Characterization of exopolysaccharide-producing lactic acid bacteria from Taiwanese ropy fermented milk and their application in low-fat fermented milk
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Objective: The aim of this study was to characterize the exopolysaccharides (EPS)-producing lactic acid bacteria from Taiwanese ropy fermented milk (TRFM) for developing a clean label low-fat fermented milk. Methods: Potential isolates from TRFM were selected based on the Gram staining test and observation of turbid suspension in the culture broth. Random amplified polymorphic DNA-polymerase chain reaction, 16S rRNA gene sequencing, and API CHL 50 test were used for strain identification. After evaluation of EPS concentration, target strains were introduced to low-fat milk fermentation for 24 h. Fermentation characters were checked: pH value, acidity, viable count, syneresis, and viscosity. Sensory evaluation of fermented products was carried out by 30 volunteers, while the storage test was performed for 21 days at 4°C. Results: Two EPS-producing strains (APL15 and APL16) were isolated from TRFM and identified as Lactococcus (Lc.) lactis subsp. cremoris. Their EPS concentrations in glucose and lactose media were higher than other published strains of Lc. lactis subsp. cremoris. Low-fat fermented milk separately prepared with APL15 and APL16 reached pH 4.3 and acidity 0.8% with a viable count of 9 log colony-forming units/mL. The physical properties of both products were superior to the control yogurt, showing significant improvements in syneresis and viscosity (p<0.05). Our low-fat products had appropriate sensory scores in appearance and texture according to sensory evaluation. Although decreasing viable cells of strains during the 21-day storage test, low-fat fermented milk made by APL15 exhibited stable physicochemical properties, including pH value, acidity, syneresis and sufficient viable cells throughout the storage period. Conclusion: This study demonstrated that Lc. lactis subsp. cremoris APL15 isolated from TRFM had good fermentation abilities to produce low-fat fermented milk. These data indicate that EPS-producing lactic acid bacteria have great potential to act as natural food stabilizers for low-fat fermented milk.
Abstract
Objective: The aim of this study was to characterize the exopolysaccharides (EPS)-producing lactic acid bacteria from Taiwanese ropy fermented milk (TRFM) for developing a clean label low-fat fermented milk. Methods: Potential isolates from TRFM were selected based on the Gram staining test and observation of turbid suspension in the culture broth. Random amplified polymorphic DNA-polymerase chain reaction, 16S rRNA gene sequencing, and API CHL 50 test were used for strain identification. After evaluation of EPS concentration, target strains were introduced to low-fat milk fermentation for 24 h. Fermentation characters were checked: pH value, acidity, viable count, syneresis, and viscosity. Sensory evaluation of fermented products was carried out by 30 volunteers, while the storage test was performed for 21 days at 4°C. Results: Two EPS-producing strains (APL15 and APL16) were isolated from TRFM and identified as Lactococcus (Lc.) lactis subsp. cremoris. Their EPS concentrations in glucose and lactose media were higher than other published strains of Lc. lactis subsp. cremoris. Low-fat fermented milk separately prepared with APL15 and APL16 reached pH 4.3 and acidity 0.8% with a viable count of 9 log colony-forming units/mL. The physical properties of both products were superior to the control yogurt, showing significant improvements in syneresis and viscosity (p<0.05). Our low-fat products had appropriate sensory scores in appearance and texture according to sensory evaluation. Although decreasing viable cells of strains during the 21-day storage test, low-fat fermented milk made by APL15 exhibited stable physicochemical properties, including pH value, acidity, syneresis and sufficient viable cells throughout the storage period. Conclusion: This study demonstrated that Lc. lactis subsp. cremoris APL15 isolated from TRFM had good fermentation abilities to produce low-fat fermented milk. These data indicate that EPS-producing lactic acid bacteria have great potential to act as natural food stabilizers for low-fat fermented milk.
