본문 바로가기
KERD
논문KCI 등재

Enhancement of Drought-Stress Tolerance of Brassica oleracea var. italica L. by Newly Isolated Variovorax sp. YNA59

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류생물학

초록

Drought is a major abiotic factor and has drastically reduced crop yield globally, thus damaging the agricultural industry. Drought stress decreases crop productivity by negatively affecting crop morphological, physiological, and biochemical factors. The use of drought tolerant bacteria improves agricultural productivity by counteracting the negative effects of drought stress on crops. In this study, we isolated bacteria from the rhizosphere of broccoli field located in Daehaw-myeon, Republic of Korea. Sixty bacterial isolates were screened for their growth-promoting capacity, in vitro abscisic acid (ABA), and sugar production activities. Among these, bacterial isolates YNA59 was selected based on their plant growth-promoting bacteria traits, ABA, and sugar production activities. Isolate YNA59 highly tolerated oxidative stress, including hydrogen peroxide (H2O2) and produces superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities in the culture broth. YNA59 treatment on broccoli significantly enhanced plant growth attributes, chlorophyll content, and moisture content under drought stress conditions. Under drought stress, the endogenous levels of ABA, jasmonic acid (JA), and salicylic acid (SA) increased; however, inoculation of YNA59 markedly reduced ABA (877 ± 22 ng/g) and JA (169.36 ± 20.74 ng/g) content, while it enhanced SA levels (176.55 ± 9.58 ng/g). Antioxidant analysis showed that the bacterial isolate YNA59 inoculated into broccoli plants contained significantly higher levels of SOD, CAT, and APX, with a decrease in GPX levels. The bacterial isolate YNA59 was therefore identified as Variovorax sp. YNA59. Our current findings suggest that newly isolated drought tolerant rhizospheric Variovorax sp. YNA59 is a useful stress-evading rhizobacterium that improved droughtstress tolerance of broccoli and could be used as a bio-fertilizer under drought conditions.

Abstract

Drought is a major abiotic factor and has drastically reduced crop yield globally, thus damaging the agricultural industry. Drought stress decreases crop productivity by negatively affecting crop morphological, physiological, and biochemical factors. The use of drought tolerant bacteria improves agricultural productivity by counteracting the negative effects of drought stress on crops. In this study, we isolated bacteria from the rhizosphere of broccoli field located in Daehaw-myeon, Republic of Korea. Sixty bacterial isolates were screened for their growth-promoting capacity, in vitro abscisic acid (ABA), and sugar production activities. Among these, bacterial isolates YNA59 was selected based on their plant growth-promoting bacteria traits, ABA, and sugar production activities. Isolate YNA59 highly tolerated oxidative stress, including hydrogen peroxide (H2O2) and produces superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities in the culture broth. YNA59 treatment on broccoli significantly enhanced plant growth attributes, chlorophyll content, and moisture content under drought stress conditions. Under drought stress, the endogenous levels of ABA, jasmonic acid (JA), and salicylic acid (SA) increased; however, inoculation of YNA59 markedly reduced ABA (877 ± 22 ng/g) and JA (169.36 ± 20.74 ng/g) content, while it enhanced SA levels (176.55 ± 9.58 ng/g). Antioxidant analysis showed that the bacterial isolate YNA59 inoculated into broccoli plants contained significantly higher levels of SOD, CAT, and APX, with a decrease in GPX levels. The bacterial isolate YNA59 was therefore identified as Variovorax sp. YNA59. Our current findings suggest that newly isolated drought tolerant rhizospheric Variovorax sp. YNA59 is a useful stress-evading rhizobacterium that improved droughtstress tolerance of broccoli and could be used as a bio-fertilizer under drought conditions.

