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두경부 편평상피암 세포주에서 9-Hydroxypheophorbide-a를이용한 광역학치료의 ROS 발현 양상과 세포사에 관한 연구

ROS Formation and the Mechanism of Photodynamic Therapy Using9-Hydroxypheophorbide-a on AMC-HN3 HumanHead and Neck Carcinoma Cell Line

이기일|박으뜸|안진철|정필상
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2006.12|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제49권 제12호|pp. 1194-1200

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키워드

광역학치료·활성화 산소기·9-Hydroxypheophorbide-a.Photodynamic therapy (PDT)·Reactive oxygen species (ROS)·9-Hydroxypheophorbide-a.
주제분류이비인후과학

초록

Abstract

Background and Objectives：During PDT, photosensitizer accumulates in the cell and irradiation forms ROS. ROS leads to activationof apoptoticpathway and cell death. Elevated intracellular calcium is known to play important role in apoptotic pathway.There are two type of ROS formation. The type of ROS formation differs in type of photosensitizers. We designed the experimentto define the relationship of ROS and cell death in PDT. Materials and Method：AMC-HN3 cells were cultured. Using aCaspACE assay kit, we measured caspases-3 activity after PDT. We also observed intra-cellular calcium concentrations usingconfocal microscopy (calcium green-1 stain) after PDT. To determine which type of reaction occursduring ROS formation, MTTassay was performed. Results：Confocal microscopy showed that ROS had formed at the site of photosensitizer formation afterPDT. After PDT, intracellular calcium increased. MTT assay showed more viability increase in blocking type II reaction. Caspaseassay showed highest level after 4hrs. Conclusion：ROS is formed at the site photosentizer formation after PDT. Type II reactionwas the main type of ROS formation. Apoptosis was main pathway of cell death in low dose of photosensitizer after PDT. (KoreanJ Otolaryngol 2006;49:1194-200)

참고문헌 (15)

  1. "Mechanism of photodynamic therapy inducedcell death", 319, pp.342~358 (2000)
  2. "The photobiology of photodynamic therapy:Cellular targets and mechanisms.", 150, pp.146~156 (1998)
  3. "An update on photodynamic therapy applications", 20(1), pp.3~7 (2002)
  4. "Definition of type I and type II photosensitized oxidation"
  5. "The role of subcellular localizationin initiation of apoptosis by photodynamic therapy", 65(3), pp.422~426 (1997)
  6. "Differential cell death response tophotodynamic therapy is dependent on dose and cell type", 84(10), pp.1384~1386 (2001)
  7. "Anticancer effect and mechanism of photodynamic therapyusing 9-hydroxypheophorbide-a and 660 nm diode laser on humansquamous carcinoma cell line" (2003), Dankook Univ
  8. "Mitochondria and apoptosis", 281(5381), pp.1309~1312 (1998)
  9. "Mitochondria: Releasing powerfor life and unleashing the machineries of death.", 112(4), pp.481~490 (2003)
  10. "Calcium signaling and apoptosis", 304(3), pp.445~454 (2003)
  11. "Regulation of cell death: Thecalcium-apoptosis link", 4(7), pp.552~565 (2003)
  12. "Endoplasmic reticulum, Bcl-2 and Ca2C handling in apoptosis", 32, pp.413~420 (2002)
  13. "Changesin intracellular Ca2C concentrations related to PDT-induced apoptosisin photo-sensitized human cancer cells", 128(2), pp.205~210 (1998)
  14. "Calcium and oxidative stress: From cell signalingto cell death", 38(10), pp.713~721 (2001)
  15. "Evaluation of a soluble tetrazolium", formazan assay for cell growth anddrug sensitivity in culture using human and other tumor cell lines, 4827~33

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