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Employing Laccase-Producing Aspergillus sydowii NYKA 510 as a Cathodic Biocatalyst in Self-Sufficient Lighting Microbial Fuel Cell

Yomna K. Abdallah|Alberto T. Estevez|Diaa El Deen M. Tantawy|Ahmad M. Ibraheem|Neveen M. Khalil
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2019.12|Journal of Microbiology and Biotechnology|제29권 제12호|pp. 1861-1872|DOI 10.4014/jmb.1907.07031

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laccasemicrobial fuel cellAspergillus sydowiiself-sufficient lighting design
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초록

In the present work, we isolated and identified Aspergillus sydowii NYKA 510 as the most potent laccase producer. Its medium constituents were optimized to produce the highest possible amount of laccase, which was after 7 days at 31oC and pH 5.2. Banana peel and peptone excelled in inducing laccase production at concentrations of 15.1 and 2.60 g/l, respectively. Addition of copper sulfate elevated enzyme yield to 145%. The fungus was employed in a microbial fuel cell (MFC). The best performance was obtained at 2000 Ω achieving 0.76 V, 380 mAm-2, 160 mWm-2, and 0.4 W. A project to design a self-sufficient lighting unit was implemented by employing a system of 2 sets of 4 MFCs each, connected in series, for electricity generation. A scanning electron microscopy image of A. sydowii NYKA 510 was utilized in algorithmic form generation equations for the design. The mixed patterning and patterned customized mass approach were developed by the authors and chosen for application in the design.

Abstract

In the present work, we isolated and identified Aspergillus sydowii NYKA 510 as the most potent laccase producer. Its medium constituents were optimized to produce the highest possible amount of laccase, which was after 7 days at 31oC and pH 5.2. Banana peel and peptone excelled in inducing laccase production at concentrations of 15.1 and 2.60 g/l, respectively. Addition of copper sulfate elevated enzyme yield to 145%. The fungus was employed in a microbial fuel cell (MFC). The best performance was obtained at 2000 Ω achieving 0.76 V, 380 mAm-2, 160 mWm-2, and 0.4 W. A project to design a self-sufficient lighting unit was implemented by employing a system of 2 sets of 4 MFCs each, connected in series, for electricity generation. A scanning electron microscopy image of A. sydowii NYKA 510 was utilized in algorithmic form generation equations for the design. The mixed patterning and patterned customized mass approach were developed by the authors and chosen for application in the design.

참고문헌 (44)

