The BMPs expression and histomorphometric study of β-TCP / rhBMP-2 Grafting on the rabbit cranial bone defects
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The BMPs expression and histomorphometric study of β-TCP /rhBMP-2 Grafting on the rabbit cranial bone defects Byung-Sup Lim, Jae-Yoon Jeon, Chang-Joo Park, Jae-Jung Im, Kyung-Gyun Hwang, Kwang-Sup Shim Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University, Korea * Corresponding author Kyung-Gyun Hwang, DDS, MSD, PhD. Dept. of Dentistry/OMFS, College of Medicine, Hanyang Univ. E-mail: hkg@hanyang.ac.kr Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and β-TCP(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2. Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with 150~500μm diameter β-TCP 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only β-TCP was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. Result: Grafting β-TCP with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of β-TCP (8,12 week), even show less new bone formation than autogenous bone. Conclusion : The experimental study result that β-TCP graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.
Abstract
The BMPs expression and histomorphometric study of β-TCP /rhBMP-2 Grafting on the rabbit cranial bone defects Byung-Sup Lim, Jae-Yoon Jeon, Chang-Joo Park, Jae-Jung Im, Kyung-Gyun Hwang, Kwang-Sup Shim Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University, Korea * Corresponding author Kyung-Gyun Hwang, DDS, MSD, PhD. Dept. of Dentistry/OMFS, College of Medicine, Hanyang Univ. E-mail: hkg@hanyang.ac.kr Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and β-TCP(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2. Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with 150~500μm diameter β-TCP 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only β-TCP was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. Result: Grafting β-TCP with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of β-TCP (8,12 week), even show less new bone formation than autogenous bone. Conclusion : The experimental study result that β-TCP graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.
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