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이종이식된 두경부영역의 편평세포암종에서9-Hydroxypheophorbide-α와 630 nm 다이오드 레이저를이용한 광역학치료의 항암효과 및 치료기전에 대한 연구

The Anticancer Effect and Mechanism of Photodynamic Therapy Using9-Hydroxypheophorbide-α and 630 nm Diode Laser onHeterotransplanted Squamous Cell Carcinoma of Head and Neck

이상훈|이정구|김세형|정필상|김한균
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2005.06|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제48권 제6호|pp. 765-770

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참고문헌

키워드

광역학치료·광감작제·편평세포암종Photodynamic therapy·Photosensitizers·Squamous cell carcinoma.
주제분류이비인후과학

초록

Abstract

Background and Objectives：The 9-Hydroxypheophorbide-α(9-HpbD-α) is a new photosensitizer, derived from a plant inwater. We conducted a series of experiments in vivo to evaluate the anticancer effect and mechanism of photodynamic therapyusing 9-HpbD-α and 630 nm diode laser on squamous cell carcinoma. Materials and Method：SNU-1041 cell line washeterotransplanted into the subcutaneous space of nude mouse. When the tumors grew up to 400 mm3, the animals were randomlyseperated into 4 groups：Group I (n=5) of the normal control group；Group II (n=10), which received interstitial injectionof 0.007 μg/mm3 of 9-HpbD-α；Group III (n=10), which received irradiation with 1.6 J/mm3 of light using diode laser；Group IV (n=10), which received interstitial injection of 0.007 μg/mm3 of 9-HpbD-α followed by irradiation with 1.6 J/mm3of light 6 hours after the injection. After photodynamic therapy (PDT), tumor tissue was harvested for histopathologic studyunder light microscopy and transmission electron microscope (TEM). Results：PDT group (Group IV) showed significantremission rate (70 %), compared to control group (p<0.05). The microscopic findings of the tumor section were characterizedby massive necrosis and some apoptotic cells among the normal cells. TEM showed different morphologic changes betweennecrotic and apoptotic cells. These findings were considered as the evidence of direct cytotoxicity of PDT using 9-HpbD-α and630 nm diode laser. Conclusion：The results suggest that therapy using PDT, 9-HpbD-α and diode laser shows an anticancereffect. Its therapeutic mechanism appears to be based on necrosis that is caused by direct cytotoxicity.

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