본문 바로가기
KERD
논문

소의 생리적 특성에 따른 세포내 텔로미어 함량과 텔로머레이스 활성도 분석

The Amount of Telomeric DNA and Telomerase Activity on Cattle Cells

조창연|손시환|최덕순
한국축산학회 (Korean Society of Animal Sciences and Technology)|2008.08|한국축산학회지 (Journal of Animal Science and Technology)|제50권 제4호|pp. 445-456

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

CattleTRAPTelomeraseTelomereQ-FISH
주제분류축산학

초록

Telomeres consist of TTAGGG tandem repeated DNA sequences with specific proteins and locate at chromosome ends. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Telomerase is a ribonucleoprotein which has a template for the synthesis of telomeric DNA. This study was carried out to analyze the amount of telomeric DNA and telomerase activity in cattle cells. Analysis of the quantity of telomere in lymphocytes was done at different ages, sex and among Korean cattle and Holstein breeds. The telomerase activity was also analyzed in liver, brain, heart, kidney, and testis tissues of fetal calf and of 18 month old cattle. The amount of telomeres in lymphocytes and other tissue cells was analyzed by Quantitative-Fluorescence in situ Hybridization (Q-FISH) technique using a telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol assay (TRAP). The amount of telomeric DNA on the lymphocytes during the whole life span was decreased along with age. Quantity of telomeres in Korean cattle was significantly higher than that in Holstein breed. The amount of telomeric DNA in males was significantly higher than that in females. Telomerase activity was up-regulated in most bovine tissues during fetal stage, but was down-regulated in most tissues at mature 18 month age except the testis cells. This study indicates that the amount of telomeres and telomerase activity of cells can be used as an age marker or/and a physiological marker of cattle.

Abstract

Telomeres consist of TTAGGG tandem repeated DNA sequences with specific proteins and locate at chromosome ends. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Telomerase is a ribonucleoprotein which has a template for the synthesis of telomeric DNA. This study was carried out to analyze the amount of telomeric DNA and telomerase activity in cattle cells. Analysis of the quantity of telomere in lymphocytes was done at different ages, sex and among Korean cattle and Holstein breeds. The telomerase activity was also analyzed in liver, brain, heart, kidney, and testis tissues of fetal calf and of 18 month old cattle. The amount of telomeres in lymphocytes and other tissue cells was analyzed by Quantitative-Fluorescence in situ Hybridization (Q-FISH) technique using a telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol assay (TRAP). The amount of telomeric DNA on the lymphocytes during the whole life span was decreased along with age. Quantity of telomeres in Korean cattle was significantly higher than that in Holstein breed. The amount of telomeric DNA in males was significantly higher than that in females. Telomerase activity was up-regulated in most bovine tissues during fetal stage, but was down-regulated in most tissues at mature 18 month age except the testis cells. This study indicates that the amount of telomeres and telomerase activity of cells can be used as an age marker or/and a physiological marker of cattle.

참고문헌 (38)

