Development of a mouse model for pulp-dentin complex regeneration research: a preliminary study
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Objectives: To achieve pulp-dentin complex regeneration with tissue engineering, treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals. Mice have been a species of choice in which to study stem cell biology in mammals. However, most pulp-dentin complex regeneration studies have used large animals because the mouse tooth is too small. The purpose of this study was to demonstrate the utility of the mouse tooth as a transplantation model for pulp-dentin complex regeneration research. Materials and Methods: Experiments were performed using 7-week-old male Institute of Cancer Research (ICR) mice; a total of 35 mice had their pulp exposed, and 5 mice each were sacrificed at 1, 2, 4, 7, 9, 12 and 14 days after pulp exposure. After decalcification in 5% ethylenediaminetetraacetic acid, the samples were embedded and cut with a microtome and then stained with hematoxylin and eosin. Slides were observed under a high-magnification light microscope. Results: Until 1 week postoperatively, the tissue below the pulp chamber orifice appeared normal. The remaining coronal portion of the pulp tissue was inflammatory and necrotic. After 1 week postoperatively, inflammation and necrosis were apparent in the root canals inferior to the orifices. The specimens obtained after experimental day 14 showed necrosis of all tissue in the root canals. Conclusions: This study could provide opportunities for researchers performing in vivo orthotopic transplantation experiments with mice.
Abstract
Objectives: To achieve pulp-dentin complex regeneration with tissue engineering, treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals. Mice have been a species of choice in which to study stem cell biology in mammals. However, most pulp-dentin complex regeneration studies have used large animals because the mouse tooth is too small. The purpose of this study was to demonstrate the utility of the mouse tooth as a transplantation model for pulp-dentin complex regeneration research. Materials and Methods: Experiments were performed using 7-week-old male Institute of Cancer Research (ICR) mice; a total of 35 mice had their pulp exposed, and 5 mice each were sacrificed at 1, 2, 4, 7, 9, 12 and 14 days after pulp exposure. After decalcification in 5% ethylenediaminetetraacetic acid, the samples were embedded and cut with a microtome and then stained with hematoxylin and eosin. Slides were observed under a high-magnification light microscope. Results: Until 1 week postoperatively, the tissue below the pulp chamber orifice appeared normal. The remaining coronal portion of the pulp tissue was inflammatory and necrotic. After 1 week postoperatively, inflammation and necrosis were apparent in the root canals inferior to the orifices. The specimens obtained after experimental day 14 showed necrosis of all tissue in the root canals. Conclusions: This study could provide opportunities for researchers performing in vivo orthotopic transplantation experiments with mice.
참고문헌 (16)
- Schilder H, "Cleaning and shaping the root canal", Dent Clin North Am, 18, pp.269~296 (1974)
- Shi S, "The efficacy of mesenchymal stem cells to regenerate and repair dental structures", Orthod Craniofac Res, 8, pp.191~199 (2005)
- Huang GT, "Stem", progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model, 2010, pp.16, 605~615
- Steindorff MM, "Innovative approaches to regenerate teeth by tissue engineering", Arch Oral Biol, 59, pp.158~166 (2014)
- Asutay F, "The effects of dental pulp stem cells on bone regeneration in rat calvarial defect model : micro-computed tomography and histomorphometric analysis", Arch Oral Biol, 60, pp.1729~1735 (2015)
- Kim S, "In vivo experiments with dental pulp stem cells for pulp-dentin complex regeneration", Mediators Inflamm, 2015, pp.409347~ (2015)
- Altaii M, "Standardisation of sheep model for endodontic regeneration", revitalisation research, 2016, pp.65, 87~94
- Tziafas D, "Dentine-pulp tissue engineering in miniature swine teeth by set calcium silicate containing bioactive molecules", Arch Oral Biol, 73, pp.230~236 (2017)
- Yu C, "An overview of the dental pulp : its functions and responses to injury", Aust Dent J, 52, pp.S4~S16 (2007)
- Abbott PV, "A clinical classification of the status of the pulp and the root canal system", Aust Dent J, 52, pp.S17~S31 (2007)
- Bergenholtz G, "Effect of bacterial products on inflammatory reactions in the dental pulp", Scand J Dent Res, 85, pp.122~129 (1977)
- Ricucci D, "Endodontology: an integrated biological and clinical view" (2013), Quintessence Publishing Co. Ltd
- Heyeraas KJ, "Interstitial fluid pressure in normal and inflamed pulp", Crit Rev Oral Biol Med, 10, pp.328~336 (1999)
- Goldberg M, "The dental pulp: biology, pathology, and regenerative therapies" (2013), Springer
- Hahn CL, "Update on the adaptive immune responses of the dental pulp", J Endod, 33, pp.773~781 (2007)
- Renard E, "Immune cells and molecular networks in experimentally induced pulpitis", J Dent Res, 95, pp.196~205 (2016)