A Simple Enzymatic Method for Quantitation of 2’-Fucosyllactose
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2’-Fucosyllactose (2’-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2’-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2’-FL in a microplate format. The method involves two steps: (i) release of L-fucose from 2’-FL by α-(1-2,3,4,6)-L-fucosidase and (ii) measurement of NADPH formed during the oxidation of L-fucose by L-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2’-FL in 50 min using a 96- well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.
Abstract
2’-Fucosyllactose (2’-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2’-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2’-FL in a microplate format. The method involves two steps: (i) release of L-fucose from 2’-FL by α-(1-2,3,4,6)-L-fucosidase and (ii) measurement of NADPH formed during the oxidation of L-fucose by L-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2’-FL in 50 min using a 96- well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.
참고문헌 (23)
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