Transcription Analysis of Recombinant Trichoderma reesei HJ-48 to Compare the Molecular Basis for Fermentation of Glucose and Xylose
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Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.
Abstract
Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.
참고문헌 (54)
- Jianning Zheng, "Comparison of Bioethanol Production by Candida molischiana and Saccharomyces cerevisiae from Glucose, Cellobiose, and Cellulose", Journal of Microbiology and Biotechnology, 29(6), pp.905~912 (2019), 한국미생물·생명공학회[DOI]
- Kumar V, "Bioconversion of pentose sugars to value added chemicals and fuels : Recent trends, challenges and possibilities", Bioresour. Technol, 269, pp.443~451 (2018)
- Hongting Tang, "High β-Glucosidase Secretion in Saccharomyces cerevisiae Improves the Efficiency of Cellulase Hydrolysis and Ethanol Production in Simultaneous Saccharification and Fermentation", Journal of Microbiology and Biotechnology, 23(11), pp.1577~1585 (2013), 한국미생물·생명공학회[DOI]
- Nijland JG, "Improving pentose fermentation by preventing ubiquitination of hexose transporters in Saccharomyces cerevisiae", Biotechnol. Biofuels, 9, pp.158~ (2016)
- Liu H, "Engineering microbes for direct fermentation of cellulose to bioethanol", Crit. Rev. Biotechnol, 38, pp.1089~1105 (2018)
- Zongsheng Zou, "Efficient Isolation and Characterization of a Cellulase Hyperproducing Mutant Strain of Trichoderma reesei", Journal of Microbiology and Biotechnology, 28(9), pp.1473~1481 (2018), 한국미생물·생명공학회
- Xu Q, "Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocellulose", Curr. Opin. Biotechnol, 20, pp.364~371 (2009)
- Li YH, "Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover", Biotechnol. Biofuels, 11, pp.49~ (2018)
- Huang J, "Direct ethanol production from lignocellulosic sugars and sugarcane bagasse by a recombinant Trichoderma reesei strain HJ48", ScientificWorldJournal, 2014, pp.798683~ (2014)
- Walker ME, "Genome-wide identification of the Fermentome;genes required for successful and timely completion of wine-like fermentation by Saccharomyces cerevisiae", BMC Genomics, 15, pp.552~ (2014)
- Matsushika A, "Transcription analysis of recombinant industrial and laboratory Saccharomyces cerevisiae strains reveals the molecular basis for fermentation of glucose and xylose", Microb. Cell Fact, 13, pp.16~ (2014)
- Jun Huang, "Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq", Journal of Microbiology and Biotechnology, 26(7), pp.1242~1251 (2016), 한국미생물·생명공학회[DOI]
- Yang M, "RNA-Seq uncovers SNPs and alternative splicing events in asian lotus(Nelumbo nucifera)", PLoS One, 10, pp.e0125702~ (2015)
- Martinez D, "Genome sequencing and analysis of the biomassdegrading fungus Trichoderma reesei (syn. Hypocrea jecorina)", Nat. Biotechnol., 26, pp.553~560 (2008)
- Kim D, "TopHat2 : accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions", Genome Biol, 14, pp.R36~ (2013)
- Anders S, "HTSeq-a Python framework to work with high-throughput sequencing data", Bioinformatics, 31, pp.166~169 (2015)
- Mortazavi A, "Mapping and quantifying mammalian transcriptomes by RNA-Seq", Nat. Methods, 5, pp.621~628 (2008)
- Wang L, "DEGseq : an R package for identifying differentially expressed genes from RNAseq data", Bioinformatics, 26, pp.136~138 (2010)
- Benjamini Y, "Controlling the false discovery rate : a practical and powerful approach to multiple testing", J. R. Statist. Soc. B, 1, pp.289~300 (1995)
- Anders S, "Differential expression analysis for sequence count data", Genome Biol, 11, pp.R106~ (2010)
- Mao XZ, "Automated genome annotation and pathway identification using the KEGG Orthology(KO)as a controlled vocabulary", Bioinformatics, 21, pp.3787~3793 (2005)
- Li J, "Transcriptional comparison of the filamentous fungus Neurospora crassa growing on three major monosaccharides D-glucose, D-xylose and L-arabinose", Biotechnol. Biofuels, 7, pp.31~ (2014)
- Stincone A, "The return of metabolism : biochemistry and physiology of the pentose phosphate pathway", Biol. Rev. Camb. Philos. Soc, 90, pp.927~963 (2015)
- Matsushika A, "Transcription analysis of recombinant industrial and laboratory Saccharomyces cerevisiae strains reveals the molecular basis for fermentation of glucose and xylose", Microb. Cell Fact, 13, pp.16~ (2014)
- Runquist D, "Increased expression of the oxidative pentose phosphate pathway and gluconeogenesis in anaerobically growing xylose-utilizing Saccharomyces cerevisiae", Microb. Cell Fact, 8, pp.49~ (2009)
- Rodriguez A, "The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae", Biochem. J, 355, pp.625~631 (2001)
