Rhodobacter capsulatus hemA 유전자 발현 대장균에 의한 5-Aminolevulinic Acid 생산의 최적화
Optimizing the Production of 5-Aminolevulinic Acid by Recombinant Escherichia coli Containing the Rhodobacter capsulatus hemA Gene
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초록
Recombinant Escherichia coli BLR(DE3) harboring the hemA gene from Rhodobacter capsulatus under the control of a constitutive promoter, which we constructed previously, was used for the extracellular production of 5-aminolevulinic acid (ALA). The effects of several factors on ALA production were investigated in flask culture. ALA production by the recombinant E. coli was more efficient at 30℃ than 37℃. The glycine concentration had an important effect on cell growth. Glycine and succinic acid concentration of 5-10 and 10-20 g/L, respectively, resulted in high ALA production. In addition, the partial replacement of succinic acid by sodium glutamate increased the ALA production. The ALA production was inhibited by the presence of glucose in the medium. Using the optimal conditions, an ALA concentration of 8.2 g/L was achieved in jar fermentation without an added inducer or ALA dehydratase inhibitor; this is the highest reported concentration.
Abstract
Recombinant Escherichia coli BLR(DE3) harboring the hemA gene from Rhodobacter capsulatus under the control of a constitutive promoter, which we constructed previously, was used for the extracellular production of 5-aminolevulinic acid (ALA). The effects of several factors on ALA production were investigated in flask culture. ALA production by the recombinant E. coli was more efficient at 30℃ than 37℃. The glycine concentration had an important effect on cell growth. Glycine and succinic acid concentration of 5-10 and 10-20 g/L, respectively, resulted in high ALA production. In addition, the partial replacement of succinic acid by sodium glutamate increased the ALA production. The ALA production was inhibited by the presence of glucose in the medium. Using the optimal conditions, an ALA concentration of 8.2 g/L was achieved in jar fermentation without an added inducer or ALA dehydratase inhibitor; this is the highest reported concentration.
참고문헌 (32)
- Avissar, Y. J, "Identification of the enzymatic basis for ä-aminolevulinic acid auxotrophy in a hemA mutant of Escherichia coli", J. Bacteriol, 171, pp.2919~2924 (1989)
- Beale, S. J, "The Biosynthesis of δ-aminolevulinic acid in higher plants. II. Formation of 14C-δ-aminolevulinic acid from labeled precursors in greening plant tissue", Plant Physiol, 53, pp.297~303 (1974)
- Berg, K, "Porphyrin-related photosensitizers for cancer imaging and therapeutic applications", J. Microsc., 218, pp.133~147 (2005)
- Bykhovsky, V. Y, "The crucial contribution of starved resting cells to the elucidation of the pathway of vitamin B12 biosynthesis", Critical Rev. Biotechnol, 17(1), pp.21~37 (1997)
- Y.J. Chen, "Evaluation of δ-Aminolevulinic Acid on Serum Iron Status, Blood Characteristics, Egg Performance and Quality in Laying Hens", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21(9), pp.1355~1360 (2008), 아세아·태평양축산학회
- Choi, C, "Optimization of extracellular 5-aminolevulinic acid production from Escherichia coli transformed with ALA synthase gene of Bradyrhizobium japonicum", Biotechnol. Lett, 21, pp.551~554 (1999)
- Choi, Han-Pil, "Extracellular 5-Aminolevulinic Acid Production by Escherichia coli Containingthe Rhodopseudomonas palustris KUGB306 hemA Gene", Journal of Microbiology and Biotechnology, 18(6), pp.1136~1140 (2008), 한국미생물·생명공학회
- 이종일, "유전자 재조합 대장균에 의한 5-Aminolevulinic Acid (ALA)의 생산 및 공정 모니터링 I. ALA의 생산 특성", KSBB Journal, 19(1), pp.17~26 (2004), 한국생물공학회
- Dempsey, W. B, "Lysis of Escherichia coli by glycine is potentiated by pyridoxine starvation", J. Bacteriol, 116(1), pp.373~377 (1973)
