A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology
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Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from marine environments could have interesting characteristics for industrial applications. However, the application of marine yeast in research or industry is currently very limited owing to the lack of a suitable isolation method. Current methods for isolation suffer from fungal interference and/or low number of yeast isolates. In this paper, an efficient and nonlaborious isolation method has been developed and successfully isolated large numbers of yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA. These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose, mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC 2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved sugar utilization using seawater-based media was confirmed and, therefore, they could potentially be utilized in fermentations using marine biomass in seawater media, particularly for the production of bioethanol and other biochemical products.
Abstract
Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from marine environments could have interesting characteristics for industrial applications. However, the application of marine yeast in research or industry is currently very limited owing to the lack of a suitable isolation method. Current methods for isolation suffer from fungal interference and/or low number of yeast isolates. In this paper, an efficient and nonlaborious isolation method has been developed and successfully isolated large numbers of yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA. These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose, mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC 2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved sugar utilization using seawater-based media was confirmed and, therefore, they could potentially be utilized in fermentations using marine biomass in seawater media, particularly for the production of bioethanol and other biochemical products.
참고문헌 (47)
- Ahearn DG, "Ecology and characterization of yeasts from aquatic regions of South Florida", Mar. Biol, 1, pp.291~308 (1968)
- Bieleski RL, "Plant Carbohydrates I", pp.158~192 (1982), Springer
- Burgaud G, "Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna", FEMS Microbiol. Ecol, 73, pp.121~133 (2010)
- Cadete R, "Biofuels in Brazil", pp.255~279 (2014), Springer International Publishing
- Cavka A, "Comparison of the growth of filamentous fungi and yeasts in lignocellulose-derived media", Biocatal. Agric. Biotechnol, 3, pp.197~204 (2014)
- Chi Z, "Production, characterization and gene cloning of the extracellular enzymes from the marine-derived yeasts and their potential applications", Biotechnol. Adv, 27, pp.236~255 (2009)
- Dinesh Kumar S, "Biosynthesis of silver nanoparticles from marine yeast and their antimicrobial activity against multidrug resistant pathogens", Pharmacol. Online, 3, pp.1100~1111 (2011)
- Fell JW, "Collection and identification of marine yeasts, Methods in Microbiology", pp.347~356 (2001), Academic Press
- Fell J, "Five new species of yeasts from fresh water and marine habitats in the Florida Everglades", Antonie Van Leeuwenhoek, 99, pp.533~549 (2011)
- Foschino R, "Comparison of cultural methods for the identification and molecular investigation of yeasts from sourdoughs for Italian sweet baked products", FEMS Yeast Res, 4, pp.609~618 (2004)
- Greetham D, "Development of a phenotypic assay for characterisation of ethanologenic yeast strain sensitivity to inhibitors released from lignocellulosic feedstocks", J. Ind. Microbiol. Biotechnol, 41, pp.931~945 (2014)
- Guo F-J, "A novel killer toxin produced by the marine-derived yeast Wickerhamomyces anomalus YF07b", Antonie Van Leeuwenhoek, 103, pp.737~746 (2013)
- Jones EBG, "Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota", Fungal Diver, 73, pp.1~72 (2015)
- Karsten U, "Characterization of mannitol metabolism in the mangrove red alga Caloglossa leprieurii (Montagne)", J.Agardh. Planta, 201, pp.173~178 (1997)
