The Human Milk Oligosaccharide 2'-Fucosyllactose Shows an Immune-Enhancing Effect in a Cyclophosphamide-Induced Mouse Model
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The 2'-fucosyllactose (2'-FL) is the richest components in a human milk oligosaccharide. Several studies have reported that 2'-FL has beneficial effects in infants. However, there are few studies on its immune-enhancing effects. This research aimed to examine the immune-enhancing effect of 2'- FL on immunosuppression by cyclophosphamide (CCP) in ICR mice. Mice were orally administered distilled water or 0.5 mg/kg B.W. 2'-FL for 14 days. An immunocompromised mouse model was induced using CCP 80 mg/kg B.W. at 12-14 days. Using the CCP had effects on reducing their body weight, organ weight, spleen index, natural killer (NK) cell activity, and cytokines concentration and expression. This study also used concanavalin A-mediated T-cell proliferation to verify the immuneenhancing effects in the sample. Body weight, spleen index, organ weight, and cytokine levels were measured to estimate the immune-enhancing effects. The body weight at 14 days tended to increase, and the spleen weight and index significantly increased in the 2'-FL group compared to the CCP group. The NK cell activity increased in the 2'-FL group compared to the CCP group, but there was no significant difference. The concentration of interleukin (IL)-2 tended to recover in the 2'-FL group compared to the CCP group. The 2'-FL group showed a significant increase of IL-10 and IFNgamma concentration compared to the CCP group. In addition, there was a trend of increased IL-10 mRNA expression compared to the CCP group. These results revealed that 2'-FL improved CCPinduced immunosuppression, suggesting that 2'-FL may have the potential to enhance the immune system.
Abstract
The 2'-fucosyllactose (2'-FL) is the richest components in a human milk oligosaccharide. Several studies have reported that 2'-FL has beneficial effects in infants. However, there are few studies on its immune-enhancing effects. This research aimed to examine the immune-enhancing effect of 2'- FL on immunosuppression by cyclophosphamide (CCP) in ICR mice. Mice were orally administered distilled water or 0.5 mg/kg B.W. 2'-FL for 14 days. An immunocompromised mouse model was induced using CCP 80 mg/kg B.W. at 12-14 days. Using the CCP had effects on reducing their body weight, organ weight, spleen index, natural killer (NK) cell activity, and cytokines concentration and expression. This study also used concanavalin A-mediated T-cell proliferation to verify the immuneenhancing effects in the sample. Body weight, spleen index, organ weight, and cytokine levels were measured to estimate the immune-enhancing effects. The body weight at 14 days tended to increase, and the spleen weight and index significantly increased in the 2'-FL group compared to the CCP group. The NK cell activity increased in the 2'-FL group compared to the CCP group, but there was no significant difference. The concentration of interleukin (IL)-2 tended to recover in the 2'-FL group compared to the CCP group. The 2'-FL group showed a significant increase of IL-10 and IFNgamma concentration compared to the CCP group. In addition, there was a trend of increased IL-10 mRNA expression compared to the CCP group. These results revealed that 2'-FL improved CCPinduced immunosuppression, suggesting that 2'-FL may have the potential to enhance the immune system.
참고문헌 (45)
- Prentice AM, "Evolutionary and enviromental influences on human lactation", Proc. Nutr. Soc, 54, pp.391~400 (1995)
- Ladomenou F, "Protective effect of exclusive breastfeeding against infections during infancy: a prospective study", Arch. Dis. Child, 95, pp.1004~1008 (2010)
- Italianer MF, "Circadian variation in human milk composition, a systematic review", Nutrients, 12, pp.2328~ (2020)
- Casado B, "OMICS-rooted studies of milk proteins, oligosaccharides and lipids", J. Proteomics, 73, pp.196~208 (2009)
- Buescher ES, "Anti-inflammatory characteristics of human milk: how, where, why", Adv. Exp. Med. Biol, 501, pp.207~222 (2001)
- Kong C, "Impact of dietary fibers in infant formulas on gut microbiota and the intestinal immune barrier", Food Funct, 11, pp.9445~9467 (2020)
- Bode L, "Human milk oligosaccharides: every baby needs a sugar mama", Glycobiology, 22, pp.1147~1162 (2012)
- Smilowitz JT, "Breast milk oligosaccharides: structure-function relationships in the neonate", Annu. Rev. Nutr, 34, pp.143~169 (2014)
- Duska-McEwen G, "Human milk oligosaccharides enhance innate immunity to respiratory syncytial virus and influenza in vitro", Food Nutr. Sci, 14, pp.13~ (2014)
- Reverri EJ, "Review of the clinical experiences of feeding infants formula containing the human milk oligosaccharide 2′-fucosyllactose", Nutrients, 10, pp.1346~ (2018)
- Goehring KC, "Similar to those who are breastfed, infants fed a formula containing 2′-fucosyllactose have lower inflammatory cytokines in a randomized controlled trial", J. Nutr, 146, pp.2559~2566 (2016)
- Arnold H, "Chemotherapeutic action of a cyclic nitrogen mustard phosphamide ester (B 518-ASTA) in experimental tumours of the rat", Nature, 181, pp.931~ (1958)