참고문헌 (72)

  1. Boyer J, "The US drought of 2012 in perspective : A call to action", Glob. Food Secur, 2, pp.139~143 (2013)
  2. Hussain M, "Improving drought tolerance by exogenous application of glycinebetaine and salicylic acid in sunflower", J. Agron. Crop Sci, 194, pp.193~199 (2008)
  3. Kaya MD, "Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.)", Eur. J. Agron., 24, pp.291~295 (2006)
  4. Nonami H, "Plant water relations and control of cell elongation at low water potentials", J. Plant Res, 111, pp.373~382 (1998)
  5. Zhang X, "Effects of drought stress and water recovery on physiological responses and gene expression in maize seedlings", BMC Plant Biol, 18, pp.68~ (2018)
  6. Daryanto S, "Global synthesis of drought effects on maize and wheat production", PLoS One, 11, pp.e0156362~ (2016)
  7. Farooq M, "Drought stress in grain legumes during reproduction and grain filling", J. Agron. Crop Sci, 203, pp.81~102 (2017)
  8. Ruth Grene, "Oxidative Stress and Acclimation Mechanisms in Plants", The Arabidopsis Book, 1, pp.e0036~ (2002), BioOne[DOI]
  9. Hasanuzzaman M, "Crop Stress and its Management: Perspectives and Strategies", pp.261~315 (2012), Springer Netherlands
  10. Kang S-M, "Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth", Biotechnol. Lett, 31, pp.277~281 (2009)
  11. 강상모, "Gibberellin-Producing Promicromonospora sp. SE188 Improves Solanum lycopersicum Plant Growth and Influences Endogenous Plant Hormones", The Journal of Microbiology, 50(6), pp.902~909 (2012), 한국미생물학회[DOI]
  12. Armada E, "Differential activity of autochthonous bacteria in controlling drought stress in native Lavandula and Salvia plants species under drought conditions in natural arid soil", Microb. Ecol, 67, pp.410~420 (2014)
  13. Kang S-M, "Gibberellin secreting rhizobacterium, Pseudomonas putida H-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions", Plant Physiol. Biochem, 84, pp.115~124 (2014)
  14. Sandhya V, "Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress", Plant Growth Regul, 62, pp.21~30 (2010)
  15. Heidari M, "Plant growth promoting rhizobacteria (PGPR) effect on physiological parameters and mineral uptake in basil (Ociumum basilicm L.) under water stress", ARPN J. Agric. Biol. Sci., 6, pp.6~11 (2011)
  16. Khan MAM, "Effect of water stress and aphid herbivory on flavonoids in broccoli (Brassica oleracea var. italica Plenck)", J. Appl. Bot. Food Qual., 84, pp.178~182 (2011)
  17. Erken O, "Effects of water stress on yield and some quality parameters of Broccoli", 2nd International Symposium on Sustainable Development, pp.231~237 (2010)
  18. Toscano S, "Effect of preharvest abiotic stresses on the accumulation of bioactive compounds in horticultural produce", Front Plant Sci, 10, pp.1212~ (2019)
  19. Kałużewicz A, "Effect of biostimulants on several physiological characteristics and chlorophyll content in broccoli under drought stress and re-watering", Not. Bot. Horti. Agrobo, 45, pp.197~202 (2017)
  20. Munawar A, "Nitric oxide regulates oxidative defense system, key metabolites and growth of broccoli (Brassica oleracea L.) plants under water limited conditions", Sci. Hortic., 254, pp.7~13 (2019)
  21. Ertan Y, "Growth, nutrient uptake, and yield promotion of broccoli by plant growth promoting rhizobacteria with manure", HortScience, 46, pp.932~936 (2011)
  22. Yildirim E, "Potential use of plant growth promoting rhizobacteria in organic broccoli (Brassica oleracea L., var. italica) production", Ecofruit 14th International Conference on Organic Fruit-Growing, pp.227~235 (2010)
  23. El-Nemr M, "Enhancement of growth and production of broccoli crop using bionutritional foliar compound", Aust. J. Basic Appl. Sci, 5, pp.2578~2583 (2011)
  24. Khan MA, "Halo-tolerant rhizospheric Arthrobacter woluwensis AK1mitigates salt stress and induces physio-hormonal changes and expression of GmST1 and GmLAX3 in soybean", Symbiosis, 77, pp.9~21 (2019)
