Enterococcus faecium FC-K Derived from Kimchi Is a Probiotic Strain That Shows Anti-Allergic Activity
3
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability of Enterococcus faecium FC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages with E. faecium FC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4, E. faecium FC-K enhanced the ability of T cells to produce interferon (IFN)-γ. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receiving E. faecium FC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased in E. faecium FC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of IFN-γ, with no effect on IL-4, in E. faecium FC-Ktreated mice. These observations provide the evidence that E. faecium FC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.
Abstract
A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability of Enterococcus faecium FC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages with E. faecium FC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4, E. faecium FC-K enhanced the ability of T cells to produce interferon (IFN)-γ. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receiving E. faecium FC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased in E. faecium FC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of IFN-γ, with no effect on IL-4, in E. faecium FC-Ktreated mice. These observations provide the evidence that E. faecium FC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.
참고문헌 (25)
- Okada H, "The ‘hygiene hypothesis’ for autoimmune and allergic diseases: an update", Clin. Exp. Immunol, 160, pp.1~9 (2010)
- Strachan DP., "Hay fever, hygiene, and household size", Brit. Med. J, 299, pp.1259~1260 (1989)
- Fahy JV., "Type 2 inflammation in asthma - present in most, absent in many", Nat. Rev. Immunol, 15, pp.57~65 (2015)
- Kaiko GE, "Immunological decision-making: how does the immune system decide to mount a helper T-cell response?", Immunology, 123, pp.326~338 (2008)
- Kay AB., "Allergy and allergic diseases. First of two parts", N. Engl. J . Med, 344, pp.30~37 (2001)
- Lozupone CA, "Diversity, stability and resilience of the human gut microbiota", Nature, 489, pp.220~230 (2012)
- Vieira AT, "The role of probiotics and prebiotics in inducing gut immunity", Front. Immunol, 4, pp.445~ (2013)
- Jung JY, "Metagenomic analysis of kimchi, a traditional Korean fermented food", Appl. Environ. Microbiol, 77, pp.2264~2274 (2011)
- Kang H, "Induction of Th1 cytokines by Leuconostoc mesenteroides subsp. mesenteroides (KCTC 3100) under Th2-type conditions and the requirement of NF-kappaB and p38", JNK, 2009, pp.46, 283~289
- Kang H, "Leuconostoc citreum HJ-P4 (KACC 91035) regulates immunoglobulin E in an ovalbumin-induced allergy model and induces interleukin-12 through nuclear factor-kappa B and p38", c-Jun N-terminal kinases signaling in macrophages, 2009, pp.53, 331~339
- Kang H, "Immunomodulatory effects of Leuconostoc citreum EFEL2061 isolated from kimchi, a traditional Korean food, on the Th2 type-dominant immune response in vitro and in vivo", J Funct. Foods, 20, pp.79~87 (2016)
- Ha D, "Identification of bacteriocinproducing lactic acid bacteria from kimchi and partial characterization of their bacteriocin", J. Microbiol. Biotechnol, 4, pp.305~315 (1994)
- MOON, GI-SEONG, "Isolation, Identification, and Characterizationof a Bacteriocin-Producing Enterococcus sp. FromKimchi and Its Application to Kimchi Fermentation", Journal of Microbiology and Biotechnology, 14(5), pp.924~931 (2004), 한국미생물·생명공학회
- Miettinen M, "Lactobacilli and streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells", Infect. Immun, 66, pp.6058~6062 (1998)
- Mansour NM, "Isolation of Enterococcus faecium NM113, Enterococcus faecium NM213 and Lactobacillus casei NM512 as novel probiotics with immunomodulatory properties", Microbiol. Immunol, 58, pp.559~569 (2014)
- Noben-Trauth N, "Conventional, naive CD4+ T cells provide an initial source of IL-4 during Th2 differentiation", J. Immunol, 165, pp.3620~3625 (2000)
- Gould HJ, "IgE in allergy and asthma today", Nat. Rev. Immunol, 8, pp.205~217 (2008)
- Shang XZ, "Regulation of antigen-specific versus by-stander IgE production after antigen sensitization", Cell. Immunol, 229, pp.106~116 (2004)
- Pene J, "IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2", Proc. Natl. Acad. Sci. USA, 85, pp.6880~6884 (1988)
- Karimi K, "Lactobacillus reuteri-induced regulatory T cells protect against an allergic airway response in mice", Am. J. Respir. Crit. Care Med, 179, pp.186~193 (2009)
- Feleszko W, "Probiotic-induced suppression of allergic sensitization and airway inflammation is associated with an increase of T regulatory-dependent mechanisms in a murine model of asthma", Clin. Exp. Allergy, 37, pp.498~505 (2007)
- Kwon HK, "Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders", Proc. Natl. Acad. Sci. USA, 107, pp.2159~2164 (2010)
- Yoshida T, "An increased number of CD4+CD25+cells induced by an oral administration of Lactobacillus plantarum NRIC0380 are involved in antiallergic activity", Int. Arch. Allergy Immunol, 162, pp.283~289 (2013)
- Ozdemir C, "T regulatory cells and their counterparts: masters of immune regulation", Clin. Exp. Allergy, 39, pp.626~639 (2009)
- Ling EM, "Relation of CD4+CD25+ regulatory Tcell suppression of allergen-driven T-cell activation to atopic status and expression of allergic disease", Lancet, 363, pp.608~615 (2004)