생쥐 두개골에서 동종골 이식 시 면역억제에 대한 cyclosporine A의 효과
The immunosuppression effect of cyclosporine A on the allogenic calvarial bone graft in mice
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Introduction: This study examined the effect of cyclosporine A (CsA) on the allogenic cranial bone graft in the mice. Materials and Methods:Twenty eight 12-week-old male ICR mice weighing 40 g were used. The experimental group was injected subcutaneously with CsA (10 mg/kg/day) diluted in Caster oil for 7 days prior to the graft until sacrifice. The control group was injected with the same solution without CsA. Two full-thickness bone defects with a diameter of 3 mm were made with a trephine bur in the parietal bone lateral to the sagittal suture. A calvarial defect of a mouse was grafted with allogenic calvarial bone disc from another mouse. The experimental and control groups were injected with CsA and the solution without CsA in the same manner before surgery, respectively. The mice were sacrificed at 1 week, 2 weeks and 4 weeks after the bone graft, respectively. Results: In the experimental group, fibrous connective tissues and small amounts of inflammatory cells were observed. At 2 weeks after the allograft in the experimental group, new bone formation in fibrous collagenous tissue and around the allogenic bone was noted. At 4 weeks after the allograft,new bone formation was active along and at the periphery of the mature allogenic bone. The proliferation of blood vessels increased in bone marrow. In the control group, fibrous tissues and inflammatory cells were observed around the allogenic bone and existing bone at 1 week. At 2 weeks after the allograft, the proliferation of blood vessels accompanied by inflammatory cells were scattered in the fibrous connective tissues. New bone formation around the allogenic and existing bone could be observed. At 4 weeks after the allograft, inflammatory cells were severely infiltrated around the allogenic bone. Osteoclasts were scattered along the allogenic bone and induced bone resorption. Conclusion: These results suggest that the daily administration of CsA (10 mg/kg/day) induces efficient immunosuppression without serious complications ,and this protocol might be useful for the experimental model of allogenic bone grafts.
Abstract
Introduction: This study examined the effect of cyclosporine A (CsA) on the allogenic cranial bone graft in the mice. Materials and Methods:Twenty eight 12-week-old male ICR mice weighing 40 g were used. The experimental group was injected subcutaneously with CsA (10 mg/kg/day) diluted in Caster oil for 7 days prior to the graft until sacrifice. The control group was injected with the same solution without CsA. Two full-thickness bone defects with a diameter of 3 mm were made with a trephine bur in the parietal bone lateral to the sagittal suture. A calvarial defect of a mouse was grafted with allogenic calvarial bone disc from another mouse. The experimental and control groups were injected with CsA and the solution without CsA in the same manner before surgery, respectively. The mice were sacrificed at 1 week, 2 weeks and 4 weeks after the bone graft, respectively. Results: In the experimental group, fibrous connective tissues and small amounts of inflammatory cells were observed. At 2 weeks after the allograft in the experimental group, new bone formation in fibrous collagenous tissue and around the allogenic bone was noted. At 4 weeks after the allograft,new bone formation was active along and at the periphery of the mature allogenic bone. The proliferation of blood vessels increased in bone marrow. In the control group, fibrous tissues and inflammatory cells were observed around the allogenic bone and existing bone at 1 week. At 2 weeks after the allograft, the proliferation of blood vessels accompanied by inflammatory cells were scattered in the fibrous connective tissues. New bone formation around the allogenic and existing bone could be observed. At 4 weeks after the allograft, inflammatory cells were severely infiltrated around the allogenic bone. Osteoclasts were scattered along the allogenic bone and induced bone resorption. Conclusion: These results suggest that the daily administration of CsA (10 mg/kg/day) induces efficient immunosuppression without serious complications ,and this protocol might be useful for the experimental model of allogenic bone grafts.