- Brown SR, "Design of experiments methodology to build a multifactorial statistical model describing the metabolic interactions of alcohol dehydrogenase isozymes in the ethanol biosynthetic pathway of the yeast Saccharomyces cerevisiae", Acs Synth. Biol, 7, pp.1676~1684 (2018)
- Yao YX, "Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis", J. Plant Physiol, 168, pp.474~480 (2011)
- Zeng WY, "Transcriptomes of a xylose-utilizing industrial flocculating Saccharomyces cerevisiae strain cultured in media containing different sugar sources", AMB Express, 6, pp.51~ (2016)
- Hossain S, "Overview of catalytic properties of fungal xylose reductases and molecular engineering approaches for improved xylose utilisation in yeast", Appl. Food Biotechnol, 5, pp.47~58 (2018)
- Li HB, "Enabling glucose", xylose co-transport in yeast through the directed evolution of a sugar transporter, 2016, pp.100, 10215~10223
- Moyses DN, "Xylose fermentation by Saccharomyces cerevisiae : challenges and prospects", Int. J. Mol. Sci, 17, pp.207~ (2016)
- Yang BX, "The effect of xylose reductase genes on xylitol production by industrial Saccharomyces cerevisiae in fermentation of glucose and xylose", Process Biochem, 95, pp.122~130 (2020)
- Hong Y, "Overexpression of D-xylose reductase(xyl1)gene and antisense inhibition of D-xylulokinase(xyiH)gene increase xylitol production in Trichoderma reesei", Biomed. Res. Int, 2014, pp.169705~ (2014)
- Perl A, "Oxidative stress, inflammation and carcinogenesis are controlled through the pentose phosphate pathway by transaldolase", Trends Mol. Med, 17, pp.395~403 (2011)
- Souto-Maior AM, "Crabtree-negative characteristics of recombinant xylose-utilizing Saccharomyces cerevisiae", J. Biotechnol, 143, pp.119~123 (2009)
- Kobayashi Y, "Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production", J. Ind. Microbiol. Biotechnol, 44, pp.879~891 (2017)
- Feng Q, "Signature pathway expression of xylose utilization in the genetically engineered industrial yeast Saccharomyces cerevisiae", PLoS One, 13, pp.e0195633~ (2018)
- Chomvong K, "Bypassing the pentose phosphate pathway : towards modular utilization of xylose", PLoS One, 11, pp.e0158111~ (2016)
- Kurylenko OO, "Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea(Hansenula)polymorpha", Biotechnol. Biofuels, 11, pp.197~ (2018)
- Jeppsson M, "Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose", Appl. Environ. Microbiol, 68, pp.1604~1609 (2002)
- Miskovic L, "A design-build-test cycle using modeling and experiments reveals interdependencies between upper glycolysis and xylose uptake in recombinant S. cerevisiae and improves predictive capabilities of large-scale kinetic models", Biotechnol. Biofuels, 10, pp.166~ (2017)
- Matsushika A, "Fermentation of xylose causes inefficient metabolic state due to carbon", energy starvation and reduced glycolytic flux in recombinant industrial Saccharomyces cerevisiae, 2013, pp.8, e69005~
- Hortschansky P, "Interaction of HapX with the CCAATbinding complex—a novel mechanism of gene regulation by iron", EMBO J, 26, pp.3157~3168 (2007)
- Zeilinger S, "The Hypocrea jecorina HAP 2", 3, Mol. Genet. Genomics, pp.2001, 266[DOI]
- Buschlen S, "The S. cerevisiae HAP complex, a key regulator of mitochondrial function, coordinates nuclear and mitochondrial gene expression", Comp. Funct. Genomics, 4, pp.37~46 (2003)
- Kato M, "An overview of the CCAAT-Box binding factor in filamentous fungi : assembly, nuclear translocation, and transcriptional enhancement", Biosci. Biotechnol. Biochem, 69, pp.663~672 (2014)
- Young EM, "Rewiring yeast sugar transporter preference through modifying a conserved protein motif", Proc. Natl. Acad. Sci. USA, 111, pp.131~136 (2014)
- Colabardini AC, "Functional characterization of a xylose transporter in Aspergillus nidulans", Biotechnol. Biofuels, 7, pp.46~ (2014)
- Ozcan S, "Function and regulation of yeast hexose transporters", Microbiol. Mol. Biol. Rev, 63, pp.554~569 (1999)
- MH Saier Jr, "The major facilitator superfamily", J. Mol. Microbiol. Biotechnol, 1, pp.257~279 (1999)
- Sharma NK, "Xylose transport in yeast for lignocellulosic ethanol production : current status", J. Biosci. Bioeng, 125, pp.259~267 (2018)
- Du J, "Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis", Mol. Biosyst, 6, pp.2150~2156 (2010)
- Sloothaak J, "Identification and functional characterization of novel xylose transporters from the cell factories Aspergillus niger and Trichoderma reesei", Biotechnol. Biofuels, 9, pp.148~ (2016)
