본문 바로가기
KERD
논문KCI 등재

Frequency of Extended-spectrum b-lactamase (ESBL) and AmpC b-lactamase Genes in Escherichia coli and Klebsiella pneumoniae over a Three-year Period in a University Hospital in Korea

박영진|김영숙|박건|신종희|장숙진|배일권|문대수|이학민
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2010.12|Annals of Laboratory Medicine|제30권 제6호|pp. 616-623

1

조회

0

다운로드

12

인용

0

참고문헌

키워드

PrevalenceEscherichia colib-lactamaseKlebsiella pneumonia
주제분류병리학

초록

Background : The aim of this study was to determine the yearly prevalence and genotype distribution of extended-spectrum b-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae collected over a 3-yr period in Gwangju, Korea. Methods : Clinical isolates of E. coli and K. pneumoniae collected at Chosun University Hospital from September 15, 2005 to September 14, 2008 were evaluated. Antimicrobial susceptibility testing was performed using the Vitek II system (bioMe@rieux, USA) and agar dilution methods. Screening for ESBL and AmpC b-lactamase genes was performed using PCR amplification of plasmid DNA followed by direct sequencing of the PCR products. Results : The percentage of ESBL-producing isolates was 12.6% (196/1,550) for E. coli and 26.2% (294/1,121) for K. pneumoniae. The ESBL gene sequencing results showed that the most prevalent ESBL types were CTX-M (93.5%) and SHV (12.9%) in E. coli, and SHV (73.2%) and CTXM (46.3%) in K. pneumoniae. The most common ESBL in E. coli was CTX-M-15-like, followed by CTX-M-14-like, SHV-2a-like, and SHV-12-like. The most prevalent ESBL type in K. pneumoniae was SHV-12, followed by CTX-M-14-like and CTX-M-15-like. Fifty-one percent (21/41) of ESBL-producing K. pneumoniae with ESBL types verified by sequencing also had DHA-1-like AmpC b-lactamases. However, none of the ESBL-producing E. coli was positive in the AmpC b-lactamase PCR analysis. Conclusions : In this study, the most common types of class A ESBLs identified were CTX-M-15-like in E. coli and SHV-12-like in K. pneumoniae. (Korean J Lab Med 2010;30:616-23)

Abstract

Background : The aim of this study was to determine the yearly prevalence and genotype distribution of extended-spectrum b-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae collected over a 3-yr period in Gwangju, Korea. Methods : Clinical isolates of E. coli and K. pneumoniae collected at Chosun University Hospital from September 15, 2005 to September 14, 2008 were evaluated. Antimicrobial susceptibility testing was performed using the Vitek II system (bioMe@rieux, USA) and agar dilution methods. Screening for ESBL and AmpC b-lactamase genes was performed using PCR amplification of plasmid DNA followed by direct sequencing of the PCR products. Results : The percentage of ESBL-producing isolates was 12.6% (196/1,550) for E. coli and 26.2% (294/1,121) for K. pneumoniae. The ESBL gene sequencing results showed that the most prevalent ESBL types were CTX-M (93.5%) and SHV (12.9%) in E. coli, and SHV (73.2%) and CTXM (46.3%) in K. pneumoniae. The most common ESBL in E. coli was CTX-M-15-like, followed by CTX-M-14-like, SHV-2a-like, and SHV-12-like. The most prevalent ESBL type in K. pneumoniae was SHV-12, followed by CTX-M-14-like and CTX-M-15-like. Fifty-one percent (21/41) of ESBL-producing K. pneumoniae with ESBL types verified by sequencing also had DHA-1-like AmpC b-lactamases. However, none of the ESBL-producing E. coli was positive in the AmpC b-lactamase PCR analysis. Conclusions : In this study, the most common types of class A ESBLs identified were CTX-M-15-like in E. coli and SHV-12-like in K. pneumoniae. (Korean J Lab Med 2010;30:616-23)

참고문헌 (20)

