Antibacterial and Anti-Glucosyltransferase Activity of Verbascum speciosum Against Cariogenic Streptococci
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Objectives: Dental caries is a prevalent chronic human infection worldwide and several plants have shown anticariogenic properties through antibacterial activity against oral pathogens. The present study aimed to assess anticariogenic activity of Verbascum speciosum, in search of novel agents for the prevention and treatment of dental caries. Methods: Hydro-alcoholic extracts from flowers and total aerial parts of the plant were prepared by maceration. Antibacterial activity of the extracts against Streptococcus mutans (ATCC 35668) and Streptococcus sobrinus (ATCC 27607) was investigated by agar diffusion and microdilution techniques. Inhibitory concentration-fifty values of the flowers’ extract against Streptococcus mutans glucosyltransferase enzymes were determined. The total flavonoid content of the extracts was determined using an aluminum chloride reaction. Results: Verbascum speciosum flowers’ extract showed significantly higher flavonoid content and antibacterial activity; with minimum inhibitory concentrations of 100 and 200 µg/mL for Streptococcus mutans and Streptococcus sobrinus, respectively. The extract inhibited the synthesis of glucan by cell-associated and extracellular glucosyltransferase enzymes in a dose-dependent manner with higher activity against the extracellular enzyme. Conclusion: This study indicated effective anticariogenic activity of Verbascum speciosum flowers extract. This extract can be considered as an alternative to current anticaries therapies or an additive to dental care products.
Abstract
Objectives: Dental caries is a prevalent chronic human infection worldwide and several plants have shown anticariogenic properties through antibacterial activity against oral pathogens. The present study aimed to assess anticariogenic activity of Verbascum speciosum, in search of novel agents for the prevention and treatment of dental caries. Methods: Hydro-alcoholic extracts from flowers and total aerial parts of the plant were prepared by maceration. Antibacterial activity of the extracts against Streptococcus mutans (ATCC 35668) and Streptococcus sobrinus (ATCC 27607) was investigated by agar diffusion and microdilution techniques. Inhibitory concentration-fifty values of the flowers’ extract against Streptococcus mutans glucosyltransferase enzymes were determined. The total flavonoid content of the extracts was determined using an aluminum chloride reaction. Results: Verbascum speciosum flowers’ extract showed significantly higher flavonoid content and antibacterial activity; with minimum inhibitory concentrations of 100 and 200 µg/mL for Streptococcus mutans and Streptococcus sobrinus, respectively. The extract inhibited the synthesis of glucan by cell-associated and extracellular glucosyltransferase enzymes in a dose-dependent manner with higher activity against the extracellular enzyme. Conclusion: This study indicated effective anticariogenic activity of Verbascum speciosum flowers extract. This extract can be considered as an alternative to current anticaries therapies or an additive to dental care products.
참고문헌 (35)
- Wu CY, "Inhibitory effects of tea catechin epigallocatechin-3-gallate against biofilms formed from Streptococcus mutans and a probiotic lactobacillus strain", Arch Oral Biol, 94, pp.69~77 (2018)
- Yang Y, "Effect of LongZhang Gargle on biofilm formation and acidogenicity of Streptococcus mutans in vitro", Biomed Res Int, 2016, pp.5829823~ (2016)
- Law V, "Factors influencing oral colonization of mutans streptococci in young children", Aust Dent J, 52(2), pp.93~100 (2007)
- Nishikawara F, "Correlation of cariogenic bacteria and dental caries in adults", J Oral Sci, 48(4), pp.245~251 (2006)
- Bhagavathy S, "Identification of glucosyl transferase inhibitors from Psidium guajava against Streptococcus mutans in dental caries", J Tradit Complement Med, 9(2), pp.124~137 (2018)
- Veloz JJ, "Polyphenol-rich extract from propolis reduces the expression and activity of Streptococcus mutans glucosyltransferases at subinhibitory concentrations", Biomed Res Int, 2016, pp.4302706~ (2016)
- Hamada S, "Purification and characterization of cell-associated glucosyltransferase synthesizing water-insoluble glucan from serotype C Streptococcus mutans", J Gen Microbiol, 135(Pt 2), pp.335~344 (1989)