- Shirani K, "Phytotrapy of cyclophosphamide-induced immunosuppression", Environ. Toxicol. Pharmacol, 39, pp.1262~1275 (2015)
- Pass GJ, "Role of hepatic cytochrome p450s in the pharmacokinetics and toxicity of cyclophosphamide: studies with the hepatic cytochrome p450 reductase null mouse", Cancer Res, 65, pp.4211~4217 (2005)
- Al-Nasser IA, "In vivo prevention of cyclophosphamide-induced Ca2+ dependent damage of rat heart and liver mitochondria by cyclosporin A", Comp. Biochem. Physiol. A Mol. Integr. Physiol, 121, pp.209~214 (1998)
- Kanneganti T-D, "Innate immune pathways in host defense", Mediators Inflammn, 2012, pp.708972~ (2012)
- 이하늘, "Combination of vegetable soup and glucan demonstrates synergistic effects on macrophage-mediated immune responses", Food Science and Biotechnology, 30(4), pp.583~588 (2021), 한국식품과학회[DOI]
- Germic N, "Regulation of the innate immune system by autophagy: neutrophils, eosinophils, mast cells, NK cells", Cell Death Differ, 26, pp.703~714 (2019)
- 임정식, "Synergistic effect of Korean red ginseng extract and GABA mixture on the IgE production in mice via Th1", Th2 cell balance, 2021, pp.30(12), 1571~1580[DOI]
- Donovan SM, "Human milk oligosaccharides influence neonatal mucosal and systemic immunity", Ann. Nutr. Metab, 69, pp.41~51 (2016)
- Vos AP, "Immune-modulatory effects and potential working mechanisms of orally applied nondigestible carbohydrates", Crit. Rev. Immunol, 27, pp.97~140 (2007)
- Chen LX, "A Comparative study on the effects of different parts of Panax ginseng on the immune activity of cyclophosphamide-induced immunosuppressed mice", Molecules, 24, pp.1096~ (2019)
- Xu X, "Effects of cyclophosphamide on immune system and gut microbiota in mice", Microbiol. Res, 171, pp.97~106 (2015)
- Lori A, "The Spleen: A Hub connecting nervous and immune systems in cardiovascular and metabolic diseases", Int. J. Mol. Sci, 18, pp.1216~ (2017)
- Raj S, "Immunomodulatory activity of methanolic extract of Amorphophallus commutatus var. wayanadensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models", Food Chem. Toxicol, 81, pp.151~159 (2015)
- Sarangi I, "Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans", Int. Immunopharmacol, 6, pp.1287~1297 (2006)
- Moretta A, "What is a natural killer cell?", Nat. Immunol, 3, pp.6~8 (2002)
- Lemire P, "Natural killer cell functions during the innate immune response to pathogenic Streptococci", Front. Microbiol, 8, pp.1196~ (2017)
- Adib-Conquy M, "TLR-mediated activation of NK cells and their role in bacterial", viral immune responses in mammals, 2014, pp.92, 256~262
- Souza-Fonseca-Guimaraes F, "Natural killer (NK) cells in antibacterial innate immunity:angels or devils?", Mol. Med, 18, pp.270~285 (2012)
- Stordeur P, "Immune monitoring in whole blood using real-time PCR", J. Immunol. Methods, 276, pp.69~77 (2003)
- Castano AP, "Photodynamic therapy plus low-dose cyclophosphamide generates antitumor immunity in a mouse model", Proc. Natl. Acad. Sci. USA, 105, pp.5495~5500 (2008)
- Hamodrakas SJ, "The crystal structure of the complex of concanavalin A with 4'-methylumbelliferyl-alpha-D-glucopyranoside", J. Struct. Biol, 118, pp.23~30 (1997)
- Lovatt M, "Different doses of agonistic ligand drive the maturation of functional CD4 and CD8 T cells from immature precursors", Eur. J. Immunol, 30, pp.371~381 (2000)
- Arango Duque G, "Macrophage cytokines: involvement in immunity and infectious diseases", Front. Immunol, 5, pp.491~ (2014)
- Kak G, "Interferon-gamma (IFN-γ): exploring its implications in infectious diseases", Biomol. Concepts, 9, pp.64~79 (2018)
- Azagra-Boronat I, "Supplementation with 2'-FL and scGOS", lcFOS ameliorates rotavirus-induced diarrhea in suckling fats, 2018, pp.8, 372~
- Azagra-Boronat I, "Immunomodulatory and prebiotic effects of 2'-fucosyllactose in suckling rats", Front. Immunol, 10, pp.1773~ (2019)
- He Y, "The human milk oligosaccharide 2′-fucosyllactose modulates CD14expression in human enterocytes, thereby attenuating LPS-induced inflammation", Gut, 65, pp.33~46 (2016)
- Mosmann TR, "The expanding universe of T-cell subsets: Th1, Th2 and more", Immunol. Today, 17, pp.138~146 (1996)
- Rothermel AL, "Differential abilities of Th1 and Th2 to induce polyclonal B cell proliferation", Cell. Immunol, 135, pp.1~15 (1991)
- Donovan SM, "Human milk oligosaccharides influence neonatal mucosal and systemic immunity", Ann. Nutr. Metab, 69(Suppl 2), pp.42~51 (2016)
- Yu ZT, "The human milk oligosaccharide 2'-fucosyllactose quenches campylobacter jejuniinduced inflammation in human epithelial cells HEp-2 and HT-29 and in mouse intestinal mucosa", J. Nutr, 146, pp.1980~1990 (2016)
- Li A, "The human milk oligosaccharide 2'-fucosyllactose attenuates β-lactoglobulininduced food allergy through the miR-146a-mediated toll-like receptor 4", nuclear factor-κB signaling pathway, 2021, pp.104, 10473~10484
- Chaiwat Monmai, "Anti-inflammatory activities of the mixture of strawberry and rice powder as materials of fermented rice cake on RAW264.7 macrophage cells and mouse models", Food Science and Biotechnology, 30(11), pp.1409~1416 (2021), 한국식품과학회[DOI]
