An Immune-compromised Method for Tooth Transplantation Using Adult Bone Marrow Stromal Cells and Embryonic Tooth Germ
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Tooth transplantation using autogenic adult teeth or embryonic tooth germs is the one of best treatments for replacement of missing teeth, but there are limitations in the accessibility. Isogenic or xenogenic tooth transplantation has been failed because of the immune rejection response occurring in the periodontal ligament of transplanted tooth. In this study, by utilizing the recombination between mouse embryonic tooth germ and mouse adult bone marrow stromal cells, we tried to replace the periodontal tissues such as periodontal ligament and alveolar bone with adult bone marrow stromal cells. At four weeks after the transplantation of the recombinant into a kidney,adult bone marrow-derived cells cells were observed in the periodontal ligament and alveolar bone. This result indicates that adult bone marrow stromal cells can participate in the formation of periodontal tissues. If these tooth and periodontal tissues are transplanted into host who donates adult bone marrow stromal cells, adult bone marrowderived cells will be regarded as host cells, and immune rejection response will not occur in these cells. Therefore,it is suggested that recombination between adult bone marrow stromal cells and embryonic tooth germ is a good candidate method using xenogenic tooth germ for replacement of missing teeth in human by replacing cells in periodontal tissues with human adult bone marrow stromal cells.
Abstract
Tooth transplantation using autogenic adult teeth or embryonic tooth germs is the one of best treatments for replacement of missing teeth, but there are limitations in the accessibility. Isogenic or xenogenic tooth transplantation has been failed because of the immune rejection response occurring in the periodontal ligament of transplanted tooth. In this study, by utilizing the recombination between mouse embryonic tooth germ and mouse adult bone marrow stromal cells, we tried to replace the periodontal tissues such as periodontal ligament and alveolar bone with adult bone marrow stromal cells. At four weeks after the transplantation of the recombinant into a kidney,adult bone marrow-derived cells cells were observed in the periodontal ligament and alveolar bone. This result indicates that adult bone marrow stromal cells can participate in the formation of periodontal tissues. If these tooth and periodontal tissues are transplanted into host who donates adult bone marrow stromal cells, adult bone marrowderived cells will be regarded as host cells, and immune rejection response will not occur in these cells. Therefore,it is suggested that recombination between adult bone marrow stromal cells and embryonic tooth germ is a good candidate method using xenogenic tooth germ for replacement of missing teeth in human by replacing cells in periodontal tissues with human adult bone marrow stromal cells.
참고문헌 (26)
- Akahane M, "Hydroxyapatite ceramics as a carrier of gene-transduced bone marrow cells", J Orthop Sci, 7, pp.677~682 (2002)
- Bell P, "An optimized protocol for detection of E. coli beta-galactosidase in lung tissue following gene transfer", Histochem Cell Biol, 124, pp.77~85 (2005)
- 김재영, "Lineage of non-cranial neural crest cell in the dental mesenchyme: using a lacZ reporter gene during early tooth development", JOURNAL OF ELECTRON MICROSCOPY, 52, pp.567~571 (2003), OXFORD UNIV PRESS
- Coates PJ, "Induction of endogenous beta-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice", Oncogene, 20, pp.7096~7097 (2001)
- Duailibi SE, "Bioengineered dental tissues grown in the rat jaw", J Dent Res, 87, pp.745~750 (2008)
- Gronthos S, "Boyde A, DenBesten P, Robey PG, Shi S: Stem cell properties of human dental pulp stem cells", J Dent Res, 81, pp.531~535 (2002)
- Hu B, "Tissue engineering of tooth crown, root, and periodontium", Tissue Eng, 12, pp.2069~2075 (2006)
- Ikeda E, "Fully functional bioengineered tooth replacement as an organ replacement therapy", Proc Natl Acad Sci USA, 106, pp.13475~13480 (2009)
- Kopp HG, "Beta-galactosidase staining on bone marrow. The osteoclast pitfall", Histol Histopathol, 22, pp.971~976 (2007)
- Huang AT, "Regeneration of dentin-pulp complex with cementum and periodontal ligament formation using dental bud cells in gelatinchondroitinhyaluronan tri-copolymer scaffold in swine", J Biomed Mater Res A, 86, pp.1062~1068 (2008)
- Mantesso A, "Dental stem cells for tooth regeneration and repair", Expert Opin Biol Ther, 9, pp.1143~1154 (2009)
- Nakao K, "The development of a bioengineered organ germ method", Nat Methods, 4, pp.227~230 (2007)
- Ohazama A, "Stem-cellbased tissue engineering of murine teeth", J Dent Res, 83, pp.518~522 (2004)
- Odgren PR, "False-positive beta-galactosidase staining in osteoclasts by endogenous enzyme: studies in neonatal and month-old wild-type mice", Connect Tissue Res, 47, pp.229~234 (2006)
- Owen M, "Marrow stromal cells", Journal of Cell Science, 10, pp.63~76 (1988)
- Robinson PJ, "Evidence of the alloimmunogenic potential of donor periodontal ligament", Am J Pathol, 75, pp.503~512 (1974)
- Rosenberg WS, "Authentic and artifactual detection of the E. coli lacZ gene product in the rat brain by histochemical methods", Res Mol Brain Res, 16, pp.311~315 (1992)
- Seo BM, "Recovery of stem cells from cryopreserved periodontal ligament", J Dent Res, 84, pp.907~912 (2005)
- Song AM, "A study of enamel matrix proteins on differentiation of porcine bone marrow stromal cells into cementoblasts", Cell Prolif, 40, pp.381~396 (2007)
- Sonoyama W, "Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study", J Endod, 34, pp.166~171 (2008)
- Teitelbaum SL, "Bone resorption by osteoblasts", Science, 289, pp.1504~1508 (2000)
- Ten Cate AR, "The development of the periodontium-a largely ectomesenchymally derived unit", Periodontol 2000, 13, pp.9~19 (1997)
- Van der Weijden F, "lveolar bone dimensional changes of post-extraction sockets in humans: a systematic review", J Clin Periodontol, 36, pp.1048~1058 (2009)
- Yang Y, "Periodontal regeneration using engineered bone marrow mesenchymal stromal cells", Biomaterials, 31, pp.8574~8582 (2010)
- Young CS, "Tissue engineering of complex tooth structures on biodegradable polymer scaffolds", J Dent Res, 81, pp.695~700 (2002)
- Yen AH, "Regeneration of teeth using stem cell-based tissue engineering", Expert Opin Biol Ther, 6, pp.9~16 (2006)