  25. Khan MA, "Rhizobacteria AK1 remediates the toxic effects of salinity stress via regulation of endogenous phytohormones and gene expression in soybean", Biochem. J, 476, pp.2393~2409 (2019)
  26. Mun B-G, "Streptomyces sp. LH 4 promotes plant growth and resistance against Sclerotinia sclerotiorum in cucumber via modulation of enzymatic and defense pathways", Plant Soil, 448, pp.87~103 (2020)
  27. Freeman D, "New method for detecting slime production by coagulase negative staphylococci", J. Clin. Pathol, 42, pp.872~874 (1989)
  28. Khan MA, "Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1", Ann. Microbiol., 69, pp.797~808 (2019)
  29. Kang S-M, "Enterobacter sp. SE992-induced regulation of amino acids, sugars, and hormones in cucumber plants improves salt tolerance", Acta Physiol. Plant., 37, pp.149~ (2015)
  30. Park Y-G, "Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones", PLoS One, 12, pp.e0173203~ (2017)
  31. Khan MA, "Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application", PLoS One, 15, pp.e0232228~ (2020)
  32. Khan MA, "Gibberellin application ameliorates the adverse impact of short-term flooding on Glycine max L", Biochem. J, 475, pp.2893~2905 (2018)
  33. Khan MA, "Exogenous application of nitric oxide donors regulates shortterm flooding stress in soybean", PeerJ, 7, pp.e7741~ (2019)
  34. Jan R, "Metal resistant endophytic bacteria reduces cadmium, nickel toxicity, and enhances expression of metal stress related genes with improved growth of Oryza sativa, via regulating its antioxidant machinery and endogenous hormones", Plants(Basel), 8, pp.363~ (2019)
  35. Frahry G, "NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay", Planta, 212, pp.175~183 (2001)
  36. Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
  37. Chaoui A, "Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.)", Plant Sci., 127, pp.139~147 (1997)
  38. Gowrishankar S, "Modulation of Staphylococcus epidermidis(RP62A)extracellular polymeric layer by marine cyclic dipeptide-cyclo(l-leucyl-l-prolyl)thwarts biofilm formation", Biochim. Biophys. Acta Biomembr, 1859, pp.1254~1262 (2017)
  39. Russell DW, "Molecular cloning: a laboratory manual Vol. 1", pp.112~ (2001), Cold Spring Harbor Laboratory
  40. Tamura K, "MEGA6: Molecular evolutionary genetics analysis version 6.0", Mol. Biol. Evol., 30, pp.2725~2729 (2013)
  41. Yandigeri MS, "Drought-tolerant endophytic actinobacteria promote growth of wheat(Triticum aestivum)under water stress conditions", Plant Growth Regul, 68, pp.411~420 (2012)
  42. Marasco R, "A drought resistance-promoting microbiome is selected by root system under desert farming", PLoS One, 7, pp.e48479~e48479 (2012)
  43. 임종희, "Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus licheniformis K11 in Pepper", The Plant Pathology Journal, 29(2), pp.201~208 (2013), 한국식물병리학회[DOI]
  44. Mayak S, "Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers", Plant Sci, 166, pp.525~530 (2004)
  45. Zhang W, "Growth-promoting bacteria alleviates drought stress of G. uralensis through improving photosynthesis characteristics and water status", J. Plant Interact., 14, pp.580~589 (2019)
  46. Cho SM, "2R, 3R-butanediol, a bacterial volatile produced by Pseudomonas chlororaphis O6, is involved in induction of systemic tolerance to drought in Arabidopsis thaliana", Mol. Plant Microbe. Interact, 21, pp.1067~1075 (2008)
  47. Gagné-Bourque F, "Alleviation of drought stress and metabolic changes in timothy (Phleum pratense L.) colonized with Bacillus subtilis B26", Front. Plant Sci., 7, pp.584~ (2016)
  48. Casanovas EM, "Azospirillum inoculation mitigates water stress effects in maize seedlings", Cereal Res. Commun., pp.343~350 (2002)
  49. Kiani S, "Effect of mannitol stress on morphological, biochemical and polyphenol parameters in broccoli sprouts (Brassica oleracea var. Italica)", Appl. Ecol. Environ. Res., 16, pp.2043~2058 (2018)
