Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
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To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats. Methods: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then dividedinto three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. Aftereight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry,Immunofluorescence, and Western Blot about ezrin, merlin were done. Results: In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence,Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogeninjection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acidcomposition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expressionof ezrin and merlin. Conclusion: Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen dideffect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina. (J Menopausal Med 2014;20:97-103)
Abstract
To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats. Methods: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then dividedinto three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. Aftereight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry,Immunofluorescence, and Western Blot about ezrin, merlin were done. Results: In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence,Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogeninjection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acidcomposition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expressionof ezrin and merlin. Conclusion: Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen dideffect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina. (J Menopausal Med 2014;20:97-103)
참고문헌 (14)
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