Supercooling Pretreatment Improves the Shelf-Life of Freeze-Dried Leuconostoc mesenteroides WiKim32
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at −5o C on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPShydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at −5o C. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.
Abstract
Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at −5o C on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPShydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at −5o C. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.
참고문헌 (22)
- Bintsis T, "Lactic acid bacteria : their applications in foods", J. Bacteriol. Mycol, 6, pp.89~94 (2018)
- Chang JY, "Improvements in the quality and shelf life of kimchi by fermentation with the induced bacteriocinproducing strain, Leuconostoc citreum GJ7 as a starter", J. Food Sci, 75, pp.103~110 (2010)
- Jeong SH, "Effects of red pepper powder on microbial communities and metabolites during kimchi fermentation", Int. J. Food Microbiol, 160, pp.252~259 (2013)
- 이모은, "Starter Cultures for Kimchi Fermentation", Journal of Microbiology and Biotechnology, 25(5), pp.559~568 (2015), 한국미생물·생명공학회[DOI]
- Martín MJ, "Microencapsulation of bacteria : a review of different technologies and their impact on the probiotic effects", Innov. Food Sci. Emerg. Technol, 27, pp.15~25 (2015)
- Thomas Heidebach, "Influence of casein-based microencapsulation on freeze-drying and storage of probiotic cells", Journal of Food Engineering, 98(3), pp.309~316 (2010), Elsevier BV
- Patist A, "Preservation mechanisms of trehalose in food and biosystems", Colloids and Surfaces B : Biointerfaces, 40, pp.107~113 (2005)
- 곽현정, "Protective Effect of Soy Powder and Microencapsulation on Freeze-dried Lactobacillus brevis WK12 and Lactococcus lactis WK11 during Storage", Food Science and Biotechnology, 24(6), pp.2155~2160 (2015), 한국식품과학회[DOI]
- Haffner FB, "Encapsulation of probiotics : Insights into academic and industrial approaches", AIMS Mater. Sci, 3, pp.114~136 (2016)
- Fernández Murga ML, "Influence of growth temperature on cryotolerance and lipid composition of Lactobacillus acidophilus", J. Appl. Microbiol, 88, pp.342~348 (2000)
- Sooyeon Song, "Cold stress improves the ability of Lactobacillus plantarum L67 to survive freezing", International Journal of Food Microbiology, 191, pp.135~143 (2014), Elsevier BV[DOI]
- Choi IS., "Effect of cation influx on the viability of freeze-dried Lactobacillus brevis WiKim0069", Appl. Sci. -Basel, 8, pp.2189~ (2018)
- Choi IS, "Shelf-life extension of freeze-dried Lactobacillus brevis WiKim0069 using supercooling pretreatment", Lwt-Food Sci. Technol, 112, pp.108230~ (2019)
- Lee M, "DNAzyme-based quantitative loop-mediated isothermal amplification for strainspecific detection of starter kimchi fermented with Leuconostoc mesenteroides WiKim32", Food Chem, 333, pp.127343~ (2020)
- Choi IS, "Production, characterization, and antioxidant activities of an exopolysaccharide extracted from spent media wastewater after Leuconostoc mesenteroides WiKim32 fermentation", ACS Omega, 6, pp.8171~8178 (2021)
- De Vuyst L, "Heteropolysaccharides from lactic acid bacteria", FEMS Microbiol. Rev, 23, pp.153~177 (1999)
- Nguyen PT, "Exopolysaccharide production by lactic acid bacteria : the manipulation of environmental stresses for industrial applications", AIMS Microbiol, 6, pp.451~469 (2020)
- Fuchs BB, "Our paths might cross : the role of the fungal cell wall integrity pathway in stress response and cross talk with other stress response pathways", Eukaryot. Cell, 8, pp.1616~1625 (2009)
- Claret S, "The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae", Eukaryot. Cell, 4, pp.1375~1386 (2005)
- Prost LR, "The Salmonellae PhoQ sensor : mechanisms of detection of phagosome signals", Cell. Microbiol, 10, pp.576~582 (2008)
- Dalebroux ZD, "Salmonellae PhoPQ regulation of the outer membrane to resist innate immunity", Curr. Opin. Microbiol, 17, pp.106~113 (2014)
- Kim SJ, "Cryoprotective properties and preliminary characterization of exopolysaccharide(PArcPo 15)produced by the arctic bacterium Pseudoalteromonas elyakovii ArcPo 15", Prep. Biochem. Biotechnol, 46, pp.261~266 (2016)