Bacterial cellulose matrix and acellular dermal matrix seeded with fibroblasts grown in platelet-rich plasma supplemented medium, compared to free gingival grafts: a randomized animal study
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Mucogingival defects (MGDs), such as dental root recessions, decreased vestibular depth, and absence of keratinized tissues, are commonly seen in dental clinics. MGDs may result in functional, aesthetic, and hygienic concerns. In these situations, autogenous soft tissue grafts are considered the gold-standard treatment. This study compares the healing process of free gingival grafts (FGGs) to bacterial cellulose matrix (BCM) and human acellular dermal matrix (ADM) seeded with fibroblasts from culture supplemented with platelet-rich plasma in a rat model. Methods: Surgical defects were made in rats, which received the following treatments in a randomized manner: group I, negative control (defect creation only); group II, positive control (FGG); group III, BCM; group IV, BCM + fibroblasts; group V, ADM; and group VI, ADM + fibroblasts. Clinical, histological, and immunological analyses were performed 15 days after grafting. Clinical examinations recorded epithelium regularity and the presence of ulcers, erythema, and/or edema. Results: The histological analysis revealed the degree of reepithelization, width, regularity, and presence of keratin. The Fisher exact statistical test was applied to the results (P<0.05). No groups showed ulcers except for group I. All groups had regular epithelium without erythema and without edema. Histologically, all groups exhibited regular epithelium with keratinization, and myofibroblasts were present in the connective tissue. The groups that received engineered grafts showed similar clinical and histological results to the FGG group. Conclusions: Within the limitations of this study, it was concluded that BCM and ADM can be used as cell scaffolds, with ADM yielding the best results. This study supports the use of this technical protocol in humans.
Abstract
Purpose: Mucogingival defects (MGDs), such as dental root recessions, decreased vestibular depth, and absence of keratinized tissues, are commonly seen in dental clinics. MGDs may result in functional, aesthetic, and hygienic concerns. In these situations, autogenous soft tissue grafts are considered the gold-standard treatment. This study compares the healing process of free gingival grafts (FGGs) to bacterial cellulose matrix (BCM) and human acellular dermal matrix (ADM) seeded with fibroblasts from culture supplemented with platelet-rich plasma in a rat model. Methods: Surgical defects were made in rats, which received the following treatments in a randomized manner: group I, negative control (defect creation only); group II, positive control (FGG); group III, BCM; group IV, BCM + fibroblasts; group V, ADM; and group VI, ADM + fibroblasts. Clinical, histological, and immunological analyses were performed 15 days after grafting. Clinical examinations recorded epithelium regularity and the presence of ulcers, erythema, and/or edema. Results: The histological analysis revealed the degree of reepithelization, width, regularity, and presence of keratin. The Fisher exact statistical test was applied to the results (P<0.05). No groups showed ulcers except for group I. All groups had regular epithelium without erythema and without edema. Histologically, all groups exhibited regular epithelium with keratinization, and myofibroblasts were present in the connective tissue. The groups that received engineered grafts showed similar clinical and histological results to the FGG group. Conclusions: Within the limitations of this study, it was concluded that BCM and ADM can be used as cell scaffolds, with ADM yielding the best results. This study supports the use of this technical protocol in humans.