  1. Ahmed, A, "Telomeres and telomerase: basic science implications for aging", J. Am. Geriatr. Soc, 49(8), pp.1105~1109 (2001)
  2. Benetos, A., "Telomere length as an indicator of biological aging: The gender effect and relation with pulse pressure and pulse wave velocity", Hypertension, 37(2), pp.381~385 (2001)
  3. Betts, D. H, "Telomerase activity and telomere detection during early bovin development", Dev Gent, 25(4), pp.397~403 (1999)
  4. Betts, D. H., "Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle", Proc. Natl. Sci. USA, 통권 : 98, pp.1077~1082 (2001)
  5. Bradford, M. M, "A rapid and sensitive method for the quantitation of microgram quanti- ties of protein utilizing the principle of protein- dye binding", Animal Biochem, 72, pp.248~254 (1976)
  6. Cottliar, A. S, "Telomeres and telomerase activity: their role in aging and in neoplastic development", Medicina, 61, pp.335~342 (2001)
  7. de la Sena, C., "Localization of telomeric(TTAGGG)n sequences in chromosomes of some domestic animals by fluorescence in situ hybridization", Hereditas, 123, pp.269~274 (1995)
  8. Frenck, R. W. Jr., "The rate of telomere sequence loss in human leukocytes varies with age", Proc Natl Acad Sci USA, 95(10), pp.5607~5610 (1998)
  9. Gan, Y., "Telomere amount and length assay", Pharma. Res, 18(12), pp.1655~1659 (2001)
  10. Greider, C. W, "Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.Cell 43:405-413", Cell, 43, pp.405~413 (1985)
  11. Hemann, M. T., "The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability", Cell, 107, pp.67~77 (2001)
  12. Kim, N. W, "Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol(TRAP)", Nucleic Acids Research, 25(13), pp.2595~2597 (1997)
  13. King, W. A., "Telomeres and telomerase activity in bovine oocytes, embryos and fetuses", Theriogenology, 49(1), pp.182~ (1998)
  14. Lustig, A. J, "Crisis intervention: the role of telomerase", Proc Natl Acad Sci USA, 96(7), pp.3339~3341 (1999)
  15. Meeker, A. K, "Telomerase: a promising marker of biological immortality of germ, stem, and cancer cells. A review", Biochemistry, 62(11), pp.1323~1331 (1997)
  16. Miyashita, N., "Remarkable difference in telomere lengths among cloned cattle derived from different cell types", Biol. Reprod, 66, pp.1649~1655 (2002)
  17. Okuda, K., "Telomere length in the newborn", Pediatr Res, 52(3), pp.377~381 (2002)
  18. Pathak, S, "Telomere dynamics, aneuploidy, stem cells and cancer", Int. J. Oncology, 20(3), pp.637~641 (2002)
  19. Perner, S., "Quantifying quantitative telomere lengths of human individual chromosome arms by centromere-calibrated fluorescence in situ hybridiz- ation and digital imaging", American Journal of Pathology, 163(5), pp.1751~1756 (2003)
  20. Robinson, M. O, "Telomerase and cancer", Genet. Eng, 22, pp.209~222 (2000)
  21. Samper, E., "Restoration of telomerase activity rescues chromosomal instability and premature aging in Terc-", - mice with short telomeres, 2001, pp.2(9), 800~807
  22. Shiels, P. G., "Analysis of telomere lengths in cloned sheep", Nature, 399, pp.316~317 (1999)
  23. Slijepcevic, P, "Telomere length regulation: a view from the individual chromosome perspective", Exp Cell Res, 244(1), pp.268~274 (1998)
  24. Sohn, S. H., "Telomere erosion-induced mitotic catastrophe in continuously grown Chinese hamster Don cells", Exptl. Cell Res, 279(2), pp.271~276 (2002)
  25. Taylor, H. A, "Ontogeny of telomerase in chicken: impact of downregulation on pre- and postnatal telomere length in vivo", Dev Growth Differ, 42(6), pp.613~621 (2000)
  26. Tian, X. C., "Normal telomere lengths found in cloned cattle", Nat. Genet, 26, pp.272~273 (2000)
  27. Ulaner, G. A, "Develop- mental regulation of telomerase activity in human fetal tissues during gestation", Mol. Hum. Reprod, 3(9), pp.769~773 (1997)
  28. Venkatesan, R, "Telomerase expression in chickens: Constitutive activity in somatic tissues and down-regulation in culture", Proc. Natl Acad. Sci. USA, 통권 : 95, pp.14763~14768 (1998)
  29. Weng, N. P, "The role of telomerase exprerssion and telomere length maintenance in human and mouse", Journal of Clinical Immunology, 20(4), pp.257~267 (2000)
  30. Wilmut, I., "Viable offspring derived from fetal and adult mammalian cells", Nature, 385, pp.810~813 (1997)
  31. Wright, W. E., "Telomerase activity in human germline and embryonic tissues and cells", Development Genetics, 18(2), pp.173~179 (1996)
  32. Xu, J, "Telomerase activity in bovine embryos during early development", Biol Reprod, 63(4), pp.1124~1128 (2000)
  33. Xu, J, "Telomerase activity in early bovine embryos derived from parthenogenetic activation and nuclear transfer", Biol Reprod, 64(3), pp.770~774 (2001)
  34. Yamaguchi, Y., "Change in telomerase activity of rat organs during growth and aging", Exptl. Cell Res, 242, pp.120~127 (1998)
  35. 손시환, "소, 돼지 염색체의 telomeric DNA 분포 양상", 한국동물자원과학회지, 46(4), pp.547~554 (2004), 한국동물자원과학회
  36. 정길선, "한국재래닭의 주령별 각 조직의 텔로미어 양적분포 양상과 텔로머레이스 활성도 분석", 한국가금학회지, 33(2), pp.97~103 (2006)
  37. 조은정, "닭의 발생 단계별 세포내 Telomere의 양적 분포양상과 Telomerase 활성도 분석", 한국동물자원과학회지, 47(2), pp.187~194 (2005), 한국동물자원과학회
  38. 조은정, "Telomere 양적 분석을 이용한 bio-marker 개발", 한국가금학회 제 21차 학술발표회 proceedings, pp.13~15 (2004)

관련 논문AI추천

혈액세포의 텔로미어 함량을 이용한 소의 연령예측김현섭, 손시환, 전광주, 최나은, 최창용한국축산학회 · 한국축산학회지2010 · 제52권 제5호닭의 발생 단계별 세포내 Telomere의 양적 분포양상과 Telomerase 활성도 분석손시환, 조은정, 최철환한국축산학회 · 한국축산학회지2005 · 제47권 제2호양적형광접합보인법(Q-FISH)에 의한 돼지 백혈구 세포의 텔로미어 함량 분석손시환, 정현진, 최덕순한국축산학회 · 한국축산학회지2008 · 제50권 제4호소, 돼지 염색체의 telomeric DNA 분포 양상손시환, 조은정, 하해봉, A. S. Multani, S. Pathak한국축산학회 · 한국축산학회지2004 · 제46권 제4호Expressional Profiling of Telomerase and Telomere-Associated Molecules in the Rat Testis and Seminal Vesicle during Postnatal Developmental Period백행운, 서희정, 이기호, 이성규, 전용필, 전태훈, 최인호한국축산학회 · 한국축산학회지2011 · 제53권 제3호신장 기능과 틸로미어서동철대한소아신장학회 · Childhood Kidney Diseases2008 · 제12권 제1호

이 간행물의 다른 논문 (14건)

돼지의 마이코플라즈마성 폐렴과 경제형질간의 상관관계 연구전병국, 박병석, 이준헌, 김나래, 이종관, 이하복, 오형길, 유임종pp. 437-444돼지 번식형질의 산차간 유전상관조규호, 김인철, 이일주, 전광주, 김명직pp. 457-464양적형광접합보인법(Q-FISH)에 의한 돼지 백혈구 세포의 텔로미어 함량 분석정현진, 최덕순, 손시환pp. 465-474Retinoic Acid가 돼지 지방전구세포의 분화와 유전자 발현에 미치는 영향Baatartsogt Oyungerel, 최영숙, 최강덕, 정정수, 임희경pp. 475-484사료내 희토 첨가가 비육돈의 생산성, 혈액내 면역관련 세포수, 육질특성 및 분 중 악취방출 가스함량에 미치는 영향신승오, 진영걸, 유종상, 이제현, 황염, 장해동, 조진호, 김효진, 김인호pp. 485-498
원문 보기