본문 바로가기
KERD
논문KCI 등재

간세포성장인자가 하인두 편평세포암 세포주의 증식, 분산과 이동에 미치는 영향

Effect of HGF in Proliferation, Dispersion and Migration ofHypopharyngeal Squamous Cell Carcinoma

이재호|배준호|최은창|김철호|문성균
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2005.02|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제48권 제2호|pp. 208-215

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

간세포성장인자·c-Met·하인두암·증식·전이.Hepatocyte growth factor· c-Met· Hypopharyngeal cancer· Proliferation· Migration.
주제분류이비인후과학

초록

Abstract

Background and Objectives：Hepatocyte growth factor (HGF) is known to stimulate motility, invasivenes, proliferation, and morphogenesis of endothelial cells. Recent reports revealed that this growth factor is also related to tumor invasion and metas-tasis. We examined the role of HGF/c-Met on the proliferation, dispersion and migration of FaDu cell, a hypopharyngeal squa-mous cell carcinoma cell line. Materials and Method：We performed RT-PCR and Western blot in FaDu. Proliferation of the FaDu cels was assayed by counting the number of cells after treatment by HGF of diferent concentrations of 0, 10, 30 ng/mL. Dispersion of the cells was observed by measuring the separation and morphologic changes of cells after the colony of FaDu cells was formed in the media and then treated with HGF of 10 ng/mL or 30 ng/mL for 24 hours. Tumor cel migration was assesed by wound healing asay. Lastly, we examined the enhancement of HGF production in human fibroblast (MRC-5) by putative inducer secreted from FaDu cels. Results：popharyngeal cel line while that of HGF was not. Exogenous HGF significantly enhanced the growth of FaDu in a dose-dependent manner 30 ng/ml (p<0.05). HGF stimulated the dispersion and enhanced the migration of cancer cells in a dose-dependent manner (p<0.05). HGF produced by human stromal fibroblast (MRC-5) was increased by a certain inducer ori-ginated from FaDu cells (p<0.05). Conclusion：These results sugest that HGF may play an important role in the progresion of hypopharyngeal cancer through the enhancement of proliferation, dispersion and migration.

참고문헌 (23)

  1. "Tumour invasion:Effects of cell adhesion and motility", 2, pp.163~9 (1992)
  2. "Regulation of the cdk inhibitor p27 and its deregulation in cancer", 183, pp.10~7 (2000)
  3. "Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats", 122, pp.1450~9 (1984)
  4. "Scatter factor:Molecular characteristics and effect on the invasiveness of epithelial cells", 111, pp.2097~108 (1990)
  5. "Scatter factor induces blood vessel formation in vivo", 90, pp.1937~41 (1993)
  6. "Emerging multipotent aspects of hepatocyte growth factor", pp.591~600 (1996)
  7. "A multifunctional" (1995)
  8. "Creation of an hepatocyte growth factor", scatter factor autocrine loop in carcinoma cells induces invasive properties associated with increased tumorigenicity, 1994, pp.9, 1091~9
  9. "Significant correlation between serum level of hepatocyte growth factor and progression of gastric carcinoma", 23, pp.1176~80 (1999)
  10. "Role of HGF", c-met system in invasion and metastasis of oral squamous cell carcinoma cells in vitro and its clinical significance, 2001, pp.93, 489~96
  11. "mediated keratinocyte migration is coincident with induction of matrix metalloproteinase" (1998)
  12. "HGF", scatter factor selectively promotes cell invasion by increasing integrin avidity, 2000, pp.14, 1629~40
  13. "Regulation of scatter factor", hepatocyte growth factor responses by Ras and Rho in MDCK cells, 1995, ~
  14. "Effect of hepatocyte growth factor on assembly of zonula occludens-1 protein at the plasma membrane" (1998)
  15. "Activation of both MAP kinase and phosphatidylinositide 3-kinase by Ras is required for hepatocyte growth factor", scatter factor-induced adherens junction disassembly, 1998, pp.9, 2185~200
  16. "Tyrosine phosphorylation of beta-catenin and plakoglobin enhanced by hepatocyte growth factor and epidermal growth factor in human carcinoma cells", 1, pp.295~305 (1994)
  17. "Hepatocyte growth factor alters the polarity of Madin-Darby canine kidney cell monolayers", 272, pp.3471~7 (1997)
  18. "HGF", SF induces mesothelial cell migration and proliferation by autocrine and paracrine pathways, 2001, pp.267, 258~66
  19. "Enhanced modulation of keratinocyte motility by transforming growth factoralpha relative to epidermal growth factor", pp.590~7 (1996)
  20. "Autocrine activation of the hepatocyte growth factor receptor", met, 2002, ~
  21. "A study of an in vitro model for invasion of oral squamous cell carcinoma", 18, pp.498~501 (1989)
  22. "Regulation of fibroblast hepatocyte growth factor", scatter factor expression by human breast carcino-ma cell lines and peptide growth factors, 1993, pp.53, 1233~8
  23. "Induction of hepatocyte growth factor in fibroblasts by tumor-derived factors affects invasive growth of tumor cells In vitro analysis of tumor-stromal interactions", ta(y)

관련 논문AI추천

하인두 편평세포암에서 간세포성장인자와 그 수용체인 c-Met의 발현김철호, 문성균, 배준호, 이재호, 최은창, 한재호대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제11호구강 및 구인두 편평세포암종에서 Hepatocyte Growth Factor와c-met 유전자 발현의 의의김진환, 노영수, 신형식, 안회영, 오소정, 조성진대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2007 · 제50권 제8호암의 성장에 미치는 중간엽줄기세포의 효과박영대, 신성찬, 이병주대한이비인후과학회 부산,울산,경남 지부회 · 임상이비인후과2014 · 제25권 제2호후두편평세포암종에서 Vascular Endothelial Growth Factor의 발현김훈, 이강원, 이규석, 임도형, 홍영호대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제3호두경부 편평세포암종에서 전이와 예후 인자에 대한 연구김옥준, 서구종, 유선열, 최홍란대한구강악안면외과학회 · 대한구강악안면외과학회지2002 · 제28권 제1호하인두 편평세포암주의 증식과 침습을 유도하는 간세포성장인자에 대한 녹차추출물 억제효과강성운, 김동현, 김철호, 윤용로, 이하늘, 임혜진, 황혜숙대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2008 · 제51권 제2호

이 간행물의 다른 논문 (11건)

두경부 재건의 최신 지견：두경부 결손의 기능적 재건술김민식pp. 130-135외이도 편평상피암종의 치료 경험김한수, 김창우, 이원상, 정상호pp. 136-141소음성 난청에서 이명의 주파수와 작업장 소음 주파수의 관계조규섭, 이일우, 안정혁, 고의경, 전경명, 전준pp. 142-145돌발성 난청 환자에서 Lasix, 비타민, Carbogen 흡입 병합 요법의 치료 효과와 치료 시기에 따른 예후 인자의 분석김훈, 김경수, 김희종, 홍영호, 양훈식, 서강욱pp. 146-152아세틸콜린이 외유모세포 칼륨 전류 및 길이 변화에 미치는 영향황매, 조용범, 장명주, 이준규, 정한성, 조형호, 이재홍, 박종성pp. 153-157
원문 보기