Lipid and Citric Acid Production by Wild Yeasts Grown in Glycerol
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In this study, crude glycerol was used as a carbon source in the cultivation of wild yeasts,aiming at the production of microbial lipids and citric acid. Forty yeasts of different sourceswere tested concerning their growth in crude and commercial glycerol. Four yeasts (Lindnerasaturnus UFLA CES-Y677, Yarrowia lipolytica UFLA CM-Y9.4, Rhodotorula glutinis NCYC 2439,and Cryptococcus curvatus NCYC 476) were then selected owing to their ability to grow in pure(OD600 2.133, 1.633, 2.055, and 2.049, respectively) and crude (OD600 2.354, 1.753, 2.316, and2.281, respectively) glycerol (10%, 20%, and 30%). Y. lipolytica UFLA CM-Y9.4 was selected forits ability to maintain cell viability in concentrations of 30% of crude glycerol, and highglycerol intake (18.907 g/l). This yeast was submitted to lipid production in 30 g/l of crudeglycerol, and therefore obtained 63.4% of microbial lipids. In the fatty acid profile, there was apredominance of stearic (C18:0) and palmitic (C16:0) acids in the concentrations of 87.64% and74.67%, respectively. We also performed optimization of the parameters for the production ofcitric acid, which yielded a production of 0.19 g/l of citric acid in optimum conditions (38.4 g/lof crude glycerol, agitation of 184 rpm, and temperature of 30oC). Yarrowia lipolytica UFLACM-Y9.4 presented good lipid production when in the concentration of 30 g/l of glycerol. These data may be used for production in large quantities for the application of industrialbiodiesel.
Abstract
In this study, crude glycerol was used as a carbon source in the cultivation of wild yeasts,aiming at the production of microbial lipids and citric acid. Forty yeasts of different sourceswere tested concerning their growth in crude and commercial glycerol. Four yeasts (Lindnerasaturnus UFLA CES-Y677, Yarrowia lipolytica UFLA CM-Y9.4, Rhodotorula glutinis NCYC 2439,and Cryptococcus curvatus NCYC 476) were then selected owing to their ability to grow in pure(OD600 2.133, 1.633, 2.055, and 2.049, respectively) and crude (OD600 2.354, 1.753, 2.316, and2.281, respectively) glycerol (10%, 20%, and 30%). Y. lipolytica UFLA CM-Y9.4 was selected forits ability to maintain cell viability in concentrations of 30% of crude glycerol, and highglycerol intake (18.907 g/l). This yeast was submitted to lipid production in 30 g/l of crudeglycerol, and therefore obtained 63.4% of microbial lipids. In the fatty acid profile, there was apredominance of stearic (C18:0) and palmitic (C16:0) acids in the concentrations of 87.64% and74.67%, respectively. We also performed optimization of the parameters for the production ofcitric acid, which yielded a production of 0.19 g/l of citric acid in optimum conditions (38.4 g/lof crude glycerol, agitation of 184 rpm, and temperature of 30oC). Yarrowia lipolytica UFLACM-Y9.4 presented good lipid production when in the concentration of 30 g/l of glycerol. These data may be used for production in large quantities for the application of industrialbiodiesel.
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