Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: This study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols. Materials and Methods: Sixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3).Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student’s t-test were used for data analysis, with a significance level of 5%. Results: The NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019). Conclusions: The NaOCl penetration depth was similar in groups with root canal preparation.Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.
Abstract
Objectives: This study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols. Materials and Methods: Sixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3).Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student’s t-test were used for data analysis, with a significance level of 5%. Results: The NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019). Conclusions: The NaOCl penetration depth was similar in groups with root canal preparation.Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.
참고문헌 (38)
- Murray PE, "Regenerative endodontics : a review of current status and a call for action", J Endod, 33, pp.377~390 (2007)
- Banchs F, "Revascularization of immature permanent teeth with apical periodontitis : new treatment protocol?", J Endod, 30, pp.196~200 (2004)
- Kim SG, "Regenerative endodontics : a comprehensive review", Int Endod J, 51, pp.1367~1388 (2018)
- Iwaya SI, "Revascularization of an immature permanent tooth with apical periodontitis and sinus tract", Dent Traumatol, 17, pp.185~187 (2001)
- Jeeruphan T, "Mahidol study 1 : comparison of radiographic and survival outcomes of immature teeth treated with either regenerative endodontic or apexification methods : a retrospective study", J Endod, 38, pp.1330~1336 (2012)
- "AAE clinical considerations for a regenerative procedure"[URL]
- Galler KM, "European Society of Endodontology position statement : revitalization procedures", Int Endod J, 49, pp.717~723 (2016)
- Verma P, "Effect of residual bacteria on the outcome of pulp regeneration in vivo", J Dent Res, 96, pp.100~106 (2017)
- Vishwanat L, "Effect of bacterial biofilm on the osteogenic differentiation of stem cells of apical papilla", J Endod, 43, pp.916~922 (2017)
- Lee C, "Failure of regenerative endodontic procedures : case analysis and subsequent treatment options", J Endod, 48, pp.1137~1145 (2022)
- Martin DE, "Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation", J Endod, 40, pp.51~55 (2014)
- Elnaggar SE, "Effect of different irrigation protocols for applications in regenerative endodontics on mechanical properties of root dentin", Aust Endod J, 47, pp.228~235 (2021)
- Zeng Q, "Release of growth factors into root canal by irrigations in regenerative endodontics", J Endod, 42, pp.1760~1766 (2016)
- Galler KM, "Penetration depth of irrigants into root dentine after sonic, ultrasonic and photoacoustic activation", Int Endod J, 52, pp.1210~1217 (2019)
- Virdee SS, "The influence of irrigant activation, concentration and contact time on sodium hypochlorite penetration into root dentine: an ex vivo experiment", Int Endod J, 53, pp.986~997 (2020)
- Tungsawat P, "Comparison of the effect of three irrigation techniques and root canal preparation size on sodium hypochlorite penetration into root canal dentinal tubules", Int J Dent, 2021, pp.6612588~ (2021)
- Sasanakul P, "Influence of adjuncts to irrigation in the disinfection of large root canals", J Endod, 45, pp.332~337 (2019)
- Dos Reis S, "Volumetric analysis of irrigant extrusion in immature teeth after different final agitation techniques", J Endod, 46, pp.682~687 (2020)
- Faria G, "Penetration of sodium hypochlorite into root canal dentine: effect of surfactants, gel form and passive ultrasonic irrigation", Int Endod J, 52, pp.385~392 (2019)
- van der Sluis LW, "Study on the influence of refreshment", activation cycles and irrigants on mechanical cleaning efficiency during ultrasonic activation of the irrigant, 2010, pp.36, 737~740
- Haapasalo M, "In vitro infection and disinfection of dentinal tubules", J Dent Res, 66, pp.1375~1379 (1987)
- Brittan JL, "In vivo model for microbial invasion of tooth root dentinal tubules", J Appl Oral Sci, 24, pp.126~135 (2016)
- Kakoli P, "The effect of age on bacterial penetration of radicular dentin", J Endod, 35, pp.78~81 (2009)
- Kontakiotis EG, "A quantitative and diametral analysis of human dentinal tubules at pulp chamber ceiling and floor under scanning electron microscopy", Aust Endod J, 41, pp.29~34 (2015)
- Gründling GL, "Effect of ultrasonics on Enterococcus faecalis biofilm in a bovine tooth model", J Endod, 37, pp.1128~1133 (2011)
- Schilke R, "Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation", Arch Oral Biol, 45, pp.355~361 (2000)
- Camargo CH, "Topographical, diametral, and quantitative analysis of dentin tubules in the root canals of human and bovine teeth", J Endod, 33, pp.422~426 (2007)
- Schmalz G, "Permeability characteristics of bovine and human dentin under different pretreatment conditions", J Endod, 27, pp.23~30 (2001)
- Gu Y, "Effect of different agitation techniques on the penetration of irrigant and sealer into dentinal tubules", Photomed Laser Surg, 35, pp.71~77 (2017)
- Sekar Vadhana, "Evaluation of penetration depth of 2% chlorhexidine digluconate into root dentinal tubules using confocal laser scanning microscope", Restorative Dentistry and Endodontics, 40(2), pp.149~154 (2015), 대한치과보존학회
- Zou L, "Penetration of sodium hypochlorite into dentin", J Endod, 36, pp.793~796 (2010)
- Violich DR, "The smear layer in endodontics-a review", Int Endod J, 43, pp.2~15 (2010)
- Lui JN, "Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer", J Endod, 33, pp.472~475 (2007)
- Kato AS, "Investigation of the efficacy of passive ultrasonic irrigation versus irrigation with reciprocating activation : an environmental scanning electron microscopic study", J Endod, 42, pp.659~663 (2016)
- Tay FR, "Effect of vapor lock on root canal debridement by using a side-vented needle for positive-pressure irrigant delivery", J Endod, 36, pp.745~750 (2010)
- Castelo-Baz P, "In vitro comparison of passive and continuous ultrasonic irrigation in simulated lateral canals of extracted teeth", J Endod, 38, pp.688~691 (2012)
- Boutsioukis C, "Present status and future directions-irrigants and irrigation methods", Int Endod J, 55(Supplement 3), pp.588~612 (2022)
- Wagner MH, "Final irrigation protocols may affect intraradicular dentin ultrastructure", Clin Oral Investig, 21, pp.2173~2182 (2017)
