Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Purpose: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. Methods: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells)and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method. Results: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups. Conclusions: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.
Abstract
Purpose: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. Methods: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells)and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method. Results: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups. Conclusions: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.
참고문헌 (28)
- Di Nicola M, "Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli", Blood, 99, pp.3838~3843 (2002)
- Liu J, "Suppression of human peripheral blood lymphocyte proliferation by immortalized mesenchymal stem cells derived from bone marrow of Banna Minipig inbred-line", Transplant Proc, 36, pp.3272~3275 (2004)
- Bernardo ME, "Mesenchymal stromal cells", Ann N Y Acad Sci, 1176, pp.101~117 (2009)
- Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
- Sonoyama W, "Mesenchymal stem cell-mediated functional tooth regeneration in swine", PLoS One, 1, pp.e79~ (2006)
- Sonoyama W, "Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study", J Endod, 34, pp.166~171 (2008)
- Tsutsumi S, "Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF", Biochem Biophys Res Commun, 288, pp.413~419 (2001)
- Liu D, "Statistical determination of threshold for cellular division in the CFSE-labeling assay", J Immunol Methods, 312, pp.126~136 (2006)
- Lin NH, "Stem cells and future periodontal regeneration", Periodontol 2000, 51, pp.239~251 (2009)
- 진민주, "성견 치계줄기세포 및 골수줄기세포 특성에 관한 연구", Journal of Periodontal & Implant Science, 39(2), pp.119~128 (2009), 대한치주과학회
- Kim SH, "Alveolar bone regeneration by transplantation of periodontal ligament stem cells and bone marrow stem cells in a canine peri-implant defect model: A pilot study", Journal of Periodontology, 80(11), pp.1815~1823 (2009), American Academy of Periodontology
- Choumerianou DM, "Stem cells: promises versus limitations", Tissue Eng Part B Rev, 14, pp.53~60 (2008)
- Wada N, "Immunomodulatory properties of human periodontal ligament stem cells", J Cell Physiol, 219, pp.667~676 (2009)
- Nauta AJ, "Immunomodulatory properties of mesenchymal stromal cells", Blood, 110, pp.3499~3506 (2007)
- Le Blanc K, "HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells", Exp Hematol, 31, pp.890~896 (2003)
- "Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals", Blood, 102, pp.3837~3844 (2003)
- Puissant N, "Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells", Brit J Haematol, 129, pp.118~129 (2005)
- Bruder SP, "Monoclonal antibodies reactive with human osteogenic cell surface antigens", Bone, 21, pp.225~235 (1997)
- Nagatomo K, "Stem cell properties of human periodontal ligament cells", J Periodontal Res, 41, pp.303~310 (2006)
- Rasmusson I, "Mesenchymal stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T lymphocytes or natural killer cells", Transplantation, 76, pp.1208~1213 (2003)
- Tse WT, "Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation", Transplantation, 75, pp.389~397 (2003)
- Sotiropoulou PA, "Papamichail M. Interactions between human mesenchymal stem cells and natural killer cells", Stem Cells, 24, pp.74~85 (2006)
- Beyth S, "Human mesenchymal stem cells alter antigen- presenting cell maturation and induce T-cell unresponsiveness", Blood, 105, pp.2214~2219 (2005)
- Poznansky MC, "Active movement of T cells away from a chemokine", Nat Med, 6, pp.543~548 (2000)
- Theill LE, "RANK-L and RANK: T cells, bone loss, and mammalian evolution", Annu Rev Immunol, 20, pp.795~823 (2002)
- Roper RL, "Prostaglandin E2 inhibits B lymphocyte activation by a cAMP-dependent mechanism: PGE-inducible regulatory proteins", Cell Immunol, 154, pp.296~308 (1994)
- Frumento G, "Tryptophan-derived catabolites are responsible for inhibition of T and natural killer cell proliferation induced by indoleamine 2,3-dioxygenase", J Exp Med, 196, pp.459~468 (2002)
- Rasmusson I, "Immune modulation by mesenchymal stem cells", Experimental Cell Research, 312, pp.2169~2179 (2006)