Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode
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초록
The fungi associated with termites secrete enzymes such as laccase (multi-copper oxidase) that can degrade extracellular wood matrix. Laccase uses molecular oxygen as an electron acceptor to catalyze the degradation of organic compounds. Owing to its ability to transfer electrons from the cathodic electrode to molecular oxygen, laccase has the potential to be a biocatalyst on the surface of the cathodic electrode of a microbial fuel cell (MFC). In this study, a twochamber MFC using the laccase-producing fungus Galactomyces reessii was investigated. The fungus cultured on coconut coir was placed in the cathode chamber, while an anaerobic microbial community was maintained in the anode chamber fed by industrial rubber wastewater and supplemented by sulfate and a pH buffer. The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a 1,000 Ω resistor), power density of 59 mW/m2, and current density of 278 mA/m2, and a 70% increase in half-cell potential. This study demonstrated the capability of laccaseproducing yeast Galactomyces reessii as a biocatalyst on the cathode of the two-chamber lbMFC.
Abstract
The fungi associated with termites secrete enzymes such as laccase (multi-copper oxidase) that can degrade extracellular wood matrix. Laccase uses molecular oxygen as an electron acceptor to catalyze the degradation of organic compounds. Owing to its ability to transfer electrons from the cathodic electrode to molecular oxygen, laccase has the potential to be a biocatalyst on the surface of the cathodic electrode of a microbial fuel cell (MFC). In this study, a twochamber MFC using the laccase-producing fungus Galactomyces reessii was investigated. The fungus cultured on coconut coir was placed in the cathode chamber, while an anaerobic microbial community was maintained in the anode chamber fed by industrial rubber wastewater and supplemented by sulfate and a pH buffer. The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a 1,000 Ω resistor), power density of 59 mW/m2, and current density of 278 mA/m2, and a 70% increase in half-cell potential. This study demonstrated the capability of laccaseproducing yeast Galactomyces reessii as a biocatalyst on the cathode of the two-chamber lbMFC.
참고문헌 (23)
- Logan BE, "Conversion of wastes into bioelectricity and chemicals by using microbial electrochemical technologies", Science, 337, pp.686~690 (2012)[DOI]
- Zhang F, "Microbial desalination cells with ion exchange resin packed to enhance desalination at low salt concentration", J. Memb. Sci., 417, pp.28~33 (2012)
- Sukkasem C, "Development of a UBFC biocatalyst fuel cell to generate power and treat industrial wastewaters", Bioresour. Technol., 146, pp.749~753 (2013)[DOI]
- Pozdnyakova NN, "Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators", Enzyme Microb. Technol., 39, pp.1242~1249 (2006)[DOI]
- Lee H, "Biotechnological procedures to select white rot fungi for the degradation of PAHs", J. Microbiol. Methods, 97, pp.56~62 (2014)[DOI]
- Barton SC, "Electroreduction of O-2 to water at 0.6 V (SHE) at pH 7 on the 'wired' Pleurotus ostreatus laccase cathode", Biosens. Bioelectron., 17, pp.1071~1074 (2002)[DOI]
- Fishilevich S, "Surface display of redox enzymes in microbial fuel cells", J. Am. Chem. Soc., 131, pp.12052~12053 (2009)[DOI]
- Wu C, "A white-rot fungus is used as a biocathode to improve electricity production of a microbial fuel cell", Appl. Energy, 98, pp.594~596 (2012)[DOI]
- Lai CY, "Decolorization of azo dye and generation of electricity by microbial fuel cell with laccase-producing white-rot fungus on cathode", Appl. Energy, 188, pp.392~398 (2017)[DOI]
- Mani P, "Decolourisation of acid orange 7 in a microbial fuel cell with a laccase-based biocathode: influence of mitigating pH changes in the cathode chamber", Enzyme Microb. Technol., 96, pp.170~176 (2017)[DOI]
- Chaijak P, "Phenol removal from palm oil mill effluent using Galactomyces Reessii termite-associated yeast", Pol. J. Environ Stud., 27, pp.39~44 (2018)[DOI]
- Muyzer G, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S ribosomal RNA", Appl. Environ. Microbiol., 59, pp.695~700 (1993)
- de Lillo A, "Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets", Oral Microbiol. Immunol., 21, pp.61~68 (2006)[DOI]
- Mohammadi M, "Treatment of wastewater from rubber industry in Malaysia", Afr. J. Biotechnol., 9, pp.6233~6243 (2010)
- Lai CY, "Enhanced biodecolorization of acid orange 7 and electricity generation in microbial fuel cells with superabsorbent-containing membrane and laccase-based bio-cathode", J. Clean. Prod., 166, pp.381~386 (2017)[DOI]
- Chu BTT, "Metagenomics reveals the impact of wastewater treatment plants on the dispersal of microorganisms and genes in aquatic sediments", Appl. Environ. Microbiol., 84, pp.e02168~17 (2018)
- Sun HH, "Transformation of anaerobic granules into aerobic granules and the succession of bacterial community", Appl. Microbiol. Biotechnol., 101, pp.7703~7713 (2017)[DOI]
- Oh KH, "Paraperlucidibaca baekdonensis gen. nov., sp nov., isolated from seawater", Int. J. Syst. Evol. Microbiol., 61, pp.1382~1385 (2011)[DOI]
- Martins MD, "Proposal of Ancylothrix gen. nov., a new genus of Phormidiaceae (Cyanobacteria, Oscillatoriales) based on a polyphasic approach", Int. J. Syst. Evol. Microbiol., 66, pp.2396~2405 (2016)[DOI]
- Tanikawa D, "Treatment of natural rubber processing wastewater using a combination system of a two-stage up-flow anaerobic sludge blanket and downflow hanging sponge system", Water Sci. Technol., 73, pp.1777~1784 (2016)[DOI]
- Watari T, "Development of a BR-UASB-DHS system for natural rubber processing wastewater treatment", Environ. Technol., 37, pp.459~465 (2016)[DOI]
- Nilsson JM, "Techniques of circuit analysis;Electric Circuits, 9th Ed.", pp.88~143 (2011), Pearson Education
- Morant KV, "Isolation and bioelectrochemical characterization of novel fungal sources with oxidasic activity applied in situ for the cathodic oxygen reduction in microbial fuel cells", Enzyme Microb. Technol., 66, pp.20~27 (2014)[DOI]