Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
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Objectives: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. Materials and Methods: Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd). Results: Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae. Conclusions: Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules.
Abstract
Objectives: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. Materials and Methods: Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd). Results: Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae. Conclusions: Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules.
참고문헌 (34)
- Kakehashi S, "The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats", Oral Surg Oral Med Oral Pathol, 20, pp.340~349 (1965)
- Akpata ES, "Blechman H. Bacterial invasion of pulpal dentin wall in vitro", J Dent Res, 61, pp.435~438 (1982)
- Haapasalo M, "In vitro infection and disinfection of dentinal tubules", J Dent Res, 66, pp.1375~1379 (1987)
- Ricucci D, "Fate of the tissue in lateral canals and apical ramifications in response to pathologic conditions and treatment procedures", J Endod, 36, pp.1~15 (2010)
- Engstrom B, "The correlation between positive culture and the prognosis of root canal therapy after pulpectomy", Odontol Revy, 16, pp.193~203 (1965)
- Nair PN, "Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscope follow-up study", J Endod, 16, pp.580~588 (1990)
- Sen BH, "Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM", Endod Dent Traumatol, 11, pp.6~9 (1995)
- Armitage GC, "Cemental changes in teeth with heavily infected root canals", J Endod, 9, pp.127~130 (1983)
- Orstavik D, "Disinfection by endodontic irrigants and dressing of experimentally infected dentinal tubules", Endod Dent Traumatol, 6, pp.142~149 (1990)
- Valera MC, "Effect of sodium hypochlorite and five intracanal medications on Candida albicans in root canals", J Endod, 27, pp.401~403 (2001)
- Saleh IM, "Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers in vitro", Int Endod J, 37, pp.193~198 (2004)
- Gurgel-Filho ED, "In vitro evaluation of the effectiveness of the chemomechanical preparation against Enterococcus faecalis after single- or multiple-visit root canal treatment", Braz Oral Res, 21, pp.308~313 (2007)
- Michelich VJ, "Pashley DH. Bacterial Penetration of human dentin in vitro", J Dent Res, 59, pp.1398~1403 (1980)
- Siqueira JF Jr., "Aetiology of root canal treatment failure: why well-treated teeth can fail", Int Endod J, 34, pp.1~10 (2001)
- 이연재, "급성 치수염 및 급성 치근단 농양의 치근관으로부터의 세균 분리 및 동정", Restorative Dentistry and Endodontics, 30(5), pp.409~422 (2005), 대한치과보존학회
- Siqueira JF Jr, "Clinical implications and microbiology of bacterial persistence after treatment procedures", J Endod, 34 (2008)
- Poptani B, "Detection of Enterococcus faecalis and Candida albicans in previously root‑filled teeth in a population of Gujarat with polymerase chain reaction", Contemp Clin Dent, 4, pp.62~66 (2013)
- 김현식, "Effects of Enterococcus faecalis sonicated extracts on IL-2, IL-4 and TGF-β1 production from human lymphocytes", Restorative Dentistry and Endodontics, 30(1), pp.1~6 (2005), 대한치과보존학회
- Peciuliene V, "Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis", Int Endod J, 34, pp.429~434 (2001)
- Molander A, "Microbiological status of root-filled teeth with apical periodontitis", Int Endod J, 31, pp.1~7 (1998)
- 김혜정, "이염화이소시아뉼산나트륨 제재의 근관 내 사용 시 시간에 따른 E. faecalis에 대한 항균성 평가", Restorative Dentistry and Endodontics, 32(2), pp.121~129 (2007), 대한치과보존학회
- Love RM., "Enterococcus faecalis-a mechanism for its role in endodontic failure", Int Endod J, 34, pp.399~405 (2001)
- Zapata RO, "Confocal laser scanning microscopy is appropriate to detect viability of Enterococcus faecalis in infected dentin", J Endod, 34, pp.1198~1201 (2008)
- Sen BH, "Growth patterns of Candida albicans in relation to radicular dentin", Oral Surg Oral Med Oral Path Oral Radiol Endod, 84, pp.68~73 (1997)
- Hagihara Y, "Degradation of human dentine collagen by an enzyme produced by the yeast Candida albicans", Arch Oral Biol, 33, pp.617~619 (1988)
- Sen BH, "Colonisation of Candida albicans on cleaned human dental hard tissues", Arch Oral Biol, 42, pp.513~520 (1997)
- Waltimo TM, "Haapasalo MP", J Endod, 26, pp.207~209 (2000)
- Siqueira JF Jr, "Fungal infection of the radicular dentin", J Endod, 28, pp.770~773 (2002)
- 전인수, "수종의 엔진 구동형 니켈-타이타늄 합금파일의 절삭각에 따른 근관성형 효과에 관한 전자현미경적 연구", Restorative Dentistry and Endodontics, 27(6), pp.577~586 (2002), 대한치과보존학회
- Hulsmann M, "A comparative study of root canal preparation with HERO 642 and Quantec SC rotary Ni-Ti instruments", Int Endod J, 34, pp.538~546 (2001)
- Biesterfeld RC, "A comparison of periapical seals of root canals with RC-Prep or Salvizol", Oral Surg Oral Med Oral Path, 49, pp.532~537 (1980)
- Meryon SD, "Penetration of dentine by three oral bacteria in vitro and their associated cytotoxicity", Int Endod J, pp.196~202 (1990)
- Ando N, "Predominant obligate anaerobes invading the deep layers of root canal dentine", Int Endod J, 23, pp.20~27 (1990)
- Peters LB, "Viable bacteria in root dentinal tubules of teeth with apical periodontitis", J Endod, 27, pp.76~81 (2001)