Isolation and characterization of a lytic Salmonella Typhimurium-specific phage as a potential biofilm control agent
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study aimed to characterize a lytic Salmonella Typhimurium-specific (ST) phage and its biofilm control capability against S. Typhimurium biofilm on polypropylene surface. ST phage was isolated, propagated, and purified from water used in a slaughterhouse. The morphology of ST phage was observed via transmission electron microscopy. Its bactericidal effect was evaluated by determining bacterial concentrations after the phage treatment at various multiplicities of infection (MOIs) of 0.01, 1.0, and 100. Once the biofilm was formed on the polypropylene tube after incubation at 37℃ for 48 h, the phage was treated and its antibiofilm capability was determined using crystal violet staining and plate count method. The phage was isolated and purified at a final concentration of ∼11 log PFU/mL. It was identified as a myophage with an icosahedral head (∼104 nm) and contractile tail (∼90-115 nm). ST phage could significantly decrease S. Typhimurium population by ∼2.8 log CFU/mL at an MOI of 100. After incubation for 48 h, biofilm formation on polypropylene surface was confirmed with a bacterial population of ∼6.9 log CFU/cm2. After 1 h treatment with ST phage, the bacterial population in the biofilm was reduced by 2.8 log CFU/cm2. Therefore, these results suggest that lytic ST phage as a promising biofilm control agent for eradicating S. Typhimurium biofilm formed on food contact surfaces.
Abstract
This study aimed to characterize a lytic Salmonella Typhimurium-specific (ST) phage and its biofilm control capability against S. Typhimurium biofilm on polypropylene surface. ST phage was isolated, propagated, and purified from water used in a slaughterhouse. The morphology of ST phage was observed via transmission electron microscopy. Its bactericidal effect was evaluated by determining bacterial concentrations after the phage treatment at various multiplicities of infection (MOIs) of 0.01, 1.0, and 100. Once the biofilm was formed on the polypropylene tube after incubation at 37℃ for 48 h, the phage was treated and its antibiofilm capability was determined using crystal violet staining and plate count method. The phage was isolated and purified at a final concentration of ∼11 log PFU/mL. It was identified as a myophage with an icosahedral head (∼104 nm) and contractile tail (∼90-115 nm). ST phage could significantly decrease S. Typhimurium population by ∼2.8 log CFU/mL at an MOI of 100. After incubation for 48 h, biofilm formation on polypropylene surface was confirmed with a bacterial population of ∼6.9 log CFU/cm2. After 1 h treatment with ST phage, the bacterial population in the biofilm was reduced by 2.8 log CFU/cm2. Therefore, these results suggest that lytic ST phage as a promising biofilm control agent for eradicating S. Typhimurium biofilm formed on food contact surfaces.
참고문헌 (42)
- Azeredo J, "Critical review on biofilm methods", Crit Rev Microbiol, 43, pp.313~351 (2017)
- Azeredo J, "Targeting biofilms using phages and their enzymes", Curr Opin Biotechnol, 68, pp.251~261 (2021)
- "waterborne, and environmental diseases"[URL]
- Chang C, "Bacteriophage-mediated control of biofilm : A promising new dawn for the future", Front Microbiol, 13, pp.825828~ (2022)
- 최인영, "Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection", Journal of Microbiology and Biotechnology, 27(12), pp.2151~2155 (2017), 한국미생물·생명공학회
- Corcoran M, "Commonly used disinfectants fail to eradicate Salmonella enterica biofilms from food contact surface materials", Appl Environ Microbiol, 80, pp.1507~1514 (2014)
- Duc HM, "Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157 : H7 in different food matrices", Food Res Int, 131, pp.108977~ (2020)
- Endersen L, "Phage therapy in the food industry", Annu Rev Food Sci Technol, 5, pp.327~349 (2014)
- Fu W, "Bacteriophage cocktail for the prevention of biofilm formation by Pseudomonas aeruginosa on catheters in an in vitro model system", Antimicrob Agents Chemother, 54, pp.397~404 (2010)
- Garcia KCDOD, "Bacteriophage use to control Salmonella biofilm on surfaces present in chicken slaughterhouses", Poult Sci, 96, pp.3392~3398 (2017)
- 곽경민, "Isolation and Characterization of a Lytic and Highly Specific Phage against Yersinia enterocolitica as a Novel Biocontrol Agent", Journal of Microbiology and Biotechnology, 28(11), pp.1946~1954 (2018), 한국미생물·생명공학회
- Iibuchi R, "Survival of Salmonella on a polypropylene surface under dry conditions in relation to biofilm-formation capability", J Food Prot, 73, pp.1506~1510 (2010)
