PON1과 ALDH2 유전자 다형성에 따른요중 페놀농도와 요중 TBARS농도 사이의 관련성
Relationship between Urinary Phenol and TBARS Concentrations According to the ALDH2 and PON1 Genotypes
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초록
연구배경 직업적 벤젠 노출이 없는 일반인에서 요중 페놀 농도와 지질과산화 정도 사이의 관련성을 평가하며, 이러한 관련성이 ALDH2와 PON1 유전자 다형성에 따라 달라지는가를 평가하기 위하여 본 연구를 시행하였다. 방 법 직업적 벤젠 노출력이 없는 155명에 대하여 흡연 여부를 확인하고, 요중 페놀 농도와 요중 thiobarbituric acid reactive substances(이하 TBARS)의 농도를 측정하였으며, ALDH2 및 PON1 유전자 다형성을 조사하였다. 요중 페놀과 TBARS 농도의 자연대수값 사이의 관련성을 상관분석으로 평가하고, 이를 ALDH2와 PON1 유전자형에 따라 반복하였다. 결 과 대상자의 요중 페놀 농도와 요중 TBARS 농도는 여자가 남자보다 높았으나, 흡연 유무, ALDH2 유전자형, PON1 유전자형에 따라서는 유의한 차이를 보이지 않았다. 요중 페놀 농도와 요중 TBARS 농도의 자연대수값 사이에는 유의한 관련성이 있었다. 남자, 비흡연자, ALDH2 *1/*1 유전자형, PON1 Q/R 유전자형, 그리고 특히 ALDH2 *1/*1형이며 PON1 Q/Q 혹은 Q/ R 유전자형인 군에서 두 변수간의 상관관계가 유의하였다. 결 론 요중 페놀 농도가 증가함에 따라 지질과산화가 더 심해지며, 이러한 효과는 ALDH2 *1/*1유전자형과 PON1 유전자 Q 대립유전자를 가지고 있는 사람에서 더 두드러지게 나타난다.
Abstract
Background This study was performed to test the relationship between the levels of phenol and thiobarbituric acid reactive substance (TBARS) in urines from individuals who were not occupationally exposed to benzene, and to evaluate the effects of ALDH2 gene and PON1 gene polymorphism on the relationship. Methods One hundred and fifty-five persons (89 males and 66 females) who visited a university hospital for a screening test and were not occupationally exposed to benzene were the subjects of this study. Information about smoking status was collected through interviews. Urinary phenol and TBARS concentrations were measured, and genotypes of ALDH2 and PON1 genes were identified. Urinary phenol and TBARS levels were compared according to sex, smoking status and the ALDH2 and PON1 genotypes. The correlation between ln(phenol) and ln(TBARS) was statistically tested in all subjects and in subgroups with different ALDH2 and PON1 genotypes. Results Urinary phenol and TBARS concentrations were higher in females than in males, but no statistical difference was found in the comparisons according to sex, smoking status, and the ALDH2 and PON1 genotypes. There was a signigicant correlation between ln(phenol) and ln(TBARS), and especially in males, non-smokers or individuals with ALDH2 *1/*1 or PON1 Q/R genotype. Those with ALDH2 *1/*1 genotype and PON1 Q/Q or Q/R genotype showed a higher and significant correlation between between the natural logarithmic values of urinary phenol and TBARS concentrations. Conclusions An increase in urinary phenol concentration leads to a more severe lipid peroxidation or oxidative stress. Individuals with ALDH2 *1/*1 genotype and PON1 Q/Q or Q/R genotype would be more sensitive to the phenol-related lipid peroxidation.
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