  1. Rahimnejad M, "Microbial fuel cell as new technology for bioelectricity generation : a review", Alexandria Eng. J, 54, pp.745~756 (2015)
  2. Rahimnejad M, "Power generation from organic substrate in batch and continuous flow microbial fuel cell operations", Appl. Energy, 88, pp.3999~4004 (2011)
  3. Santoro C, "Microbial fuel cells: from fundamentals to applications, A review", J. Power Sources, 356, pp.225~244 (2017)
  4. Liu H, "Electricity generation using an aircathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane", Environ. Sci Technol, 38, pp.4040~4046 (2004)
  5. Rahimnejad M, "Low voltage power generation in a biofuel cell using anaerobic cultures", World Appl. Sci. J, 6, pp.1585~1588 (2009)
  6. Najafpour G, "The enhancement of a microbial fuel cell for electrical output using mediators and oxidizing agents", Energy Source, 33, pp.2239~2248 (2011)
  7. Lai CY, "Decolorization of azo dye and generation of electricity by microbial fuel cell with laccase-producing white-rot fungus on cathode", Appl. Energy, 188, pp.392~398 (2017)
  8. Chen GW, "Application of biocathode in microbial fuel cells : cell performance and microbial community", Appl. Microbiol. Biotechnol, 79, pp.379~388 (2008)
  9. Sharma Y, "The variation of power generation with organic substrates in single-chamber microbial fuel cells(SCMFCs)", Bioresour. Technol, 101, pp.1844~1850 (2010)
  10. Kacem SH, "New efficient laccase immobilization strategy using ionic liquids for bio-catalysis and microbial fuel cells applications", J. Chem. Technol. Biotechnol, 93, pp.174~183 (2017)
  11. Dwivedi UN, "Review : structure-function relationship among bacterial, fungal and plant laccases", J. Mol. Catal. B : Enzymatic, 68, pp.117~128 (2011)
  12. Pimprapa Chaijak, "Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode", Journal of Microbiology and Biotechnology, 28(8), pp.1360~1366 (2018), 한국미생물·생명공학회
  13. Barton SC, "Electroreduction of O-2 to water at 0.6 V (SHE) at pH 7 on the ‘wired’ Pleurotus ostreatus laccase cathode", Biosens. Bioelectron, 17, pp.1071~1074 (2002)
  14. Mani P, "Decolourisation of acid orange 7 in a microbial fuel cell with a laccase-based biocathode : influence of mitigating pH changes in the cathode chamber", Enzyme Microb. Technol, 96, pp.170~176 (2017)
  15. Lai CY, "Decolorization of azo dye and generation of electricity by microbial fuel cell with laccase-producing white-rot fungus on cathode", Appl. Energy, 188, pp.392~398 (2017)
  16. Johnson LF, "Methods for Studying Soil Mycoflora: Plant Diseases Relationships", pp.179~ (1960), Burgess Publishing Co.
  17. Moubasher AH, "Soil Fungi in Qatar and other Arab Countries", pp.566~ (1993), The Scientific and Applied Research Centre., University of Qatar
  18. Watanabe T., "Soil and Seed Fungi, Pictorial Atlas of Soil and Seed Fungi. Morphologies of Cultured Fungi and Key to Species" (2002), CRC Press
  19. Olga VKS, "Purification and characterization of the constitutive form of laccase from basidiomycete Coriolus hirsutus and effect of inducers on laccase synthesis", Biotechnol. Appl. Biochem, 28, pp.47~54 (1998)
  20. Das P, "Improved bioavailability and biodegradation of a model poly aromatic hydrocarbon by a biosurfactant producing bacterium of marine origin", Chemosphere, 72, pp.1229~1234 (2008)
  22. Cheng S, "Increased performance of single chamber microbial fuel cells using an improved cathode structure", Electrochem. Commun, 8, pp.489~494 (2006)
  23. Khater DZ, "Microbial diversity structure in acetate single chamber microbial fuel cell for electricity generation", J. Gen. Eng. Biotechnol, 15, pp.127~137 (2017)
  24. Jadhav GS, "Performance of microbial fuel cell subjected to variation in pH, temperature, external load and substrate concentration", Bioresour. Technol, 100, pp.717~723 (2009)
  25. Logan BE, "Microbial Fuel Cells", pp.216~ (2008), John Wiley & Sons
  27. Brijwani K, "Fungal laccases:production, function, and applications in food processing", Enzyme Res, 2010, pp.149748~ (2010)
  28. Ghosh P, "Statistical optimization of laccase production by Aspergillus flavus PUF5 through submerged fermentation using agro-waste as cheap substrate", Acta Biologica Szegediensis, 61, pp.25~33 (2017)
  29. Prajapati HV, "Laccase - a wonder molecule: a review of its properties and applications", Int. J. Pure Appl. Biosci, 6, pp.766~773 (2018)
  30. Bhattacharya SS, "Optimization of laccase production using response surface methodology coupled with differential evolution", New Biotechnol, 28, pp.31~39 (2011)
  31. Kirk PM, "Dictionary of the fungi", pp.68~ (2008), CABI
  32. Cong B, "Isolation, characterization and transcriptome analysis of a novel Antarctic Aspergillus sydowii strain MS-19 as a potential lignocellulosic enzyme source", BMC Microbiol, 17, pp.129~ (2017)
  33. More SS, "Isolation, purification, and characterization of fungal laccase from Pleurotus sp", Enzyme Res, 2011, pp.248735~ (2011)
  34. Pointing SB, "Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture", Mycologia, 92, pp.139~144 (2000)
  35. Hazuchová M, "The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus", Nova Biotechnologica et Chimica, 16, pp.113~123 (2017)
  36. Vivekanand V, "Banana peel: a potential substrate for laccase production by Aspergillus fumigatus VkJ2,4.5 in solid-state fermentation", Appl. Biochem. Biotechnol, 165, pp.204~220 (2011)
  37. Minussi RC, "Potential applications of laccase in the food industry", Trends Food Sci. Technol, 13, pp.205~216 (2002)
  38. Couto R, "Application of solid-state fermentation to ligninolytic enzyme production", Biochem. Eng. J, 36, pp.211~219 (2005)
  39. Pant D, "A review of the substrates used in microbial fuel cells(MFCs)for sustainable energy production", Bioresour. Technol, 101, pp.1533~1543 (2010)
  40. Tanikkula P, "Performance of a membrane-less air-cathode single chamber microbial fuel cell in electricity generation from distillery wastewater", Energy Procedia, 79, pp.646~650 (2015)
  41. Oh SE, "Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies", Water Res, 39, pp.4673~4682 (2005)
  42. Liu H, "Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell", Environ. Sci. Technol, 9, pp.658~662 (2006)
  43. Flimban SGA, "Overview of recent advancements in the microbial fuel cell from fundamentals to applications: Design, major elements, and scalability", Energies, 12, pp.3390~ (2019)
  44. Azuma M, "Catalyst Development of Microbial Fuel Cells for Renewable-Energy Production" (2018)

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