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Inoculation with Indole-3-Acetic Acid- Producing Rhizospheric Rhodobacter sphaeroides KE149 Augments Growth of Adzuki Bean Plants Under Water Stress

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초록

The use of plant growth-promoting rhizobacteria is economically viable and environmentally safe for mitigating various plant stresses. Abiotic stresses such as flood and drought are a serious threat to modern agriculture. In the present study, the indole-3-acetic acid-producing rhizobacterium R. sphaeroides KE149 was selected, and its effects on the growth of adzuki bean plants under flood stress (FS) and drought stress (DS) were investigated. IAA quantification of bacterial pure culture revealed that KE149 produced a significant amount of IAA. Moreover, KE149 inoculation notably decreased stress-responsive endogenous abscisic acid and jasmonic acid and increased salicylic acid in plants under DS and FS. KE149 inoculation also increased proline under DS and methionine under FS. In addition, KE149 inoculation significantly increased the levels of calcium (Ca), magnesium (Mg), and potassium (K) while lowering the sodium (Na) content in the plant shoot under stress. KE149-treated plants had markedly greater root length, shoot length, stem diameter, biomass, and higher chlorophyll content under both normal and stressed conditions. These results suggest that KE149 could be an efficient biofertilizer for mitigating water stress.

Abstract

The use of plant growth-promoting rhizobacteria is economically viable and environmentally safe for mitigating various plant stresses. Abiotic stresses such as flood and drought are a serious threat to modern agriculture. In the present study, the indole-3-acetic acid-producing rhizobacterium R. sphaeroides KE149 was selected, and its effects on the growth of adzuki bean plants under flood stress (FS) and drought stress (DS) were investigated. IAA quantification of bacterial pure culture revealed that KE149 produced a significant amount of IAA. Moreover, KE149 inoculation notably decreased stress-responsive endogenous abscisic acid and jasmonic acid and increased salicylic acid in plants under DS and FS. KE149 inoculation also increased proline under DS and methionine under FS. In addition, KE149 inoculation significantly increased the levels of calcium (Ca), magnesium (Mg), and potassium (K) while lowering the sodium (Na) content in the plant shoot under stress. KE149-treated plants had markedly greater root length, shoot length, stem diameter, biomass, and higher chlorophyll content under both normal and stressed conditions. These results suggest that KE149 could be an efficient biofertilizer for mitigating water stress.

참고문헌 (48)