  1. Jong Hwa Yum, "Emergence and Wide Dissemination of CTX-M-type ESBLs, andCMY-2- and DHA-1-type AmpC -Lactamases in Korean RespiratoryIsolates of Klebsiella pneumoniae", Journal of Korean Medical Science, 20(6), pp.961~965 (2005), 대한의학회
  2. Lee KM, "Clonal spread of both oxyimino-cephalosporin- and cefoxitin-resistant Klebsiella pneumoniae isolates co-producing SHV-2a and DHA-1 b-lactamase at a burns intensive care unit", Int J Antimicrob Agents, 28, pp.520~524 (2006)
  3. National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement, M100-S12", National Committee for Clinical Laboratory Standards, ~ (2002)
  4. Jeong SH, "Investigation of extended-spectrum b-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea", Lett Appl Microbiol, 39, pp.41~47 (2004)
  5. Saladin M, "Diversity of CTX-M b-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals", FEMS Microbiol Lett, 209, pp.161~168 (2002)
  6. Jeong SH, "Dissemination of transferable CTX-M-type extended-spectrum b-lactamase-producing Escherichia coli in Korea", J Appl Microbiol, 98, pp.921~927 (2005)
  7. Machado E, "Integron content of extended-spectrum-b-lactamase-producing Escherichia coli strains over 12 years in a single hospital in Madrid, Spain", Antimicrob Agents Chemother, 49, pp.1823~1829 (2005)
  8. Ryoo NH, "Dissemination of SHV-12 and CTX-M-type extended-spectrum b-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea", J Antimicrob Chemother, 56, pp.698~702 (2005)
  9. Pe@rez-Pe@rez FJ, "Detection of plasmid-mediated AmpC b-lactamase genes in clinical isolates by using multiplex PCR", J Clin Microbiol, 40, pp.2153~2162 (2002)
  10. Ko KS, "Prevalence and characterization of extended-spectrum b-lactamase-producing Enterobacteriaceae isolated in Korean hospitals", Diagn Microbiol Infect Dis, 61, pp.453~459 (2008)
  11. Song W, "CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum b-lactamase in clinical isolates of Escherichia coli from Korea", J Med Microbiol, 58, pp.261~266 (2009)
  12. Kim J, "CTX-M and SHV-12 b-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 3 university hospitals within Korea", FEMS Microbiol Lett, 245, pp.93~98 (2005)
  13. Jeong SH, "Molecular characterization of extended-spectrum b-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey", J Clin Microbiol, 42, pp.2902~2906 (2004)
  14. 박용정, "Prevalence of the Extended-Spectrum β-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli", The Korean Journal of Laboratory Medicine, 29(3), pp.218~223 (2009), 대한진단검사의학회
  15. Lee SG, "Spread of CTX-M-type extended-spectrum b-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital", Diagn Microbiol Infect Dis, 63, pp.76~80 (2009)
  16. 고지선, "대전지역에서 분리된 Escherichia coli 와 Klebsiella pneumoniae 의Extended-Spectrum -lactamase 생성 현황", The Korean Journal of Laboratory Medicine, 27(5), pp.344~350 (2007), 대한진단검사의학회
  17. 강지혜, "Ambler Class A Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의국내분리현황", 대한임상미생물학회지, 8(1), pp.17~25 (2005), 대한임상미생물학회
  18. Bae IK, "High prevalence of SHV-12 and the emergence of CTX-M-12 in clinical isolates of Klebsiella pneumoniae from Korea", Int J Antimicrob Agents, 29, pp.362~364 (2007)
  19. 배일권, "CTX-M-12와 새로운 CTX-M형 Extended-Spectrum β-Lactamase생성 Klebsiella pneumoniae의 출현", The Korean Journal of Laboratory Medicine, 26(1), pp.21~26 (2006), 대한진단검사의학회
  20. Thomson KS, "Comparison of Phoenix and VITEK 2 extended-spectrum-blactamase detection tests for analysis of Escherichia coli and Klebsiella isolates with well-characterized b-lactamases", J Clin Microbiol, 45, pp.2380~2384 (2007)

관련 논문AI추천

Molecular Characteristics of Extended Spectrum β-Lactamases in Escherichia coli and Klebsiella pneumoniae and the Prevalence of qnr in Extended Spectrum β-Lactamase Isolates in a Tertiary Care Hospital in Korea김명희, 박경선, 서진태, 이희주연세대학교의과대학 · Yonsei Medical Journal2010 · 제51권 제5호Ambler Class A Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의국내분리현황어영, 강정옥, 강지혜, 권수봉, 김의종, 박연준, 배일권, 신종희, 안지영, 용동은, 우건조, 이경원, 이위교, 이종욱, 정석훈, 홍성근대한임상미생물학회 · Annals of Clinical Microbiology2005 · 제8권 제1호Prevalence of the Extended-Spectrum β-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli어영, 강현경, 김재석, 김주원, 박용정, 배일권, 이경원, 정석훈대한진단검사의학회 · Annals of Laboratory Medicine2009 · 제29권 제3호대전지역에서 분리된 Escherichia coli 와 Klebsiella pneumoniae 의Extended-Spectrum -lactamase 생성 현황고지선, 구선회, 권계철, 박종우, 성지연, 신소연대한진단검사의학회 · Annals of Laboratory Medicine2007 · 제27권 제5호CTX-M-12와 새로운 CTX-M형 Extended-Spectrum β-Lactamase생성 Klebsiella pneumoniae의 출현어영, 강정옥, 곽효선, 김의종, 박연준, 배일권, 신종희, 안지영, 용동은, 우건조, 이경원, 이성희, 이위교, 이종욱, 정석훈, 홍성근대한진단검사의학회 · Annals of Laboratory Medicine2006 · 제26권 제1호An Increase in the Clinical Isolation of Acquired AmpC β-Lactamase-Producing Klebsiella pneumoniae in Korea from 2007 to 2010김재석, 김택경, 김한성, 박민정, 송원근, 이재갑대한진단검사의학회 · Annals of Laboratory Medicine2013 · 제33권 제5호

이 간행물의 다른 논문 (28건)

A Study of Mixed Phenotype Acute Leukemia Based on the 2008 World Health Organization Classification박준홍, 한경자, 이재욱, 임지향, 김용구, 김학기, 김명신, 이석, 정낙균, 조빈, 채효진pp. 525-532Minimal Residual Disease Detection in Acute Leukemia Patients by Flow Cytometric Assay of Cross-lineage Antigen Expression조영욱, 이제환, 김미정, 이정희, 서종진, 차충환, 지현숙, 박찬정, 임호준, 이규형, 장성수pp. 533-539Differential Blast Counts Obtained by Automated Blood Cell Analyzers임지향, 오은지, 박연준, 김용구, 채효진, 정승원, 한경자pp. 540-546Prognostic Impact of Helicobacter pylori Infection and Eradication Therapy in Gastric Mucosa-associated Lymphoid Tissue Lymphoma허주령, 박상혁, 지현숙, 박찬정, 박서진, 장성수pp. 547-553Significance of Epstein-Barr Virus DNA Quantitation in Donors of Hematopoietic Stem Cell Transplantation조병식, 이종욱, 한경자, 임지향, 김용구, 김명신, 채효진, 민우성, 정승원pp. 554-558
원문 보기