Inhibitory Effects of Streptomyces sp. MBTH32 Metabolites on Sortase A and Sortase A-Mediated Cell Clumping of Staphylococcus aureus to Fibrinogen
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Sortase A (SrtA), a type of transpeptidase responsible for anchoring surface proteins to the peptidoglycan cell wall, is important in the virulence of gram-positive bacteria. Three compounds were isolated from marine-derived Streptomyces sp. MBTH32 using various chromatography techniques. The structures of these compounds were determined based on spectroscopic data and comparisons with previously reported data. Among the metabolites tested, lumichrome showed strong inhibitory activity against Staphylococcus aureus SrtA without affecting cell viability. The results of cell clumping activity assessment suggest the potential for using this compound to treat S. aureus infection by inhibiting SrtA activity.
Abstract
Sortase A (SrtA), a type of transpeptidase responsible for anchoring surface proteins to the peptidoglycan cell wall, is important in the virulence of gram-positive bacteria. Three compounds were isolated from marine-derived Streptomyces sp. MBTH32 using various chromatography techniques. The structures of these compounds were determined based on spectroscopic data and comparisons with previously reported data. Among the metabolites tested, lumichrome showed strong inhibitory activity against Staphylococcus aureus SrtA without affecting cell viability. The results of cell clumping activity assessment suggest the potential for using this compound to treat S. aureus infection by inhibiting SrtA activity.
참고문헌 (30)
- Tortorella E, "Antibiotics from deep-sea microorganisms:current discoveries and perspectives", Mar. Drugs, 16, pp.E355~ (2018)
- Tyler PA, "Ecosystems of the deep oceans", pp.1~4 (2003), Elsevier Science
- Wright PC, "Piezotolerance as a metabolic engineering tool for the biosynthesis of natural products", Biomol. Eng, 20, pp.325~331 (2003)
- Bull AT, "Search and discovery strategies for biotechnology : the paradigm shift", Microbiol. Mol. Biol. Rev, 64, pp.573~606 (2000)
- Blunt JW, "Marine natural products", Nat. Prod. Rep, 35, pp.8~53 (2018)
- Braña AF, "Branimycins B and C, antibiotics produced by the abyssal actinobacterium Pseudonocardia carboxydivorans M-227", J. Nat. Prod, 80, pp.569~573 (2017)
- Le TC, "Ansalactams B–D illustrate further biosynthetic plasticity within the ansamycin pathway", Org. Lett, 18, pp.2256~2259 (2016)
- Song Y, "Cyclic hexapeptides from the deep South China Sea-derived Streptomyces scopuliridis SCSIO ZJ46 active against pathogenic gram-positive bacteria", J. Nat. Prod, 77, pp.1937~1941 (2014)
- Pan HQ, "New spirotetronate antibiotics, lobophorins H and I, from a South China Sea-derived Streptomyces sp. 12A35", Mar. Drugs, 11, pp.3891~3901 (2013)
- Cossart P, "Sortase, a universal target for therapeutic agents against Gram-positive bacteria?", Proc. Natl. Acad. Sci. USA, 97, pp.5013~5015 (2000)
- Mazmanian SK, "Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections", Proc. Natl. Acad. Sci. USA, 97, pp.5510~5515 (2000)
- Wesson CA, "Staphylococcus aureus A gr a nd S ar g lobal regulators influence internalization and induction of apoptosis", Infect. Immun, 66, pp.5238~5243 (1998)
- Mazmanian SK, "An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis", Proc. Natl. Acad. Sci. USA, 99, pp.2293~2298 (2002)
- Mazmanian SK, "Passage of heme-iron across the envelope of Staphylococcus aureus", Science, 299, pp.906~909 (2003)
- Frankel BA, "Vinyl sulfones : inhibitors of SrtA, a transpeptidase required for cell wall protein anchoring and virulence in Staphylococcus aureus", J. Am. Chem. Soc, 126, pp.3404~3405 (2004)
- Sibbald MJ, "Partially overlapping substrate specificities of staphylococcal group A sortases", Proteomics, 12, pp.3049~3062 (2012)
- Cascioferro S, "Sortase A inhibitors : recent advances and future perspectives", J. Med. Chem, 58, pp.9108~9123 (2015)
- Kang H, "Isolation of microbial antibiotics from a marine ascidian of the genus Didemnum", J. Org. Chem, 61, pp.1543~1546 (1996)
- Martínez-Luis S, "Antitrypanosomal alkaloids from the marine bacterium Bacillus pumilus", Molecules, 17, pp.11146~11155 (2012)
- Andrioli WJ, "Isolation and characterization of 2pyridone alkaloids and alloxazines from Beauveria bassiana", Nat. Prod. Res, 31, pp.1920~1929 (2017)
- LEE,KI-YOUNG, "Expression of Sortase, a Transpeptidase for Cell Wall Sorting Reaction, from Staphylococcus aureus ATCC 6538p in Escherichia coli", Journal of Microbiology and Biotechnology, 12(3), pp.530~533 (2002), 한국미생물·생명공학회
- Oh KB, "Discovery of diarylacrylonitriles as a novel series of small molecule sortase A inhibitors", J. Med. Chem, 47, pp.2418~2421 (2004)
- Hu P, "Curcumin inhibits the sortase A activity of the Streptococcus mutans UA 159", Appl. Biochem. Biotechnol, 171, pp.396~402 (2013)
- Kim SH, "Inhibition of the bacterial surface protein anchoring transpeptidase sortase by isoquinoline alkaloids", Biosci. Biotechnol. Biochem, 68, pp.421~424 (2004)
- Jonsson M, "The role of Staphylococcus aureus sortase A and sortase B in murine arthritis", Microbes Infect, 5, pp.775~780 (2003)
- Weinstein MP, "Methods for Dilution Antimicrobial Susceptibility Test for Bacteria That Grow aerobically", Clinical and Laboratory Standards Institute, 15~50 (2018)
- Lineweaver H, "The determination of enzyme dissociation constants", J. Am. Chem. Soc, 56, pp.658~666 (1934)
- Alksne LE, "Bacterial virulence as a target for antimicrobial chemotherapy", Curr. Opin. Biotechnol, 11, pp.625~636 (2000)
- Zhang B, "Chalcone attenuates Staphylococcus aureus virulence by targeting sortase A and alpha-hemolysin", Front. Microbiol, 8, pp.1715~ (2017)
- Weiss WJ, "Effect of srtA and srtB gene expression on the virulence of Staphylococcus aureus in animal models of infection", J. Antimicrob. Chemother, 53, pp.480~486 (2004)
