~139 (2009), New York, Academic Press.
- 박정극, "Effects of Mechanical Stimulation on the Proliferation of Bone Marrow-derived Human Mesenchymal Stem Cells", Biotechnology and Bioprocess Engineering, 12(6), pp.601~609 (2007), 한국생물공학회
- 한미정, "Effect of Mechanical Tension on the Human Dental Pulp Cells", Biotechnology and Bioprocess Engineering, 13(4), pp.410~417 (2008), 한국생물공학회
- Tanaka K, "Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzymedigested culture methods", Arch Oral Biol, 56, pp.380~388 (2011)
- Mobasheri A, "Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy", Histol Histopathol, 24, pp.347~336 (2009)