Antioxidant and Antimelanogenic Activities of Kimchi-Derived Limosilactobacillus fermentum JNU532 in B16F10 Melanoma Cells
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Melanin is a natural skin pigment produced by specialized cells called melanocytes via a multistage biochemical pathway known as melanogenesis, involving the oxidation and polymerization of tyrosine. Melanogenesis is initiated upon exposure to ultraviolet (UV) radiation, causing the skin to darken, which protects skin cells from UVB radiation damage. However, the abnormal accumulation of melanin may lead to the development of certain skin diseases, including skin cancer. In this study, the antioxidant and antimelanogenic activities of the cell-free supernatant (CFS) of twenty strains were evaluated. Based on the results of 60% 2,2-diphenyl-1-picrylhydrazyl scavenging activity, 21% 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) scavenging capacity, and a 50% ascorbic acid equivalent ferric reducing antioxidant power value, Limosilactobacillus fermentum JNU532 was selected as the strain with the highest antioxidant potential. No cytotoxicity was observed in cells treated with the CFS of L. fermentum JNU532. Tyrosinase activity was reduced by 16.7% in CFStreated B16F10 cells (but not in the cell-free system), with >23.2% reduction in melanin content upon treatment with the L. fermentum JNU532-derived CFS. The inhibitory effect of the L. fermentum JNU532-derived CFS on B16F10 cell melanogenesis pathways was investigated using quantitative reverse transcription polymerase chain reaction and western blotting. The inhibitory effects of the L. fermentum JNU532-derived CFS were mediated by inhibiting the transcription of TYR, TRP-1, TRP-2, and MITF and the protein expression of TYR, TRP-1, TRP-2, and MITF. Therefore, L. fermentum JNU532 may be considered a potentially useful, natural depigmentation agent.
Abstract
Melanin is a natural skin pigment produced by specialized cells called melanocytes via a multistage biochemical pathway known as melanogenesis, involving the oxidation and polymerization of tyrosine. Melanogenesis is initiated upon exposure to ultraviolet (UV) radiation, causing the skin to darken, which protects skin cells from UVB radiation damage. However, the abnormal accumulation of melanin may lead to the development of certain skin diseases, including skin cancer. In this study, the antioxidant and antimelanogenic activities of the cell-free supernatant (CFS) of twenty strains were evaluated. Based on the results of 60% 2,2-diphenyl-1-picrylhydrazyl scavenging activity, 21% 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) scavenging capacity, and a 50% ascorbic acid equivalent ferric reducing antioxidant power value, Limosilactobacillus fermentum JNU532 was selected as the strain with the highest antioxidant potential. No cytotoxicity was observed in cells treated with the CFS of L. fermentum JNU532. Tyrosinase activity was reduced by 16.7% in CFStreated B16F10 cells (but not in the cell-free system), with >23.2% reduction in melanin content upon treatment with the L. fermentum JNU532-derived CFS. The inhibitory effect of the L. fermentum JNU532-derived CFS on B16F10 cell melanogenesis pathways was investigated using quantitative reverse transcription polymerase chain reaction and western blotting. The inhibitory effects of the L. fermentum JNU532-derived CFS were mediated by inhibiting the transcription of TYR, TRP-1, TRP-2, and MITF and the protein expression of TYR, TRP-1, TRP-2, and MITF. Therefore, L. fermentum JNU532 may be considered a potentially useful, natural depigmentation agent.
