Inhibitory Effects of Flavonoids from Spatholobus suberectus on Sortase A and Sortase A-Mediated Aggregation of Streptococcus mutans
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Seven flavonoids were isolated from Spatholobus suberectus via repetitive column chromatography and high-performance liquid chromatography. The chemical structures of these compounds were identified by spectroscopic analysis and comparison with values reported in the literature. Among the flavonoids tested, 7-hydroxy-6-methoxyflavanone (1) and formononetin (4) exhibited strong inhibitory activity against Streptococcus mutans SrtA, with IC50 values of 46.1 and 41.8 μM, respectively, but did not affect cell viability. The onset and magnitude of inhibition of saliva-induced aggregation in S. mutans treated with compounds 1 and 4 were comparable to the behavior of a srtA-deletion mutant without treatment.
Abstract
Seven flavonoids were isolated from Spatholobus suberectus via repetitive column chromatography and high-performance liquid chromatography. The chemical structures of these compounds were identified by spectroscopic analysis and comparison with values reported in the literature. Among the flavonoids tested, 7-hydroxy-6-methoxyflavanone (1) and formononetin (4) exhibited strong inhibitory activity against Streptococcus mutans SrtA, with IC50 values of 46.1 and 41.8 μM, respectively, but did not affect cell viability. The onset and magnitude of inhibition of saliva-induced aggregation in S. mutans treated with compounds 1 and 4 were comparable to the behavior of a srtA-deletion mutant without treatment.
참고문헌 (20)
- Hendrickx AP, "Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds", Nat. Rev. Microbiol, 9, pp.166~176 (2011)
- Jonsson IM, "The role of Staphylococcus aureus sortase A and sortase B in murine arthritis", Microbes Infect, 5, pp.775~780 (2003)
- Clancy KW, "Sortase transpeptidases: insights into mechanism, substrate specificity, and inhibition", Biopolymers, 94, pp.385~396 (2010)
- Selwitz RH, "Dental caries", Lancet, 369, pp.51~59 (2007)
- Lee SF, "Roles of sortase in surface expression of the major protein adhesion P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans", Infect. Immun, 71, pp.676~681 (2003)
- Lévesque CM, "Involvement of sortase anchoring of cell wall proteins in biofilm formation by Streptococcus mutans", Infect. Immun, 73, pp.3773~3777 (2005)
- 조현주, "Spatholobus suberectus Dunn. constituents inhibit sortase A and Staphylococcus aureus cell clumping to fibrinogen", Archives of Pharmacal Research, 40(4), pp.518~523 (2017), 대한약학회
- Yoon JS, "Flavonoids from Spatholobus suberectus", Arch. Pharm. Res, 27, pp.589~592 (2004)
- Abreu Matos FJ, "Flavonoids from Dalbergia ecastophyllum", Phytochemistry, 14, pp.825~826 (1975)
- Braga de Oliveira A, "Flavonoids and coumarins from Platymiscium praecox", Phytochemistry, 11, pp.3515~3519 (1972)
- Bradbury RB, "The chemistry of subterranean clover. Part I. Isolation of formononetin and genistein", J. Chem. Soc, 1951, pp.3447~3449 (1951)
- Bevan CWL, "West African timbers. Part XIII. Extracts from the heartwood of Amphimas pterocarpoides and Pterocarpus erinacesus", J. Chem. Soc. C, 1966, pp.509~510 (1966)
- King FE, "The chemistry of extractives from hardwoods. Part IX. Constituents of the heartwood of Ferreirea spectabilis", J. Chem. Soc, 1952, pp.4580~4584 (1952)
- Keen NT, "Biosynthesis of hydroxyphaseollin and related isoflavanoids in diseaseresistant soybean hypocotyls", Phytochemistry, 11, pp.1031~1039 (1972)
- LEE,KI-YOUNG, "Expression of Sortase, a Transpeptidase for Cell Wall Sorting Reaction, from Staphylococcus aureus ATCC 6538p in Escherichia coli", Journal of Microbiology and Biotechnology, 12(3), pp.530~533 (2002), 한국미생물·생명공학회
- Ton-That H, "Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif", Proc. Natl. Acad. Sci. USA, 96, pp.12424~12429 (1999)
- Hu P, "Curcumin inhibits the sortase A activity of the Streptococcus mutans UA159", Appl. Biochem. Biotechnol, 171, pp.396~402 (2013)
- Kim SH, "Inhibition of the bacterial surface protein anchoring transpeptidase sortase by isoquinoline alkaloids", Biosci. Biotechnol. Biochem, 68, pp.421~424 (2004)
- Mazmanian SK, "Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections", Proc. Natl. Acad. Sci. USA, 97, pp.5510~5515 (2000)
- Frankel BA, "Vinyl sulfones: inhibitors of SrtA, a transpeptidase required for cell wall protein anchoring and virulence in Staphylococcus aureus", J. Am. Chem. Soc, 126, pp.3404~3405 (2004)