- Islam MS, "Application of a phage cocktail for control of Salmonella in foods and reducing biofilms", Viruses, 11, pp.841~ (2019)
- Islam MS, "Application of a broad range lytic phage LPST94 for biological control of Salmonella in foods", Microorganisms, 8, pp.247~ (2020)
- Kalatzis PG, "Isolation and characterization of two lytic bacteriophages, φst2 and φgrn1; phage therapy application for biological control of Vibrio alginolyticus in aquaculture live feeds", PloS One, 11, pp.e0151101~ (2016)
- Kim SH, "Characterization of a new and efficient polyvalent phage infecting E. coli O157 : H7, Salmonella spp., and Shigella sonnei", Microorganisms, 9, pp.2105~ (2021)
- 김수현, "Characterization of a lytic phage KFS-EC3 infecting multiple foodborne pathogens", Food Science and Preservation, 29(7), pp.1022~1034 (2022), 한국식품저장유통학회
- Kuzminska-Bajor M, "Bacteriophage cocktail can effectively control Salmonella biofilm in poultry housing", Front Microbiol, 13, pp.901770~ (2022)
- Kwon J, "Isolation and characterization of Salmonella jumbo-phage pSal-SNUABM-04", Viruses, 13, pp.27~ (2021)
- Lang LH, "FDA approves use of bacteriophages to be added to meat and poultry products", Gastroenterology, 131, pp.1370~ (2006)
- Lee C, "Isolation and characterization of a novel Escherichia coli O157 : H7-specific phage as a biocontrol agent", J Environ Health Sci Engineer, 18, pp.189~199 (2020)
- Liu A, "Characterization of the narrow-spectrum bacteriophage LSE7621 towards Salmonella Enteritidis and its biocontrol potential on lettuce and tofu", LWT, 118, pp.108791~ (2020)
- Meng X, "Application of a bacteriophage lysin to disrupt biofilms formed by the animal pathogen Streptococcus suis", Appl Environ Microbiol, 77, pp.8272~8279 (2011)
- Milho C, "Control of Salmonella Enteritidis on food contact surfaces with bacteriophage PVP-SE2", Biofouling, 34, pp.753~768 (2018)
- Mohamed A, "Bacteriophage ZCSE2 is a potent antimicrobial against Salmonella enterica serovars : Ultrastructure, genomics and efficacy", Viruses, 12, pp.424~ (2020)
- 오준현, "Recent Trends in Salmonella Outbreaks and Emerging Technology for Biocontrol of Salmonella Using Phages in Foods: A Review", Journal of Microbiology and Biotechnology, 27(12), pp.2075~2088 (2017), 한국미생물·생명공학회
- Oliveira J, "Bacteriophage therapy as a bacterial control strategy in aquaculture", Aquaculture Int, 20, pp.879~910 (2012)
- Park M, "Characterization and comparative genomic analysis of a novel bacteriophage, sfp10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157 : H7", Appl Environ Microbiol, 78, pp.58~69 (2012)
- Park MK, "Evaluation of phage-based magnetoelastic biosensors for direct detection of Salmonella Typhimurium on spinach leaves", Sens Actuators B Chem, 176, pp.1134~1140 (2013)
- Reij M, "Recontamination as a source of pathogens in processed foods", Int J Food Microbiol, 91, pp.1~11 (2004)
- Rodrigues D, "Salmonella enterica Enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials", J Food Prot, 74, pp.32~37 (2011)
- Sadekuzzaman M, "Application of bacteriophages for the inactivation of Salmonella spp. in biofilms", Food Sci Technol Int, 24, pp.424~433 (2018)
- Sarhan WA, "Phage approved in food, why not as a therapeutic?", Expert Rev Anti Infect Ther, 13, pp.91~101 (2015)
- Scallan E, "Foodborne illness acquired in the United States-Major pathogens", Emerg Infect Dis, 17, pp.7~ (2011)
- Singh A, "Rice seed priming with picomolar rutin enhances rhizospheric Bacillus subtilis CIM colonization and plant growth", PloS One, 11, pp.e0146013~ (2016)
- Suslow T, "Potential for Transference of Inoculated and Indigenous Bacteria from the Non-Wounded Rind of Melons to the Interior Edible Flesh", 2000Annual Meeting, International Association of Food Protection (2000)
- Birendra R. Tiwari, "A Virulent Salmonella enterica Serovar Enteritidis Phage SE2 with a Strong Bacteriolytic Activity of Planktonic and Biofilmed Cells", Journal of Bacteriology and Virology, 43(3), pp.186~194 (2013), 대한미생물학회[DOI]
- Vert M, "Terminology for biorelated polymers and applications(iupac recommendations 2012)", Pure Appl Chem, 84, pp.377~410 (2012)
- Wang C, "Characterization of a broad host-spectrum virulent Salmonella bacteriophage fmb-p1 and its application on duck meat", Virus Res, 236, pp.14~23 (2017)
- Wu X, "Photo aging and fragmentation of polypropylene food packaging materials in artificial seawater", Water Research, 188, pp.116456~ (2021)
- Zhao X, "Biofilm formation and control strategies of foodborne pathogens : Food safety perspectives", RSC Adv, 7, pp.36670~36683 (2017)
- Zinke M, "Major tail proteins of bacteriophages of the order Caudovirales", J Biol Chem, 298, pp.101472~ (2022)