참고문헌 (30)
- Clavero J, "Ramus or chin grafts for maxillary sinus inlay and local onlay augmentation : comparison of donor site morbidity and complications", Clin Implant Dent Relat Res, 5, pp.154~160 (2003)
- Broome WC, "Free autogenous connective tissue grafting : report of two cases", J Periodontol, 47, pp.580~585 (1976)
- Caffesse RG, "Treatment of localized gingival recessions. Part II. Coronally repositioned flap with a free gingival graft", J Periodontol, 49, pp.357~361 (1978)
- Callan DP, "Use of acellular dermal matrix for increasing keratinized tissue around teeth and implants", Pract Periodontics Aesthet Dent, 10, pp.731~734 (1998)
- Dorfman HS, "Longitudinal evaluation of free autogenous gingival grafts", J Clin Periodontol, 7, pp.316~324 (1980)
- Edel A, "The use of a free connective tissue graft to increase the width of attached gingiva", Oral Surg Oral Med Oral Pathol, 39, pp.341~346 (1975)
- Haeri A, "Mucogingival surgical procedures : a review of the literature", Quintessence Int, 30, pp.475~483 (1999)
- Marx RE, "Bone structure, metabolism, and physiology : its impact on dental implantology", Implant Dent, 7, pp.267~276 (1998)
- Ueda M, "Current status of tissue engineering industry in Japan and U. S", Tanpakushitsu Kakusan Koso, 45, pp.2342~2347 (2000)
- Breault LG, "Retained free gingival graft rugae : a 9-year case report", J Periodontol, 70, pp.438~440 (1999)
- Hall WB, "Present status of soft tissue grafting", J Periodontol, 48, pp.587~597 (1977)
- Silverstein LH, "Fundamentally changing soft tissue grafting", Dent Today, 16, pp.56~59 (1997)
- Sullivan HC, "Free autogenous gingival grafts. I. Principles of successful grafting", Periodontics, 6, pp.121~129 (1968)
- Kriegebaum U, "Tissue engineering of human oral mucosa on different scaffolds : in vitro experiments as a basis for clinical applications", Oral Surg Oral Med Oral Pathol Oral Radiol, 114, pp.S190~S198 (2012)
- Ophof R, "Implantation of tissue-engineered mucosal substitutes in the dog palate", Eur J Orthod, 30, pp.1~9 (2008)
- Ten Cate AR, "Oral histology: development, structure and function", pp.90~105 (1989), C.V. Mosby
- Prato GP, "An autologous cell hyaluronic acid graft technique for gingival augmentation : a case series", J Periodontol, 74, pp.262~267 (2003)
- Ferreira CF, "Platelet-rich plasma influence on human osteoblasts growth", Clin Oral Implants Res, 16, pp.456~460 (2005)
- Fontana JD, "Acetobacter cellulose pellicle as a temporary skin substitute", Appl Biochem Biotechnol, 24-25, pp.253~264 (1990)
- Sanchavanakit N, "Growth of human keratinocytes and fibroblasts on bacterial cellulose film", Biotechnol Prog, 22, pp.1194~1199 (2006)
- Zhang X, "Expansion and delivery of human fibroblasts on micronized acellular dermal matrix for skin regeneration", Biomaterials, 30, pp.2666~2674 (2009)
- Feng Y, "Differentiation of mesenchymal stem cells into neuronal cells on fetal bovine acellular dermal matrix as a tissue engineered nerve scaffold", Neural Regen Res, 9, pp.1968~1978 (2014)
- Rennert RC, "Cellular response to a novel fetal acellular collagen matrix : implications for tissue regeneration", Int J Biomater, 2013, pp.527957~ (2013)
- Wynn TA, "Macrophages in tissue repair, regeneration, and fibrosis", Immunity, 44, pp.450~462 (2016)
- Devereaux J, "Effects of platelet-rich plasma and platelet-poor plasma on human dermal fibroblasts", Maturitas, 117, pp.34~44 (2018)
- Caffesse RG, "Healing of free gingival grafts with and without periosteum. Part I. Histologic evaluation", J Periodontol, 50, pp.586~594 (1979)
- Rambo CR, "Template assisted synthesis of nanofibrous cellulose membranes for tissue engineering", Mater Sci Eng C, 28, pp.549~554 (2008)
- Shulman J, "Clinical evaluation of an acellular dermal allograft for increasing the zone of attached gingiva", Pract Periodontics Aesthet Dent, 8, pp.201~208 (1996)
- Harris RJ, "Root coverage with a connective tissue with partial thickness double pedicle graft and an acellular dermal matrix graft : a clinical and histological evaluation of a case report", J Periodontol, 69, pp.1305~1311 (1998)
- Wei PC, "Acellular dermal matrix allografts to achieve increased attached gingiva. Part 1. A clinical study", J Periodontol, 71, pp.1297~1305 (2000)