- Ferreira, G, "5-Aminolevulinate synthase and the first step of heme biosynthesis", J. Bioenerg. Biomembr., 27, pp.151~159 (1995)
- Fu, W, "Enhancement of 5-aminolevulinate production with recombinant Escherichia coli using batch and fed-batch culture system", Bioresource Technol, 99, pp.4864~4870 (2008)
- Halpern, Y. S, "Effect of glucose on the utilization of succinate and the activity of tricarboxylic acid-cycle enzymes in Escherichia coli", Biochim Biophys Acta., 93, pp.228~236 (1964)
- Hammets, W, "Mode of action of glycine on the biosynthesis of peptidoglycan", J. Bacteriol, 116(2), pp.1029~1053 (1973)
- Han, L, "Effect of glycine on the cell yield and growth rate of Escherichia coli: evidence for cell-density-dependent glycine degradation as determined by (13)C NMR spectroscopy", J. Biotechnol, 92(3), pp.237~249 (2002)
- KANG, DAE-KYUNG, "Cloning and Expression of the Rhodobacter capsulatus hemA Gene in E. colifor the Production of 5-Aminolevulinic Acid", Journal of Microbiology and Biotechnology, 14(6), pp.1327~1332 (2004), 한국미생물·생명공학회
- Kikuchi, G, "The enzymatic synthesis of ä-aminolevulinic acid", J. Biol. Chem, 233, pp.1214~1219 (1958)
- Lee, D.-H, "Inhibition of 5-aminolevulinic acid dehydratase in recombinant Escherichia coli using Dglucose", Enz. Microbial Technol, 32, pp.27~34 (2003)
- Lee, D.-H, "Process strategies to enhance the production of 5-aminolevulinic acid with recombinant", E. coli. J. Microbiol. Biothchnol, 14(6), pp.1310~1317 (2004)
- Lee, D.-H, "Effect of culture conditions on production of 5- aminolevulinic acid by recombinant Escherichia coli", Biosci. Biotechnol. Biochem, 69(3), pp.470~476 (2005)
- Lo, T. C. Y, "Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells", J. Biol. Chem, 247(19), pp.6323~6331 (1972)
- R. D. Mateo, "Use of δ-Aminolevulinic Acid in Swine Diet: Effect on Growth Performance, Behavioral Characteristics and Hematological", Immune Status in Nursery Pigs, 2006, pp.19(1), 97~101[DOI]
- Mauzerall, D, "The occurance and determination of δ-aminolevulinic acid and porphobilinogen in urine", J. Biol. Chem, 219(11), pp.435~446 (1956)
- Miyachi, N, "Preparation and chemical properties of 5-aminolevulinic acid and its derivatives", Porphyrins, 7, pp.342~347 (1998)
- Nam, T.-W, "Glucose repression of the Escherichia coli sdhCDAB operon, revisited: regulation by the CRP·cAMP complex", Nucleic Acids Res, 33(21), pp.6712~6722 (2005)
- Poo, H, "Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-aaa", Biotechnol. Lett, 24, pp.1185~1189 (2002)
- Qin, G, "Effect of medium composition on production of 5-aminolevulinic acid by recombinant Escherichia coli", J. Biosci. Bioeng, 102(4), pp.316~322 (2006)
- Rebeiz, C. A, "Photodynamic herbicides. I. Concepts and phenomenology", Enzyme Microb. Technol, 6, pp.390~401 (1984)
- Sasikala, Ch, "5-Aminolevulinic acid: a potential herbicide", insecticide from microorganisms, 1994, pp.10, 451~459
- Sasaki, K, "Biosynthesis, biotechnological production and applications of 5-aminolevulinic acid", . Microbiol. Biotechnol., 58, pp.23~29 (2002)
- SHIN, JEONG-AH, "5-Aminolevulinic Acid Biosynthesis in Escherichia coli CoexpressingNADP-dependent Malic Enzyme and 5-Aminolevulinate Synthase", Journal of Microbiology and Biotechnology, 17(9), pp.1579~1584 (2007), 한국미생물·생명공학회
- Takahashi, Y, "Effect of glucose and cyclic Adenosine 3', 5'-monophosphate on the synthesis of succinate dehydrogenase and isocitrate lyase in Escherichia coli", J. Biochem, 78(5), pp.1097~1100 (1975)
- Van der Werf, M. J, "1996. 5-Aminolevulinate production by Escherichia coli containing the Rhodobacter sphaeroides hemA gene", Appl. Environ. Microbiol, 62, pp.3560~3566 (1996)