Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans
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Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and TNF-α) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.
Abstract
Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and TNF-α) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.
참고문헌 (50)
- Wang Z, "Dietary Enterococcus faecium NCIMB 10415 and zinc oxide stimulate immune reactions to trivalent influenza vaccination in pigs but do not affect virological response upon challenge infection", PLoS One, 9, pp.e87007~ (2014)[DOI]
- Tsai Y-T, "The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits", Appl. Microbiol. Biotechnol., 96, pp.853~862 (2012)[DOI]
- Signorini M, "Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria", Res. Vet. Sci., 93, pp.250~258 (2012)[DOI]
- Brestoff JR, "Commensal bacteria at the interface of host metabolism and the immune system", Nat. Immunol., 14, pp.676~684 (2013)[DOI]
- Hooper LV, "Interactions between the microbiota and the immune system", Science, 336, pp.1268~1273 (2012)[DOI]
- Cotter PD, "Bacteriocins - a viable alternative to antibiotics?", Nat. Rev. Microbiol., 11, pp.95~105 (2013)[DOI]
- Salim H, "Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens", Poult. Sci., 92, pp.2084~2090 (2013)[DOI]
- Cao G, "Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88", Poult. Sci., 92, pp.2949~2955 (2013)[DOI]
- Huang Y, "Effect of orally administered Enterococcus faecium EF1 on intestinal cytokines and chemokines production of suckling piglets", Pak. Vet. J., 32, pp.81~84 (2012)
- Scharek L, "Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets", Vet. Immunol. Immunopathol., 105, pp.151~161 (2005)[DOI]
- Valeriano VD, "In vitro evaluation of the mucin-adhesion ability and probiotic potential of Lactobacillus mucosae LM1", J. Appl. Microbiol., 117, pp.485~497 (2014)[DOI]
- Tellez G, "Probiotics", direct fed microbials for Salmonella control in poultry, 2012, pp.45, 628~633
- Zhang R, "Host-microbe interactions in Caenorhabditis elegans", ISRN Microbiol, 2013, pp.356451~ (2013)
- Komura T, "Mechanism underlying prolongevity induced by bifidobacteria in Caenorhabditis elegans", Biogerontology, 14, pp.73~87 (2013)[DOI]
- Ikeda T, "Influence of lactic acid bacteria on longevity of Caenorhabditis elegans and host defense against Salmonella enterica serovar enteritidis", Appl. Environ. Microbiol., 73, pp.6404~6409 (2007)[DOI]
- Lee J, "Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: immune modulation and longevity", Int. J. Food Microbiol., 148, pp.80~86 (2011)[DOI]
- Ye M, "The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed", R. Soc. Open Sci., 4, pp.171012~ (2017)[DOI]
- Messaoudi S, "Identification of lactobacilli residing in chicken ceca with antagonism against Campylobacter", Int. Microbiol., 14, pp.103~110 (2011)
- Anandharaj M, "Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property", J. Biosci. Bioeng., 118, pp.153~159 (2014)[DOI]
- Thompson JD, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22, pp.4673~4680 (1994)[DOI]
- Bautista-Gallego J, "Screening of lactic acid bacteria isolated from fermented table olives with probiotic potential", Food Res. Int., 50, pp.135~142 (2013)[DOI]
- Ricciardi A, "Behaviour of lactic acid bacteria populations in Pecorino di Carmasciano cheese samples submitted to environmental conditions prevailing in the gastrointestinal tract: evaluation by means of a polyphasic approach", Int. J Food Microbiol., 179, pp.64~71 (2014)[DOI]
- Argyri AA, "Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests", Food Microbiol, 33, pp.282~291 (2013)[DOI]
- Hwang JH, "Intestinal immune system-modulating activity through Peyer's patch of flavonoid glycoside purified from Citrus unshiu peel", J. Food Biochem., 37, pp.151~160 (2013)[DOI]