참고문헌 (30)
- Sandrou DK, "Low-fat", calorie foods : current state and perspectives, 2000, pp.40, 427~447
- Babio N, "Consumption of yogurt, low-fat milk, and other low-fat dairy products is associated with lower risk of metabolic syndrome incidence in an elderly Mediterranean population", J Nutr, 145, pp.2308~2316 (2015)[DOI]
- Forss DA, "Role of lipids in flavors", J Agric Food Chem, 17, pp.681~685 (1969)[DOI]
- Brauss MS, "Altering the fat content affects flavor release in a model yogurt system", J Agric Food Chem, 47, pp.2055~2059 (1999)[DOI]
- Prasanna PHP, "Screening human intestinal Bifidobacterium strains for growth, acidification, EPS production and viscosity potential in lowfat milk", Int Dairy J, 23, pp.36~44 (2012)[DOI]
- Behare PV, "Exopolysaccharides producing Lactobacillus fermentum strain for enhancing rheological and sensory attributes of low-fat dahi", J Food Sci Technol, 50, pp.1228~1232 (2013)[DOI]
- London LE, "Use of Lactobacillus mucosae DPC 6426, an exopolysaccharide-producing strain, positively influences the techno-functional properties of yoghurt", Int Dairy J, 40, pp.33~38 (2015)[DOI]
- Prasanna PHP, "Microbiological, chemical and rheological properties of low fat set yoghurt produced with exopolysaccharide(EPS)producing Bifidobacterium strains", Food Res Int, 51, pp.15~22 (2013)[DOI]
- Wang SY, "Identification of lactic acid bacteria in Taiwanese ropy fermented milk and evaluation of their microbial ecology in bovine and caprine milk", J Dairy Sci, 94, pp.623~635 (2011)[DOI]
- Caggianiello G, "Exopolysaccharides produced by lactic acid bacteria : from health-promoting benefits to stress tolerance mechanisms", Appl Microbiol Biotechnol, 100, pp.3877~3886 (2016)[DOI]
- Watanabe K, "Diversity of lactic acid bacteria and yeasts in Airag and Tarag, traditional fermented milk products of Mongolia", World J Microbiol Biotechnol, 24, pp.1313~1325 (2008)[DOI]
- Kumar S, "MEGA7 : Molecular evolutionary genetics analysis version 7. 0 for bigger datasets", Mol Biol Evol, 33, pp.1870~1874 (2016)[DOI]
- Dubois M, "Colorimetric method for determination of sugars and related substances", Anal Chem, 28, pp.350~356 (1956)[DOI]
- "Dried milk: determination of titratable acidity (ISO 6901:2010)" (2010)[URL]
- Mani-Lopez E, "Probiotic viability and storage stability of yogurts and fermented milks prepared with several mixtures of lactic acid bacteria", J Dairy Sci, 97, pp.2578~2590 (2014)[DOI]
- Damin MR, "Effect of cold storage on culture viability and some rheological properties of fermented milk prepared with yogurt and probiotic bacteria", J Texture Stud, 39, pp.40~55 (2008)[DOI]
- Rimada PS, "Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey", Lait, 83, pp.79~87 (2003)[DOI]
- Meilgaard MC, "Sensory evaluation techniques" (1999), CRC Press
- Tanigawa K, "Identification and typing of Lactococcus lactis by matrix-assisted laser desorption ionization-time of flight mass spectrometry", Appl Environ Microbiol, 76, pp.4055~4062 (2010)[DOI]
- Perez T, "Lactococcus lactis subsp. tructae subsp. nov. isolated from the intestinal mucus of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss)", Int J Syst Evol Microbiol, 61, pp.1894~1898 (2011)[DOI]
- Meucci A, "Lactococcus hircilactis sp. nov. and Lactococcus laudensis sp. nov., isolated from milk", Int J Syst Evol Microbiol, 65, pp.2091~2096 (2015)[DOI]
- Li TT, "Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov", Int J Syst Evol Microbiol, 71, pp.004727~ (2021)[DOI]
- Boels IC, "Engineering of carbon distribution between glycolysis and sugar nucleotide biosynthesis in Lactococcus lactis", Appl Environ Microbiol, 69, pp.1129~1136 (2003)[DOI]
- Marshall VM, "Analysis and production of two exopolysaccharides from Lactococcus lactis subsp. cremoris LC330", J Dairy Res, 62, pp.621~628 (1995)[DOI]
- Looijesteijn PJ, "Uncoupling of growth and exopolysaccharide production by Lactococcus lactis subsp. cremoris NIZO B40 and optimization of its synthesis", J Biosci Bioeng, 88, pp.178~182 (1999)[DOI]
- "Codex stan 243-2003: codex standard for fermented milks. Codex Alimentarius" (2003)[URL]
- Pearse MJ, "Effect of casein micelle composition and casein dephosphorylation on coagulation and syneresis", J Dairy Res, 53, pp.381~390 (1986)[DOI]
- Kristo E, "The role of exopolysaccharide produced by Lactococcus lactis subsp. cremoris in structure formation and recovery of acid milk gels", Int Dairy J, 21, pp.656~652 (2011)[DOI]
- Yvon M, "An aminotransferase from Lactococcus lactis initiates conversion of amino acids to cheese flavor compounds", Appl Environ Microbiol, 63, pp.414~419 (1997)[DOI]
- Vinderola CG, "Survival of probiotic microflora in Argentinian yoghurts during refrigerated storage", Food Res Int, 33, pp.97~102 (2000)[DOI]