- Khambhaty Y, "Bioethanol from macroalgal biomass:utilization of marine yeast for production of the same", Bioenergy Res, 6, pp.188~195 (2013)
- Kohlmeyer J, "Yeasts", pp.556~606 (1979), Marine Mycology Academic Press
- Koop K, "Mannitol-fermenting bacteria as evidence for export from kelp beds", Limnol. Oceanogr, 27, pp.950~954 (1982)
- Kumar S, "Bioethanol production from Gracilaria verrucosa, a red alga, in a biorefinery approach", Bioresour. Technol, 135, pp.150~156 (2013)
- Kurtzman CP, "Biodiversity and Ecophysiology of Yeasts", pp.11~30 (2006), Springer
- Kurtzman C, "Comparative Genomics", pp.29~46 (2006), Springer
- Kurtzman CP, "Advances in yeast systematics and phylogeny and their use as predictors of biotechnologically important metabolic pathways", FEMS Yeast Res, 15, pp.fov050~ (2015)
- Kutty SN, "Marine yeasts from the slope sediments of Arabian Sea and Bay of Bengal", 박사, pp.2009
- Kutty SN, "Marine yeasts - a review", Yeast, 25, pp.465~483 (2008)
- Lin CSK, "A seawater-based biorefining strategy for fermentative production and chemical transformations of succinic acid", Energy Environ. Sci, 4, pp.1471~1479 (2011)
- Mitchell TG, "Comparison of 5.8S ribosomal DNA sequences among the basidiomycetous yeast genera Cystofilobasidium, Filobasidium and Filobasidiella", J. Med. Vet. Mycol, 30, pp.207~218 (1992)
- Nagahama T, "Kluyveromyces nonfermentans sp. nov., a new yeast species isolated from the deep sea", Int. J. Syst. Bacteriol, 49, pp.1899~1905 (1999)
- Nasr NF, "Microbiological quality of active dry and compressed baker’s yeast sold in Egypt", J. Pure Appl. Microbiol, 4, pp.455~462 (2010)
- Obara N, "Development of a simple isolation method for yeast Saccharomyces cerevisiae with high fermentative activities from coastal waters", Stud. Sci. Technol, 4, pp.71~76 (2015)
- Oshoma CE, "Screening of non-Saccharomyces cerevisiae strains for tolerance to formic acid in bioethanol fermentation", PLoS One, 10, pp.e0135626~ (2015)
- Ostergaard S, "Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network", Nat. Biotechnol, 18, pp.1283~1286 (2000)
- Pincus DH, "Yeast identification - past, present, and future methods", Med. Mycol, 45, pp.97~121 (2007)
- Praphailong W, "Evaluation of the Biolog system for the identification of food and beverage yeasts", Lett. Appl. Microbiol, 24, pp.455~459 (1997)
- Reed RH, "The osmotic role of mannitol in the Phaeophyta: an appraisal", Phycologia, 24, pp.35~47 (1985)
- Rhishipal R, "Selection of marine yeasts f or the generation of single cell protein from prawn-shell waste", Bioresour. Technol, 65, pp.255~256 (1998)
- Sarkar S, "Bioprocessing data for the production of marine enzymes", Mar. Drugs, 8, pp.1323~1372 (2010)
- Seshadri R, "Seaweeds as a reservoir of Candida yeasts in inshore waters", Mar. Biol, 30, pp.105~117 (1975)
- Silvi S, "Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide", Microb. Cell Fact, 12, pp.10~ (2013)
- Urano N, "Distribution of halotolerant and", or fermentative yeasts in aquatic environments, 2001, pp.87, 7~
- Wang L, "Isolation and characterization of Candida membranifaciens subsp. flavinogenie W14-3, a novel riboflavin-producing marine yeast", Microbiol. Res, 163, pp.255~266 (2008)
- Wenger JW, "Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae", PLoS Genet, 6, pp.e1000942~ (2010)
- White T, "PCR Protocols: A Guide to Methods and Applications", pp.315~322 (1990), Academic Press
- White WL, "Utilisation of mannitol by temperate marine herbivorous fishes", J. Exp. Mar. Biol. Ecol, 391, pp.50~56 (2010)
- Wickerham LJ, "Taxonomy of yeasts", US Dept. Agric. Tech. Bull, 1029, pp.1~56 (1951)
- Wimalasena TT, "Phenotypic characterisation of Saccharomyces spp. yeast for tolerance to stresses encountered during fermentation of lignocellulosic residues to produce bioethanol", Microb. Cell Fact, 13, pp.47~ (2014)
- Zaki AM, "Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol", J. Ind. Microbiol. Biotechnol, 41, pp.1627~1636 (2014)
- Zaky AS, "The isolation of novel marine yeasts;a new procedure", New Biotechnol, 31(Suppl. 132), pp.1939~ (2014)[DOI]
- Zaky AS, "Marine yeast isolation and industrial application", FEMS Yeast Res, 14, pp.813~825 (2014)