본문 바로가기
KERD
논문KCI 등재

백서 상부 소화기계의 발달 시기별 Telomerase 활성 및 PCNA(proliferating cell nuclear antigen)의 발현 변화

The changes of telomerase activity and proliferating cell nuclear antigen(PCNA) expression in the developmental stages of rat’s upper digestive system

김종렬|성일용
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2004.04|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제30권 제2호|pp. 85-99

0

조회

0

다운로드

0

인용

0

참고문헌

주제분류치의학

초록

Abstract

This study observed the changes in the telomerase activity, it's developmental regulation, PCNA expression, and their correlation in rat's upper digestive organs during growth and aging. Upper digestive organs(buccal mucosa, gingiva, palate, submandibular and parotid glands, and tongue) were aseptically removed from Sprague-Dawley rats of fetal(gestational 20 days), growing(1, 2, 3, 5, and 7 weeks after birth) and adult(12 week old). Samples for telomerase activity were frozen on liquid nitrogen immediately after sacrifice, and stored until the use at -75℃ in order to measure it. telomerase activity was measured by a PCR-based telomeric repeat amplication protoco(TRAP) assay and quantitated with Photometric Telo TAGGG Telomerase PCR ELISA plus(Roche Diagnostics GmbH. Mannheim. Germany). PCNA expression were measured immunohistochemistry with anti PCNA Ab-1, Clone PC10(NeoMark. California. USA). 1. Telomerase activities in buccal mucosa, palate and gingiva were the highest in fetus and decreased gradually or rapidly after birth and then diminished, but in salivary gland and tongue were the highest in fetus and also high at 1 week and then decreased rapidly 2. PCNA expression in buccal mucosa, gingiva, tongue and salivary gland was the highest in fetus and decreased gradually and then diminished. but only in palate decreased rapidly after birth and then diminished. The highest telomerase activity of embryonic stage decreased rapidly after birth in rat‘s upper digestive organs. There may be a developmental regulation of telomerase activity, but not a tissue-specific. This telomerase activity seems correlated closely with PCNA expression in rat's upper digestive system.

참고문헌 (28)

  1. "The telomere terminal transferase of tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity", 51, pp.887~98 (1987)
  2. "The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats", 59, pp.521~9 (1989)
  3. "Telomerase activity in human germline and embryonic tissues and cells", 18, pp.173~179 (1996)
  4. "Telomeres and telomerase in aging and cancer", 5, pp.249~255 (1995)
  5. "Tolmerase activity in head and neck squamous cell carcinoma and adjacent tissues", 56, pp.5600~5604 (1996)
  6. "Telomerase activity in oral leukoplakia and head and neck squamous cell carcinoma", 56, pp.3530~3533 (1996)
  7. "Advances in quantification and characterization of telomerase activity by the telomeric repeat amplication protocol", 25, pp.2595~2597 (1997)
  8. "Specific association of human telomerase activity with immortal cells and cancer", 266, pp.2011~5 (1994)
  9. "Telomeres, telomerases, and myc.", 462, pp.31~47 (2000)
  10. "Changes in cyclin", PCNA distribution during DNA repair synthesis, 1988, pp.107, 1623~8
  11. "and DNA index in human tumors", pp.586~95 (1988)
  12. "Flow cytometric multiparameter analysis of proliferating cell nuclear antigen", cyclin and Ki-67 antigen, 1990, pp.187, 111~8
  13. "Comparison of five histopathologic methods to assess cellular proliferation in transitional cell carcinoma of the urinary bladder", pp.411~86 (1993)
  14. "Change in telomerase of rat organs during growth and aging", 242, pp.120~127 (1998)
  15. "Developmental and tissue -specific regulation of mouse telomerase and telomere length", 92, pp.4818~4822 (1995)
  16. "Functional characterization and developmental regulation of mouse telomerase RNA", 269, pp.1267~1270 (1995)
  17. "Selective inhibition of telomerase activity during terminal differentiation of immortal cell lines", 56, pp.3796~3802 (1996)
  18. "Structure and function of telomeres", 350, pp.569~573 (1991)
  19. "Telomere", pp.363~9
  20. "Structure and function of telomeres", 23, pp.579~604 (1989)
  21. "Telomerase activity in premalignant and lesions of human oral mucosa", 6, pp.413~420 (1997)
  22. "Detection of telomerase activity in oral lesions", 27, pp.111~115 (1998)
  23. "Telomerase activity in head and neck squamous cell carcinoma and adjacent tissues", 56, pp.5600~5604 (1996)
  24. "Telomerase activity in normal human epithelial cells", 13, pp.433~439 (1996)
  25. "Telomerase activity in esophageal carcinoma", 66, pp.88~92 (1997)
  26. "Telomerase activity is associated with cell cycle deregulation in human breast cancer", 57, pp.549~554 (1997)
  27. "Telomerase activity in oral and maxillofacial tumors", 36, pp.347~352 (2000)
  28. "백서 신장의 발달 시기별 Telomerase 활성 및 Proliferating Cell Nuclear Antigen(PCNA)발현에 관한 연구", 41, pp.1041~1050 (2000)

관련 논문AI추천

닭의 발생 단계별 세포내 Telomere의 양적 분포양상과 Telomerase 활성도 분석손시환, 조은정, 최철환한국축산학회 · 한국축산학회지2005 · 제47권 제2호흰쥐의 위십이지장에서 방사선 조사에 의하여 유발된 Apoptosis와 세포증식능의 변화민병운, 황구연대한임상검사과학회 · 대한임상검사과학회지2003 · 제35권 제2호Expressional Profiling of Telomerase and Telomere-Associated Molecules in the Rat Testis and Seminal Vesicle during Postnatal Developmental Period백행운, 서희정, 이기호, 이성규, 전용필, 전태훈, 최인호한국축산학회 · 한국축산학회지2011 · 제53권 제3호소의 생리적 특성에 따른 세포내 텔로미어 함량과 텔로머레이스 활성도 분석손시환, 조창연, 최덕순한국축산학회 · 한국축산학회지2008 · 제50권 제4호자궁내 발달지연과 정상 말기임신 태반에서의 Telomerase 활성도와 MIB-1 및 Fas, Fas Ligand 발현차이에 관한 연구양문호, 전이경, 홍성란대한병리학회 · Journal of Pathology and Translational Medicine2005 · 제39권 제1호Urethane에 의한 생쥐의 폐암 발생 과정에서 Telomerase의 활성도박광화, 송지선, 오화은, 이상엽, 정순희, 조미연대한병리학회 · Journal of Pathology and Translational Medicine2011 · 제45권 제3호

이 간행물의 다른 논문 (11건)

구강편평세포암종 이식 누드마우스에서의 혈액 점도 변화이종호, 김명진, 명훈, 정필훈pp. 81-84신생 백서 척수후근절의 슈반세포 배양을 위한 Ara-C 분열억제제의최적 효과에 대한 연구김남열, 김지혁, 이종호, 김성민, 성미애, 황순정, 장정원, 안강민pp. 100-107하악골 전돌증을 동반한 안모비대칭의 유형 분석강군철, 박형식, 정영수, 윤규식pp. 108-120성장기 아동 하악 과두 골절의 예후에 관한 장기 추적 조사최진호, 류문광, 류승현, 김일규, 하태진, 김재우pp. 121-130하악전돌증 환자에서 내측 익돌근 근력측정 및 분석홍종락, 정필훈, 강나라pp. 131-135
원문 보기