  50. Beacham AM, "Analysis of Brassica oleracea early stage abiotic stress responses reveals tolerance in multiple crop types and for multiple sources of stress", J. Sci. Food Agric, 97, pp.5271~5277 (2017)
  51. Rahbarian R, "Drought stress effects on photosynthesis, chlorophyll fluorescence and water relations in tolerant and susceptible chickpea (Cicer arietinum L.) genotypes", Acta Biol. Crac. Ser. Bot., 53, pp.47~56 (2011)
  52. Wu H, "Physiological evaluation of drought stress tolerance and recovery in cauliflower (Brassica oleracea L.) seedlings treated with methyl jasmonate and coronatine", J. Plant Growth Regul., 31, pp.113~123 (2012)
  53. Vurukonda SSKP, "Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria", Microbiol. Res, 184, pp.13~24 (2016)
  54. Selvakumar G, "Bacteria in agrobiology: Stress management", pp.205~224 (2012), Springer
  55. Wang Z, "Effects of drought stress on photosynthesis and photosynthetic electron transport chain in young apple tree leaves", Biol. Open., 7, pp.bio035279~ (2018)
  56. Li J, "Influence of drought stress on photosynthetic characteristics and protective enzymes of potato at seedling stage", J. Saudi Soc. Agric. Sci, 16, pp.82~88 (2017)
  57. Heidari M, "Effects of water stress and inoculation with plant growth promoting rhizobacteria (PGPR) on antioxidant status and photosynthetic pigments in basil (Ocimum basilicum L.)", J. Saudi Soc. Agric. Sci., 11, pp.57~61 (2012)
  58. Vardharajula S, "Drought-tolerant plant growth promoting Bacillus spp.: effect on growth, osmolytes, and antioxidant status of maize under drought stress", J. Plant Interact., 6, pp.1~14 (2011)
  59. Gusain YS, "Bacterial mediated amelioration of drought stress in drought tolerant and susceptible cultivars of rice (Oryza sativa L.)", Afr. J. Biotechnol., 14, pp.764~773 (2015)
  60. de Ollas C, "Physiological impacts of ABA–JA interactions under water-limitation", Plant Mol. Biol, 91, pp.641~650 (2016)
  61. Verma V, "Plant hormone-mediated regulation of stress responses", BMC Plant Biol, 16, pp.86~86 (2016)
  62. Tiwari S, "Bacillus amyloliquefaciens confers tolerance to various abiotic stresses and modulates plant response to phytohormones through osmoprotection and gene expression regulation in rice", Front. Plant Sci, 8, pp.1510~ (2017)
  63. Curá JA, "Inoculation with Azospirillum sp. and Herbaspirillum sp. bacteria increases the tolerance of maize to drought stress", Microorganisms, 5, pp.41~ (2017)
  64. 구영모, "Salicylic Acid as a Safe Plant Protector and Growth Regulator", The Plant Pathology Journal, 36(1), pp.1~10 (2020), 한국식물병리학회[DOI]
  65. Wang Q-J, "The enhancement of tolerance to salt and cold stresses by modifying the redox state and salicylic acid content via the cytosolic malate dehydrogenase gene in transgenic apple plants", Plant Biotechnol. J, 14, pp.1986~1997 (2016)
  66. Yang Y, "Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress", Plant J, 40, pp.909~919 (2004)
  67. Wang LJ, "Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves", BMC Plant Biol, 10, pp.34~ (2010)
  68. Lopez-Delgado H, "Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2", J. Exp. Bot, 49, pp.713~720 (1998)
  69. Senaratna T, "Acetyl salicylic acid(Aspirin)and salicylic acid induce multiple stress tolerance in bean and tomato plants", Plant Growth Regul, 30, pp.157~161 (2000)
  70. Clarke SM, "Salicylic acid dependent signaling promotes basal thermotolerance but is not essential for acquired thermotolerance in Arabidopsis thaliana", Plant J, 38, pp.432~447 (2004)
  71. Wang L-J, "Salicylic acid-induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant systems in young grape plants", Plant Sci, 170, pp.685~694 (2006)
  72. Shahzad R, "Seed-borne endophytic Bacillus amyloliquefaciens RWL-1produces gibberellins and regulates endogenous phytohormones of Oryza sativa", Plant Physiol. Biochem, 106, pp.236~243 (2016)

이 간행물의 다른 논문 (8건)