성견 치계줄기세포 및 골수줄기세포 특성에 관한 연구
Investigation of postnatal stem cells from canine dental tissue and bone marrow
2
조회
0
다운로드
2
인용
0
참고문헌
초록
Purpose: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow. Methods: Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation. Results: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /β- tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation. Conclusions: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration. (J Korean Acad Periodontol 2009;39:119-128)
Abstract
Purpose: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow. Methods: Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation. Results: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /β- tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation. Conclusions: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration. (J Korean Acad Periodontol 2009;39:119-128)
참고문헌 (22)
- Cai J, "In search of “stemness”", Exp Hematol, 32, pp.585~598 (2004)
- Wagers AJ, "Plasticity of adult stem cells", Cell, 116, pp.639~648 (2004)
- Bianco P, "Stem cells in tissue engineering", Nature, 414, pp.118~121 (2001)
- Fuchs E, "Socializing with the neighbors: stem cells and their niche", Cell, 116, pp.769~778 (2004)
- Yin T, "The stem cell niches in bone", J Clin Invest, 116, pp.1195~1201 (2006)
- Bruder SP, "The effect of implants loaded with autogenous mesenchymal stem cells on the healing of canine segmental bone defects", J Bone and Joint Surg, 80(A), pp.986~996 (1998)
- Caplan AI, "Mesenchymal stem cells: cell-based reconstructive therapy in orthopedics", Tissue Engineering, 11, pp.1198~1211 (2005)
- Kawaguchi H, "Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells", J Periodontol, 75, pp.1281~1287 (2004)
- Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
- Matsunara T, "Alveolar bone marrow as a cell source for regenerative medicine: differences between alveolar and Iliac bone marrow stromal cells", J Bone Miner Res, 20, pp.399~409 (2005)
- Liu Y, "Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine", Stem Cells, 26, pp.1065~1073 (2008)
- 김경화, "성견 치주인대세포의 줄기세포 특성 연구", The Journal of the Korean Academy of Periodontology (JPIS), 37(3), pp.479~488 (2007), 대한치주과학회
- Bruder SP, "Monoclonal antibodies reactive with human osteogenic cell surface antigens", Bone, 21, pp.225~235 (1997)
- Tsutsumi S, "Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF", Biochem Biophys Res Commun, 288, pp.413~419 (2001)
- Nagatomo K, "Stem cell properties of human periodontal ligament cells", J Periodontal Res, 41, pp.303~310 (2006)
- Inoue T, "Induction of chondrogenesis in muscle, skin, bone marrow, and periodontal ligament by demineralized dentin and bone matrix in vivo and in vitro", J Dent Res, 65, pp.12~22 (1986)
- Jo YY, "Isolation and characterization of postnatal stem cells from human dental tissues", Tissue Engineering, 13, pp.767~773 (2007)
- Singhatanadgit W, "Isolation and characterization of stem cell clones from adult human ligament", Tissue Engineering Part A (2009)
- Ivanovski S, "Stem cells in the periodontal ligament", Oral Diseases, 12, pp.358~363 (2006)
- Lee YM, "Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices", J Biomed Mater Res, 54, pp.216~223 (2001)
- Park DJ, "Injectable bone using chitosan-alginate gel", mesenchymal stem cells, Journal of Cranio-Maxillofacial Surgery, pp.2005, 33[DOI]
- Hasegawa N, "Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects", J Periodontol, 77, pp.1003~1007 (2006)