가토 하악골 절단부의 저수준 레이저 처치가 골치유에 미치는 영향
BONE HEALING CAPACITY IN THE FRACTURE OF RABBIT MANDIBULAR BONE USING LOW-LEVEL LASER
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The concept of biostimulation of wounds by low-level laser therapy (LLLT) is attracting considerable attention. Although its effect on whole tissues has been studied quite extensively, the biological and cellular mechanisms underlying LLLT have not been clarified. In an experimental radius fracture in rabbits, Tang and Chai reported that LLLT enhanced the activity of red blood cells, macrophages, fibroblasts, chondrocytes, and osteoclasts within the fracture area. The purpose of the present study was to evaluate the effect of LLLT with a GaAlAs diode laser device on bone healing in rabbit mandibular fractures. We use 12 rabbits for this study. All rabbits were fractured mandible angle area using saw in anesthetic condition. In control group(n=6), none treatment was performed at fracture site. In experimental group(n=6), LLLT with a GaAlAs diode laser was radiated at fracture site daily for 7 days. All rabbits were sacrificed at 6 weeks later from performed fracture day. We studied the immunohistochemical staining of CD34 and Vimentin and the histochemical analysis for calcium and phosphorus content. The results were as follows. 1. In the histological and immunohistological staining, after 6week, fibroblasts, osteogenic cells and collgen fibers were observed more in experimental group than in control group. 2. In the histochemical analysis, the amount of calcium and phosphorus contents of the experimental group were more than the control group. From the results obtained, we suggest that the bone healing is stimulated by low-level laser irradiation in bone fractures.
Abstract
The concept of biostimulation of wounds by low-level laser therapy (LLLT) is attracting considerable attention. Although its effect on whole tissues has been studied quite extensively, the biological and cellular mechanisms underlying LLLT have not been clarified. In an experimental radius fracture in rabbits, Tang and Chai reported that LLLT enhanced the activity of red blood cells, macrophages, fibroblasts, chondrocytes, and osteoclasts within the fracture area. The purpose of the present study was to evaluate the effect of LLLT with a GaAlAs diode laser device on bone healing in rabbit mandibular fractures. We use 12 rabbits for this study. All rabbits were fractured mandible angle area using saw in anesthetic condition. In control group(n=6), none treatment was performed at fracture site. In experimental group(n=6), LLLT with a GaAlAs diode laser was radiated at fracture site daily for 7 days. All rabbits were sacrificed at 6 weeks later from performed fracture day. We studied the immunohistochemical staining of CD34 and Vimentin and the histochemical analysis for calcium and phosphorus content. The results were as follows. 1. In the histological and immunohistological staining, after 6week, fibroblasts, osteogenic cells and collgen fibers were observed more in experimental group than in control group. 2. In the histochemical analysis, the amount of calcium and phosphorus contents of the experimental group were more than the control group. From the results obtained, we suggest that the bone healing is stimulated by low-level laser irradiation in bone fractures.
참고문헌 (29)
- Einstein A, "Zur quantem theorie der strahlung", Phys Zeit, 18, pp.121~ (1917)
- Ossoff RH, "Laser surgery in otolaryngology", In Ballenger J (1985)
- Cernavia I, "Laser applications in Dentistry : a review of the literature", Australian Dental Journal, 39, pp.28~32 (1994)
- Walsh LJ, "The Current status of low level laser therapy in dentistry. Part I; Soft tissue applications", Australian Dental Journal, 42, pp.247~254 (1997)
- Mester E, "Clinical results of wound healing stimulation studies with laser and experimental studies of the action mechanism", Laser optoelectronics, pp.119~123 (1976)
- Lievens P, "Effects of laser irradiation on the lymph system and wound healing", Pharmakon, 66, pp.65~71 (1986)
- Pick RM, "Laser in denstry. soft tissue procedure", Dent Clin North Am, (37), pp.281~290 (1993)
- Miller M, "Laser in denstry", J Am Dent Assoc, 124, pp.32~38 (1993)
- Clokie C, "The effects of the He-Ne laser on postsurgical discomfort", J Can Dent Assoc, 52, pp.584~ (1991)
- Kert J, "Low level laser therapy used pre-operatively", Laser News, 4, pp.26~32 (1992)
- Roynesdal AK, "The effect of soft laser applicationon post-operative pain and swelling", Int J Oral Maxillofac Surg, 22, pp.242~251 (1993)
- Bosata M, "In vitro fibroblastdermis fibroblast activation by laser irradiation at low energy", Dermatologic, 168, pp.157~162 (1984)
- Tang XM, "Effect of CO2 laser irradiation on experimental fracture healing; a transmission electron microscopic study", Lasers Surg Med, 6, pp.346~352 (1986)
- Elchanan J, "Effect of low-power laser irradiation on the mechanical properties of bone frecture healing in rats", Laser in Surgery and Medicine, 22, pp.97~102 (1998)
- LJ Miserendino, "Lasers in Dentistry" (1995), Quintessence Publishing Co
- Mona K Marei, "Effect of low-energy laser application in the treatment of dentureinduced mucosal lesions", J prosthet Dent, 77, pp.256~261 (1997)
- Yasunori Takeda, "Irradiation effect of low energy laser on alveolar bone after tooth extraction", Int. J Oral Maxillofac Surg, 17, pp.388~395 (1988)
- Ki-Suk Kim, "Effects of low level laser irradiation with 904 nm pulsed diode laser on the extraction wound", J of Korean Academy of Oral Med, 23, pp.301~309 (1998)
- Masami Akai, "Lasers effect on bone and cartilage change induced by joint immobilization", Laser in Surg andMed, 21, pp.480~488 (1997)
- Maawan K, "Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy", Oral Surg Oral Med Oral Patho., 97, pp.693~700 (2004)
- Lam TS, "Laser stimulation of collagen synthesis in human skin fibroblast culture", LaserLifr Sci., 1, pp.61~77 (1986)
- Oron U, "Attenuation of infarct size in rat and dog after myocardial infarction by lowenergy laser irradiation", Laser Surg Med., 28, pp.204~211 (2001)
- Morimoto Y, "Effect of low intensity Argon laser irradiation on mitrochodria respiration", Laserds Surg Med., 15, pp.191~200 (1994)
- Yu X, "Photomodulation of oxative metabolism and electron chain enzymes in rat liver mitochodria", Photochem Photobiol., 66, pp.866~871 (1997)
- Chen JW, "Effect of low level carbon dioxide laser radiationon biochemical metabolism of rabbit mandibular bone callus", Laser Therapy, 2, pp.83~7 (1989)
- Yoshihide Kobu, "Effects on infrared radiation on intraosseous blood flow and oxygen tension in the rat tibia", Kobe J Med Sci., 45, pp.27~39 (1999)
- Robert F Labbe, "Laser photobioactivation mechanisms", Lasers in Surgery and Medicine, 10, pp.201~210 (1990)
- Tuderman L, "Mechanism of the prolyhydroxylase reaction", Nutr Rev, 40, pp.306~ (1982)
- Trelles M, "Bone fracture consolidates faster with lowpower laser", Lasers Surg Med, 7, pp.36~45 (1986)