Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-β-lactamase nonproducers.
Abstract
In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-β-lactamase nonproducers.
참고문헌 (14)
- Patel G, "Status report on carbapenemases: challenges and prospects", Expert Rev Anti Infect Ther, 9, pp.555~570 (2011)
- Gupta N, "Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention", Clin Infect Dis, 53, pp.60~67 (2011)
- Miriagou V, "Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues", Clin Microbiol Infect, 16, pp.112~122 (2010)
- Cornaglia G, "The emerging threat of acquired carbapenemases in Gram-negative bacteria", Clin Microbiol Infect, 16, pp.99~101 (2010)
- Ambretti S, "A carbapenemresistant Klebsiella pneumoniae isolate harboring KPC-1 from Italy", New Microbiol, 33, pp.281~282 (2010)
- Villegas MV, "First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem-hydrolyzing β-lactamase", Antimicrob Agents Chemother, 51, pp.1553~1555 (2007)
- Wei ZQ, "Plasmid-mediated KPC-2 in a Klebsiella pneumoniae isolate from China", Antimicrob Agents Chemother, 51, pp.763~765 (2007)
- Yigit H, "Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae", Antimicrob Agents Chemother, 45, pp.1151~1161 (2001)
- 박영경, "KPC-Producing Extreme Drug-Resistant Klebsiella pneumoniae Isolate from a Patient with Diabetes Mellitus and Chronic Renal Failure on Hemodialysis in South Korea", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 54, pp.2278~2279, AMER SOC MICROBIOLOGY
- 노경호, "Isolation of a Klebsiella pneumoniae Isolate of Sequence Type 258 Producing KPC-2 Carbapenemase in Korea", Annals of Laboratory Medicine, 31(4), pp.298~301 (2011), 대한진단검사의학회
- Thomson KS, "Extended-Spectrum-β-Lactamase, AmpC, and Carbapenemase issues", J Clin Microbiol, 48, pp.1019~1025 (2010)
- Pasteran F, "Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae", J Clin Microbiol, 47, pp.1631~1639 (2009)
- Girlich D, "Novel ambler class A carbapenem-hydrolyzing β-lactamase from a Pseudomonas fluorescens isolate from the Seine River, Paris, France", Antimicrob Agents Chemother, 54, pp.328~332 (2010)
- Henriques I, "Molecular characterization of a carbapenem-hydrolyzing class A β-lactamase, SFC-1, from Serratia fonticola UTAD54", Antimicrob Agents Chemother, 48, pp.2321~2324 (2004)