  1. Hee-La Ryu, "Evaluation of Growth Characteristics and Groundwater Levels for the Growth and Development of Sorghum (Sorghum bicolor L.) and Adzuki bean(Vigna anaularis L.)", 농업생명과학연구, 52(6), pp.13~25 (2018), 농업생명과학연구원[DOI]
  2. Złotek U, "Antioxidant activity of polyphenols of adzuki bean(Vigna angularis)germinated in abiotic stress conditions", Acta Sci. Pol. Technol. Aliment, 14, pp.55~63 (2015)
  3. Yoshida Y, "Salt tolerance in wild relatives of adzuki bean, Vigna angularis (Willd.) Ohwi et Ohashi. Genet. Resour", Crop Evol., 63, pp.627~637 (2016)
  4. Wang W, "Plant responses to drought, salinity and extreme temperatures : towards genetic engineering for stress tolerance", Planta, 218, pp.1~14 (2003)
  5. Lesk C, "Influence of extreme weather disasters on global crop production", Nature, 529, pp.84~87 (2016)
  6. Kim YH, "Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance", Front. Plant Sci., 6, pp.714~ (2015)
  7. Kim YH, "Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance", Front. Plant Sci., 6, pp.714~ (2015)
  8. Sarwat M, "Hormonal signaling to control stomatal movement during drought stress", Plant Gene, 11, pp.143~153 (2017)
  9. Muñoz-Espinoza VA, "Water stress responses of tomato mutants impaired in hormone biosynthesis reveal abscisic acid, jasmonic acid and salicylic acid interactions", Front. Plant Sci, 6, pp.997~ (2015)
  10. Waqas M, "Endophytic infection alleviates biotic stress in sunflower through regulation of defence hormones, antioxidants and functional amino acids", Eur. J. Plant Pathol, 141, pp.803~824 (2015)
  11. Moe LA, "Amino acids in the rhizosphere : from plants to microbes", Am. J. Botany, 100, pp.1692~1705 (2013)
  12. Cyr D, "Accumulation of free amino acids in the shoots and roots of three northern conifers during drought", Tree Physiol, 6, pp.293~303 (1990)
  13. Kang SM, "Gibberellin secreting rhizobacterium, Pseudomonas putida H-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions", Plant Physiol. Biochem, 84, pp.115~124 (2014)
  14. Kang SM, "Plant growth-promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus", J. Plant Interact, 9, pp.673~682 (2014)
  15. Kang SM, "Cucumber performance is improved by inoculation with plant growth-promoting microorganisms", Acta Agriculturae Scandinavica, B—Soil Plant Sci, 65, pp.36~44 (2015)
  16. Cao MJ, "Combining chemical and genetic approaches to increase drought resistance in plants", Nat. Commun, 8, pp.1183~ (2017)
  17. Kang SM, "Indole-3-acetic-acid and ACC deaminase producing Leclercia adecarboxylata MO1 improves Solanum lycopersicum L. growth and salinity stress tolerance by endogenous secondary metabolites regulation", BMC Microbiol, 19, pp.80~ (2019)
  18. Gamalero E, "Bacterial modulation of plant ethylene levels", Plant Physiol, 169, pp.13~22 (2015)
  19. Lee KE, "Isolation and characterization of the high silicate and phosphate solubilizing novel strain Enterobacter ludwigii GAK2 that promotes growth in rice plants", Agron, 9, pp.144~ (2019)
  20. Shahzad R, "Inoculation of abscisic acid-producing endophytic bacteria enhances salinity stress tolerance in Oryza sativa", Environ. Exp. Bot, 136, pp.68~77 (2017)
  21. Khan MA, "Halotolerant Rhizobacterial strains mitigate the adverse effects of NaCl stress in soybean seedlings", BioMed Research International, 2019, pp.9530963~ (2019)
  22. Khan MA, "Halo-tolerant rhizospheric Arthrobacter woluwensis AK1mitigates salt stress and induces physio-hormonal changes and expression of GmST1 and GmLAX3 in soybean", Symbiosis (2018)
  23. Seskar M, "Endogenous methyl salicylate in pathogen-inoculated tobacco plants", Plant Physiol, 116, pp.387~392 (1998)
  24. Arjun Adhikari, "Effect of Silicate and Phosphate Solubilizing Rhizobacterium Enterobacter ludwigii GAK2 on Oryza sativa L. under Cadmium Stress", Journal of Microbiology and Biotechnology, 30(1), pp.118~126 (2020), 한국미생물·생명공학회
  25. Bilal S, "Endophytic Paecilomyces formosus LHL10 augments Glycine max L. adaptation to Ni-contamination through affecting endogenous phytohormones and oxidative stress", Front. Plant Sci., 8, pp.870~ (2017)
  26. Butts TR, "Light quality effect on corn growth as influenced by weed species and nitrogen rate", J. Agric. Sci, 9, pp.15~ (2016)
  27. Bishnu Adhikari, "Resveratrol, total phenolic and flavonoid contents, and antioxidant potential of seeds and sprouts of Korean peanuts", Food Science and Biotechnology, 27(5), pp.1275~1284 (2018), 한국식품과학회[DOI]
  28. Dodd IC, "Microbial amelioration of crop salinity stress", J. Exper. Botany, 63, pp.3415~3428 (2012)
  29. Gimenez-Ibanez S, "How microbes twist jasmonate signaling around their little fingers", Plants, 5(1), pp.9~ (2016)
  30. Baier M, "Oxidative stress and ozone : perception, signalling and response", Plant Cell Environ, 28, pp.1012~1020 (2005)
  31. Singh B, "Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress", Plant Growth Regul, 39, pp.137~141 (2003)
  32. Hayat S, "Growth of tomato(Lycopersicon esculentum)in response to salicylic acid under water stress", J. Plant Inter, 3, pp.297~304 (2008)
  33. Anjum S, "Methyl jasmonate‐induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought", J. Agron. Crop Sci, 197, pp.296~301 (2011)
  34. Kumari G, "Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings", Biol. Plantarum, 50, pp.219~226 (2006)
  35. Kim EH, "Methyl jasmonate reduces grain yield by mediating stress signals to alter spikelet development in rice", Plant Physiol, 149, pp.1751~1760 (2009)
  36. Bharti N, "Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress", 6, pp.34768~ (2016)
  37. Pardo JM, "Biotechnology of water and salinity stress tolerance", Curr. Opin. Biotechnol, 21, pp.185~196 (2010)
  38. Osuagwu G, "The influence of water stress(drought)on the mineral and vitamin potential of the leaves of Ocimum gratissimum(L)", Recent Res. Sci. Technol, 2, pp.2~ (2010)
  39. Smith MR, "Field drought conditions impact yield but not nutritional quality of the seed in common bean (Phaseolus vulgaris L.)", PLoS One, 14, pp.e0217099~ (2019)
  40. Bhaskara GB, "Dynamic proline metabolism : importance and regulation in water limited environments", Front. Plant Sci, 6, pp.484~ (2015)
  41. Agami R, "Exogenous application with plant growth promoting rhizobacteria (PGPR) or proline induces stress tolerance in basil plants (Ocimum basilicum L.) exposed to water stress", Int. J. Environ. Agri. Res., 2, pp.78~ (2016)
  42. Less H, "Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses", Plant Physiol, 147, pp.316~330 (2008)
  43. Waqas M, "Endophytic fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress", Molecules(Basel, Switzerland), 17, pp.10754~10773 (2012)
  44. Ali M, "Effects of bacterial fermentation on the biochemical constituents and antioxidant potential of fermented and unfermented soybeans using probiotic Bacillus subtilis(KCTC 13241)", Molecules(Basel, Switzerland), 22, pp.2200~ (2017)
  45. Cheruiyot EK, "Polyphenols as potential indicators for drought tolerance in tea (Camellia sinensis L.)", Biosci. Biotechnol. Biochem., 71, pp.2190~2197 (2007)
  46. Kang HM, "Antioxidant enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles", J. Agric. Food Chem., 50, pp.513~518 (2002)
  47. Nautiyal CS, "Novel mechanism of modulating natural antioxidants in functional foods : involvement of plant growth promoting rhizobacteria NRRL B-30488", J. Agric. Food Chem, 56, pp.4474~4481 (2008)
  48. Rojas-Tapias D, "Effect of inoculation with plant growthpromoting bacteria(PGPB)on amelioration of saline stress in maize(Zea mays)", Appl. Soil Ecol, 61, pp.264~272 (2012)

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