Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method
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A multiplex PCR method has been developed to classify extended spectrum β-lactamase (ESBL) and plasmid- mediated AmpC β-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type β-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL- producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of β-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
Abstract
A multiplex PCR method has been developed to classify extended spectrum β-lactamase (ESBL) and plasmid- mediated AmpC β-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type β-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL- producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of β-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
참고문헌 (11)
- Bradford PA, "Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat", Clin Microbiol Rev, 14, pp.933~951 (2001)
- Bush K, "New beta-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy", Clin Infect Dis, 32, pp.1085~1089 (2001)
- Pai H, "Epidemiology and clinical features of bloodstream infections caused by AmpC-type-beta-lactamases-producing Klebsiella pneumoniae", Antimicrob Agents Chemother, 48, pp.3720~3728 (2004)
- Pai H, "High prevalence of extended-spectrum beta-lactamases- producing strains among blood isolates of Enterobacter spp. collected in a tertiary hospital during an 8-year period and their antimicrobial susceptibility patterns", Antimicrob Agents Chemother, 48, pp.3159~3161 (2004)
- Shah AA, "Characteristics, epidemiology and clinical importance of emerging strains of Gram-negative bacilli producing extended-spectrum beta-lactamases", Res Microbiol, 155, pp.409~421 (2004)
- Lee J, "Control of extended-spectrum beta-lactamase- producing Escherichia coli and Klebsiella pneumoniae in a children’s hospital by changing antimicrobial agent usage policy", J Antimicrob Chemother, 60, pp.629~637 (2007)
- Kim S, "Occurrence of extended-spectrum-beta-lactamases in members of the genus Shigella in the Republic of Korea", J Clin Microbiol, 42, pp.5264~5269 (2004)
- Bonnet R, "Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes", Antimicrob Agents Chemother, 48, pp.1~14 (2004)
- Pitout JD, "Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp", J Clin Microbiol, 42, pp.5715~5721 (2004)
- "Amino acid sequences for TEM, SHV and OXA extended-spectrum and inhibitor-resistant β-lactamases"[URL]
- Babini GS, "Are SHV beta-lactamases univesal in Klebsiella pneumoniae? Antimicrob Agents Chemother 44:2230, 2000", ~