- Dorman HJ, "Antimicrobial agents from plants : antibacterial activity of plant volatile oils", J Appl Microbiol, 88(2), pp.308~316 (2000)
- Jazaeri M, "Cariostatic effect of green tea in comparison with common anticariogenic agents : an in vitro study", J Dent Res Dent Clin Dent Prospects, 9(1), pp.44~48 (2015)
- Al-Bayati FA, "In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogens in Iraq", Turk J Biol, 32(1), pp.57~62 (2008)
- Karavelioğulları FA, "Verbascum eskisehirensis sp. nov. (Scrophulariaceae)from central Anatolia, Turkey", Nord J Bot, 27(3), pp.222~227 (2009)
- Sotoodeh A, "Verbascum shahsavarensis(Scrophulariaceae), a new species for flora of Iran", Phytotaxa, 203(1), pp.76~80 (2015)
- Escobar FM, "Antiviral effect and mode of action of methanolic extract of Verbascum thapsus L. on pseudorabies virus(strain RC", 79), 2012, pp.26(17), 1621~1625
- Amirnia R, "Antimicrobial activity of Verbascum speciosum against three bacteria strains", Fresenius Environ Bull, 20(3), pp.690~693 (2011)
- Karamian R, "Total phenolic content, antioxidant and antibacterial activities of three verbascum species from Iran", JMPB, 2(1), pp.43~51 (2013)
- Dastan D, "Protective effects of Allium hirtifolium Boiss extract on cadmium-induced renal failure in rats", Environ Sci Pollut Res Int, 26(18), pp.18886~18892 (2019)
- Amidi N, "Effect of green tea on inflammation and oxidative stress in cisplatin-induced experimental liver function", J Herbmed Pharmacol, 5(3), pp.99~102 (2016)
- Cockerill F, "Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement" (2011), Clinical and Laboratory Standards Institute
- Shimamura A, "Purification and properties of Streptococcus mutans extracellular glucosyltransferase", Biochim Biophys Acta, 702(1), pp.72~80 (1982)
- Kerdar T, "Phytochemical and biological studies of Scrophularia striata from Ilam", Jundishapur J Nat Pharm Prod, 13(3), pp.e62705~ (2018)
- Dulger G, "Antimicrobial potential of the leaves of common mullein(Verbascum thapsus L., Scrophulariaceae)on microorganisms isolated from urinary tract infections", J Med Plants Stud, 3(2), pp.86~89 (2015)
- Prakash V, "Studies on antibacterial activity of Verbascum Thapsus", J Med Plants Stud, 4(3), pp.101~103 (2016)
- Barbour EK, "Screening of selected indigenous plants of Lebanon for antimicrobial activity", J Ethnopharmacol, 93(1), pp.1~7 (2004)
- Chen H, "Flavonoid baicalein suppresses oral biofilms and protects enamel hardness to combat dental caries", Int J Mol Sci, 23(18), pp.10593~ (2022)
- Jamshidi-Kia F, "Iranian species of Verbascum : a review of botany, phytochemistry, and pharmacological effects", Toxin Rev, 38(4), pp.255~262 (2019)
- Asgary S, "Improvement of hypertension, endothelial function and systemic inflammation following short-term supplementation with red beet(Beta vulgaris L. )juice : a randomized crossover pilot study", J Hum Hypertens, 30(10), pp.627~632 (2016)
- Bagheri Zahra, "The Effect of Comfrey on Enoxaparin-Induced Bruise in Patients with Acute Coronary Syndrome: A Randomised Clinical Trial", Journal of Pharmacopuncture, 24(4), pp.196~205 (2021), 대한약침학회[DOI]
- Francis G, "The biological action of saponins in animal systems : a review", Br J Nutr, 88(6), pp.587~605 (2002)
- Okasha YM, "The untargeted phytochemical profile of Verbascum thapsus L. with potent antiviral, antibacterial and anticancer activities", South Afr J Bot, 156, pp.334~341 (2023)
- Amini S, "Phenolic and flavonoid compounds and antioxidant activity in flowers of nine endemic Verbascum species from Iran", J Sci Food Agric, 102(8), pp.3250~3258 (2022)
- Cushnie TP, "Antimicrobial activity of flavonoids", Int J Antimicrob Agents, 27(2), pp.181~ (2006)
- Ferrazzano GF, "Plant polyphenols and their anti-cariogenic properties : a review", Molecules, 16(2), pp.1486~1507 (2011)
- de Araújo JSC, "Antibacterial activity against cariogenic bacteria and cytotoxic and genotoxic potential of Anacardium occidentale L. and Anadenanthera macrocarpa(Benth. )Brenan extracts", Arch Oral Biol, 85, pp.113~119 (2018)
- Koo H, "Influence of apigenin on gtf gene expression in Streptococcus mutans UA159", Antimicrob Agents Chemother, 50(2), pp.542~546 (2006)
- Sakanaka S, "Inhibitory effects of green tea polyphenols on glucan synthesis and cellular adherence of cariogenic Streptococci", Agric Biol Chem, 54(11), pp.2925~2929 (1990)