참고문헌 (29)
- Cherie JZ, "An overview of probiotics, prebiotics and synbiotics in the functional food concept : perspectives and future strategies", Int. Dairy J, 8, pp.473~479 (1998)
- Zheng J, "A taxonomic note on the genus Lactobacillus : Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae", Int. J. Syst. Evol. Microbiol, 70, pp.2782~2858 (2020)
- Parvez S, "Probiotics and their fermented food products are beneficial for health", J. Appl. Microbiol, 100, pp.1171~1185 (2006)
- Gilliland SE, "Importance of bile tolerance of Lactobacillus Acidophilus used as a dietary adjunct", J. Dairy Sci, 67, pp.3045~3051 (1984)
- Paulina M, "Effects of probiotics, prebiotics, and synbiotics on human health", Nutrients, 9, pp.1021~ (2017)
- 김태락, "Anti-inflammatory effects of Lactobacillus reuteri LM1071 via MAP kinase pathway in IL-1β-induced HT-29 cells", 한국축산학회지, 62(6), pp.864~874 (2020), 한국축산학회[DOI]
- Virtanen T, "Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria", J. Appl. Microbiol, 102, pp.106~115 (2007)
- Divya S, "Anti-aging effects of probiotics", J. Drugs Dermatol, 15, pp.9~12 (2016)
- 김혜림, "Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells", Molecules and Cells, 38(2), pp.163~170 (2015), 한국분자세포생물학회[DOI]
- F. Wöhler, "Lehrbuch der Chemie, Vol. 9", ~ (1840)
- Solano F, "Melanins: Skin pigments and much more-types, structural models, biological functions, and formation routes", New J. Sci, 28 (2014)[DOI]
- Schallreuter KU, "Regulation of melanogenesis controversies and new concepts", Exp. Dermatol, 17, pp.395~404 (2007)
- Chang TS, "An updated review of tyrosinase inhibitors", Int. J. Mol. Sci, 10, pp.2440~2475 (2009)
- Bellei B, "p38 regulates pigmentation via proteasomal degradation of tyrosinase", J. Biol. Chem, 285, pp.7288~7299 (2010)
- Baek SH, "Sesamol decreases melanin biosynthesis in melanocyte cells and zebrafish : possible involvement of MITF via the intracellular cAMP and p38", JNK signaling pathways, 2015, pp.24, 761~766
- Chan CF, "Fermented broth in tyrosinase and melanogenesis inhibition", Molecules, 19, pp.13122~13135 (2014)
- Vance KW, "The transcription network regulating melanocyte development and melanoma", Pigment Cell Res, 17, pp.318~325 (2004)
- Yoshinori M, "Regulation of human skin pigmentation and responses to ultraviolet radiation", J. Invest. Dermatol, 130, pp.1685~1696 (2010)
- Beani JC, "Ultraviolet A-induced DNA damage : role in skin cancer", Bull. Acad. Natl. Med, 198, pp.273~295 (2014)
- Pillaiyar T, "Skin whitening agents : medicinal chemistry perspective of tyrosinase inhibitors", J. Enzyme Inhib. Med. Chem, 32, pp.403~425 (2017)
- Blois MS, "Free radicals in biological systems", Science, 132, pp.306~307 (1960)
- Fitzpatrick TB, "Albinism : some thoughts on the color problem and integration of dermatology and medicine", J. Invest. Dermatol, 35, pp.209~214 (1960)
- Zhang CJ, "Lactobacillus Plantarum CGMCC8198-fermented milk inhibits melanogenesis in B16F10 melanoma cells", Food Sci. Technol. Res, 23, pp.669~678 (2017)
- Md BA, "Inhibition of melanogenesis by jineol from Scolopendra subspinipes mutilans via MAP-Kinase mediated MITF downregulation and the proteasomal degradation of tyrosinase", Sci. Rep, 7, pp.45858~ (2017)
- Sang JA, "Regulation of melanin synthesis by selenium containing carbohydrates", Chem. Pharm. Bull, 54, pp.281~286 (2006)
- Chung SY, "Fermented rice bran downregulates MITF expression and leads to inhibition of alpha-MSH-induced melanogenesis in B16F10 melanoma", Biosci. Biotechnol. Biochem, 73, pp.1704~1710 (2009)
- Lee HJ, "Functional properties of Lactobacillus strains isolated from kimchi", Int. J. Food Microbiol, 145, pp.155~161 (2011)
- Tanja L, "Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces", Anaerobe, 16, pp.293~300 (2009)
- Rocío M, "Human milk is a source of lactic acid bacteria for the infant gut", J. Pediatr, 143, pp.754~758 (2003)