참고문헌 (27)
- Yanagihara RH, "Inhibition of mouse natural killer activity by cyclosporin A", Immunology, 45, pp.325~332 (1982)
- Wachowiak J, "Effects of cyclosporin A on the activity of mouse natural killer cells and hybrid resistance", Immunol Lett, 13, pp.95~99 (1986)
- Yanagihara RH, "Cyclosporin a inhibits T cell-mediated augmentation of mouse natural killer activity", Immunopharmacology, 4, pp.243~252 (1982)
- Nemlander A, "Effect of cyclosporin A on the generation of cytotoxic T lymphocytes in mouse mixed lymphocyte culture", Scand J Immunol, 12, pp.493~498 (1980)
- Alberti S, "Effects of in vivo treatments with cyclosporin-A on mouse cell-mediated immune responses", Int J Immunopharmacol, 3, pp.357~364 (1981)
- Friedlaender GE, "Immune responses to osteochondral allografts. Current knowledge and future directions", Clin Orthop Relat Res, (174), pp.58~68 (1983)
- Friedlaender GE, "Bone allografts: the biological consequences of immunological events", J Bone Joint Surg Am, 73, pp.1119~1122 (1991)
- Horowitz MC, "Immunologic aspects of bone transplantation. A rationale for future studies", Orthop Clin North Am, 18, pp.227~233 (1987)
- Horowitz MC, "Induction of specific T-cell responsiveness to allogeneic bone", J Bone Joint Surg Am, 73, pp.1157~1168 (1991)
- Stevenson S, "The immune response to osteochondral allografts in dogs", J Bone Joint Surg Am, 69, pp.573~582 (1987)
- Stevenson S, "The response to bone allografts", J Bone Joint Surg Am, 74, pp.939~950 (1992)
- Ekelund AL, "Effects of cyclosporin A on bone turnover and on resorption of demineralized bone matrix", Clin Orthop Relat Res, (326), pp.127~134 (1996)
- Frame JW, "A convenient animal model for testing bone substitute materials", J Oral Surg, 38, pp.176~180 (1980)
- Casser-Bette M, "Bone formation by osteoblast-like cells in a three-dimensional cell culture", Calcif Tissue Int, 46, pp.46~56 (1990)
- Bhargava U, "Ultrastructural analysis of bone nodules formed in vitro by isolated fetal rat calvaria cells", Bone, 9, pp.155~163 (1988)
- in association with mineralized-tissue formation by fetal-rat calvarial cells in culture, "Biochem J", 1991, 513~520
- Ellis CN, "Cyclosporine for plaque-type psoriasis. Results of a multidose, double-blind trial", N Engl J Med, 324, pp.277~284 (1991)
- Green CJ, "Experimental transplantation and cyclosporine", Transplantation, 46(2), pp.3~10 (1988)
- Tugwell P, "Low-dose cyclosporin versus placebo in patients with rheumatoid arthritis", Lancet, 335, pp.1051~1055 (1990)
- Boland J, "Tissue distribution and toxicity of cyclosporin A in the mouse", Pathology, 16, pp.117~123 (1984)
- Shi CL, "Effects of cyclosporin A and verapamil on mouse pancreatic islets", Acta Endocrinol (Copenh), 129, pp.54~58 (1993)
- Fabien NH, "Effects of cyclosporin A on mouse thymus: immunochemical and ultrastructural studies", Thymus, 20, pp.153~162 (1992)
- Aramant R, "Cross-species grafting of embryonic mouse and grafting of older postnatal rat retinas into the lesioned adult rat eye: the importance of cyclosporin A for survival", Brain Res, 469, pp.303~307 (1988)
- Lems SP, "Rejection of long-surviving mouse skin allografts after withdrawal of cyclosporin A therapy", Transplant Proc, 12, pp.283~286 (1980)
- Autenried P, "Cyclosporine blocks the induction of class I and class IIMHC products in mouse kidney by graft-vshost disease", J Immunol, 135, pp.3922~3928 (1985)
- Halloran PF, "Orthotopic bone transplantation in mice. II. Studies of the alloantibody response", Transplantation, 27, pp.420~426 (1979)
- Urist MR, "The bone induction principle", Clin Orthop Relat Res, 53, pp.243~283 (1967)