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Transcription Analysis of Recombinant Trichoderma reesei HJ-48 to Compare the Molecular Basis for Fermentation of Glucose and Xylose

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초록

Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.

Abstract

Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.

참고문헌 (54)

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관련 논문AI추천

Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-SeqDong Chen, Jun Huang, Qingyan Wang, Renzhi Wu, Ribo Huang한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제7호Comprehensive Characterization of Mutant Pichia stipitis Co-Fermenting Cellobiose and Xylose through Genomic and Transcriptomic Analyses김수정, 이원흥, Choi Hyo-Jin, Kim Dae-Hwan, Lee Sangmin, Lee Yu Rim한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제11호Efficient Isolation and Characterization of a Cellulase Hyperproducing Mutant Strain of Trichoderma reeseiAiyong He, Jiaxing Xu, Tingzhou Zhang, Yu Deng, Yunying Zhao, Zongsheng Zou한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제9호Comparison of Bioethanol Production by Candida molischiana and Saccharomyces cerevisiae from Glucose, Cellobiose, and Cellulose김범수, Abhishek Negi, Chanin Khomlaem, Jianning Zheng한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제6호Xylitol Production by Kluyveromyces marxianus 36907-FMEL1 at High Temperature was Considerably Increased through the Optimization of Agitation Conditions권덕호, 김진성, 박재범, 신원철, 장승원, 하석진, 홍억기한국미생물·생명공학회 · 한국미생물·생명공학회지2017 · 제45권 제1호Effects of Engineered Saccharomyces cerevisiae Fermenting Cellobiose through Low-Energy-Consuming Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation이원흥, Choi Hyo-Jin, Jin Yong-Su한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제1호

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