상피이형성 발생 마우스 모델에서의 VELscope를 이용한 진단의 유용성 평가
Evaluation of diagnosis using VELscope in mouse model of oral dysplasia
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Intraoral precancerous lesions can be diagnosed early through noninvasive and fast diagnosis using an autofluorescent device. The purpose of this study was to evaluate the usefulness of Visually Enhanced Lesion Scope (VELscope; LED Dental Inc.) as a diagnostic device for oral cancer. Materials and Methods: In this study, 24 6-week-old mice were divided into two groups: six mice in the control group and 18 in the experimental group. Conditions of mices’ tongues were evaluated after developing epithelial dysplasia using 4-nitroquinoline-l-oxide (4NQO, Sigma Aldrich) solution. Clinical photos were taken with Velscope, which diagnoses oral cancer using an autofluorescent device. Histological analysis was conducted every 4, 8, and 12 weeks for comparison with Velscope images. Results: Oral epithelial dysplasia was observed on tongues treated with 4NQO solution for 8 weeks. As time went by, dysplasia progressed and carcinogenesis was observed. The developing grade of dysplasia was moderate grade. Conclusion: Therefore, after dysplasia develops, lesion cloud could be distinguished by visual inspection and Velscope, although it remains to be confirmed whether Velscope is more beneficial than clinical visualization.
Abstract
Purpose: Intraoral precancerous lesions can be diagnosed early through noninvasive and fast diagnosis using an autofluorescent device. The purpose of this study was to evaluate the usefulness of Visually Enhanced Lesion Scope (VELscope; LED Dental Inc.) as a diagnostic device for oral cancer. Materials and Methods: In this study, 24 6-week-old mice were divided into two groups: six mice in the control group and 18 in the experimental group. Conditions of mices’ tongues were evaluated after developing epithelial dysplasia using 4-nitroquinoline-l-oxide (4NQO, Sigma Aldrich) solution. Clinical photos were taken with Velscope, which diagnoses oral cancer using an autofluorescent device. Histological analysis was conducted every 4, 8, and 12 weeks for comparison with Velscope images. Results: Oral epithelial dysplasia was observed on tongues treated with 4NQO solution for 8 weeks. As time went by, dysplasia progressed and carcinogenesis was observed. The developing grade of dysplasia was moderate grade. Conclusion: Therefore, after dysplasia develops, lesion cloud could be distinguished by visual inspection and Velscope, although it remains to be confirmed whether Velscope is more beneficial than clinical visualization.
참고문헌 (21)
- Parkin DM, "Global cancer statistics in the year 2000", Lancet Oncol, 2, pp.533~543 (2001)
- Mignogna MD, "Oral cancer screening: 5 minutes to save a life", Lancet, 365, pp.1905~1906 (2005)
- Thomson PJ, "Field change and oral cancer: new evidence for widespread carcinogenesis?", Int J Oral Maxillofac Surg, 31, pp.262~266 (2002)
- Silverman S Jr, "Early diagnosis of oral cancer", Cancer, 62(8 Suppl), pp.1796~1799 (1988)
- Napier SS, "Natural history of potentially malignant oral lesions and conditions: an overview of the literature", J Oral Pathol Med, 37, pp.1~10 (2008)
- Pavlova I, "Understanding the biological basis of autofluorescence imaging for oral cancer detection: high-resolution fluorescence microscopy in viable tissue", Clin Cancer Res, 14, pp.2396~2404 (2008)
- Westra WH, "Fluorescence visualization in oral neoplasia: shedding light on an old problem", Clin Cancer Res, 12, pp.6594~6597 (2006)
- Schantz SP, "Mutagen sensitivity in patients with head and neck cancers: a biologic marker for risk of multiple primary malignancies", J Natl Cancer Inst, 82, pp.1773~1775 (1990)
- Slaughter DP, "Field cancerization in oral stratied squamous epithelium; clinical implications of multicentric origin", Cancer, 6, pp.963~968 (1953)
- Farber E, "The multistep nature of cancer development", Cancer Res, 44, pp.4217~4223 (1984)
- Salley JJ, "Experimental carcinogenesis in the cheek pouch of the Syrian hamster", J Dent Res, 33, pp.253~262 (1954)
- Fujino H, "Experimental production of labial and lingual carcinoma by local application of 4-nitroquinoline N-oxide", J Natl Cancer Inst, 35, pp.907~918 (1965)
- Wallenius K, "Oral cancer in rats induced by the water-soluble carcinogen 4-nitrochinoline N-oxide", Odontol Revy, 24, pp.39~48 (1973)
- Eveson JW, "Animal models of intra-oral chemical carcino-genesis: a review", J Oral Pathol, 10, pp.129~146 (1981)
- Mirzayans R, "Inverse corre-lation between p53 protein levels and DNA repair eÀciency in human broblast strains treated with 4-nitroquinoline 1-oxide: evidence that lesions other than DNA strand breaks trigger the p53 response", Carcinogenesis, 20, pp.941~946 (1999)
- Fujita K, "Experimental production of lingual carcinomas in hamsters: tumor characteristics and site of formation", J Dent Res, 52, pp.1176~1185 (1973)
- Fujita K, "Experimental production of lingual carcinomas in hamsters by local application of 9, 10-dimethyl-1, 2-benzanthracene", J Dent Res, 52, pp.327~332 (1973)
- Marefat P, "Experimental production of lingual leukoplakia and carcinoma", Oral Surg Oral Med Oral Pathol, 44, pp.578~586 (1977)
- Poh CF, "Fluorescence visualization detection of field alterations in tumor margins of oral cancer patients", Clin Cancer Res, 12, pp.6716~6722 (2006)
- Lane PM, "Simple device for the direct visualization of oral-cavity tissue fluorescence", J Biomed Opt, 11, pp.024006~ (2006)
- McNamara KK, "The role of direct visual fluorescent examination (VELscope) in routine screening for potentially malignant oral mucosal lesions", Oral Surg Oral Med Oral Pathol Oral Radiol, 114, pp.636~643 (2012)