- Li N, "Live and heat-killed Lactobacillus rhamnosus GG: effects on proinflammatory and anti-inflammatory cytokines", chemokines in gastrostomy-fed infant rats, 2009, pp.66, 203~207
- Ranke J, "Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays", Ecotoxicol. Environ. Saf., 58, pp.396~404 (2004)[DOI]
- Yoon T J, "Innate immune stimulation of exo-polymers prepared from Cordyceps sinensis by submerged culture", Appl. Microbiol. Biotechnol., 80, pp.1087~1093 (2008)[DOI]
- Thomas TP, "Folate-targeted nanoparticles show efficacy in the treatment of inflammatory arthritis", Arthritis Rheum, 63, pp.2671~2680 (2011)[DOI]
- Stiernagle T, "Maintenance of C. elegans;C. elegans: A Practical Approach", pp.51~67 (1999), Oxford University Press
- Gruber J, "Deceptively simple but simply deceptive- Caenorhabditis elegans lifespan studies: considerations for aging and antioxidant effects", FEBS Lett, 583, pp.3377~3387 (2009)[DOI]
- Wu D, "Visualizing hidden heterogeneity in isogenic populations of C. elegans", Exp. Gerontol., 41, pp.261~270 (2006)[DOI]
- Burdine R D, "Easy RNA isolation from C. elegans: a TRIZOL based method", Worm Breed. Gaz., 14, pp.10~ (1996)
- Greer E L, "An AMPK-FOXO pathway mediates longevity induced by a novel method of dietary restriction in C. elegans", Curr. Biol., 17, pp.1646~1656 (2007)[DOI]
- Untergasser A, "Primer3 - new capabilities and interfaces", Nucleic Acids Res, 40, pp.e115~ (2012)[DOI]
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the <TEX>$2^{-{\Delta}{\Delta}CT}$<", TEX> method, 2001, pp.25, 402~408
- Yang Zhao, "Lactobacillus salivarius Strain FDB89 Induced Longevity in Caenorhabditis elegans by Dietary Restriction", The Journal of Microbiology, 51(2), pp.183~188 (2013), 한국미생물학회[DOI]
- Joint FAO, "Guidelines for the evaluation of probiotics in foods", pp.2002, WHO Working Group
- Zhao T, "Histone deacetylase III as a potential therapeutic target for the treatment of lethal sepsis", J. Trauma Acute Care Surg., 77, pp.913~919 (2014)[DOI]
- Mishra K, "Aqueous extract of Rhodiola imbricata rhizome stimulates proinflammatory mediators via phosphorylated <TEX>$l{\kappa}B$<", TEX> and transcription factor nuclear factor-<TEX>${\kappa}B$<, Immunopharmacol. Immunotoxicol., pp.2006, 28[DOI]
- Turvey SE, "Innate immunity", J. Allergy Clin. Immunol., 125, pp.S24~S32 (2010)[DOI]
- Schepetkin IA, "Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha", Int. Immunopharmacol., 8, pp.1455~1466 (2008)[DOI]
- Renshaw M, "Cutting edge: impaired Toll-like receptor expression and function in aging", J. Immunol., 169, pp.4697~4701 (2002)[DOI]
- Radtke S, "Cross-regulation of cytokine signalling: proinflammatory cytokines restrict IL-6 signalling through receptor internalisation and degradation", J. Cell Sci., 123, pp.947~959 (2010)[DOI]
- Niu X, "Esculin exhibited anti-inflammatory activities in vivo and regulated TNF-<TEX>${\alpha}$<", TEX> and IL-6 production in LPS-stimulated mouse peritoneal macrophages in vitro through MAPK pathway, 2015, pp.29, 779~786
- Grompone G, "Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans", PLoS One, 7, pp.e52493~ (2012)[DOI]
- Kurz CL, "Caenorhabditis elegans: an emerging genetic model for the study of innate immunity", Nat. Rev. Genet., 4, pp.380~390 (2003)
- Millet AC, "Immunity in Caenorhabditis elegans", Curr. Opin. Immunol., 16, pp.4~9 (2004)[DOI]
- Okuyama T, "The ERK-MAPK pathway regulates longevity through SKN-1 and insulin-like signaling in Caenorhabditis elegans", J. Biol. Chem., 285, pp.30274~30281 (2010)[DOI]
- Engelmann I, "Innate immunity in C. elegans;Invertebrate Immunity", Advances in Experimental Medicine and Biology, 708, pp.105~121 (2010)
- Araujo TF, "The genus Enterococcus as probiotic: safety concerns", Braz. Arch. Biol. Technol., 56, pp.457~466 (2013)[DOI]
