오존수를 이용한 내시경 자동 세척기의 소독 효과
Disinfection Efficacy of an Automated Endoscopic Reprocessing System withOzonated Water
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목 적 : 내시경 소독제 중 하나로 최근 오존수가 제시되고 있으나 국내외를 통틀어 그 연구 자료가 매우 부족하다. 이에 저자들은 오존수를 이용한 내시경 자동 세척기의 소독 효과를 알아보기로 하였다. 재료 및 방법 : 최근 구입한 내시경 한 개만을 사용하여 진단적 상부위장관 내시경을 시행 받은 환자들 중 30명을 내시경 검사 시행 후 내시경 소독법에 따라 무작위적으로 각 10명씩 3군으로 나누었다(A군, 오존수 5분 소독; B군, 오존수 10분 소독; C군, 전해산성수 3분 30초 소독). 결 과 : 배양 양성률에 따라 무배양군(0 CFU/mL)과 배양군(>0 CFU/mL)으로, 배양 집락수에 따라 저배양군(<1 CFU/mL)과 고배양군(≤1 CFU/mL)으로 분류하였다. 내시경 선단부에서 얻은 검체의 배양 양성률은 3군 모두 0%였다. 생검겸자관 공에서 얻은 검체의 배양 양성률은 A, B, C군이 각각 70% (7/10), 70% (7/10), 90% (9/10)이었다, 배양 집락수에 따라서는 A군에서는 고배양군에 속한 경우가 없었으나, B군에서는 30% (3/10), C군에서는 70% (7/10)가 고배양군에 속했으며, A군과 C군 사이에서만 고배양률에서 유의한 차이가 나타났다(P<0.05).
Abstract
Background : The aim of this study is to evaluate the effectiveness of automated ozonated water endoscopic reprocessing system (AORS). Materials and Methods : Thirty cases were collected and randomly assigned to 3 groups according to the disinfection methods (Group A, AORS for 5 minutes; Group B, AORS for 10 minutes; Group C, automated disinfection with superoxidized water for 3 minutes 30 seconds). After disinfection was finished, samples were collected from the tip of scopes (Site 1, S1) and rinsing water through biopsy channel (Site 2, S2). Samples were inoculated in blood agar plate for 48 hrs, and then colony count was evaluated. Results : Culture positive rate of S1 was 0% in all three groups. Culture positive rates of S2 were 70% (7/10), 70% (7/10) and 90% (9/10) in group A, group B and group C, respectively. High culture rate group (■1 CFU/ml rinsing water) was 0% (0/10), 30% (3/10) and 70% (7/10) in group A, group B and group C, respectively. Disinfection efficacy between group A and C showed a significant difference in high culture rate (P<0.05). Conclusions : AORS for 5min was at least equally effective in endoscopic reprocessing compared with the conventional superoxidized water system.
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