Protective Effect of Metformin on Gentamicin-Induced Vestibulotoxicity in Rat Primary Cell Culture
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Objectives. One of the antidiabetic drugs, metformin, have shown that it prevented oxidative stress-induced death in sever- al cell types through a mechanism involving the opening of the permeability transition pore and cytochrome c re- lease. Thus, it is possible that the antioxidative effect of metformin can also serve as protection against gentamicin-in- duced cytotoxicity related to reactive oxygen species (ROS). The aim of this study was to examine the protective ef- fect of metformin on gentamicin-induced vestibulotoxicity in primary cell culture derived from rat utricle. Methods. For vestibular primary cell culture, rat utricles were dissected and incubated. Gentamicin-induced cytotoxicity was measured in both the auditory and vestibular cells. To examine the effects of metformin on gentamicin-induced cytotoxicity in the primary cell culture, the cells were pretreated with metformin at a concentration of 1 mM for 24 hours, and then exposed to 2.5 mM gentamicin for 48 hours. The intracellular ROS level was measured using a fluo- rescent dye, and also measured using a FACScan flow cytometer. Intracellular calcium levels in the vestibular cells were measured with calcium imaging using Fura-2 AM. Results. Vestibular cells were more sensitive to gentamicin-induced cytotoxicity than auditory hair cells. Metformin protects against gentamicin-induced cytotoxicity in vestibular cells. Metformin significantly reduced a gentamicin-induced in- crease in ROS, and also reduced an increase in intracellular calcium concentrations in gentamicin-induced cytotoxicity. Conclusion. Metformin significantly reduced a gentamicin-induced increase in ROS, stabilized the intracellular calcium concentration, and inhibited gentamicin-induced apoptosis. Thus, Metformin showed protective effect on gentamicin- induced cytotoxicity in vestibular primary cell culture.
Abstract
Objectives. One of the antidiabetic drugs, metformin, have shown that it prevented oxidative stress-induced death in sever- al cell types through a mechanism involving the opening of the permeability transition pore and cytochrome c re- lease. Thus, it is possible that the antioxidative effect of metformin can also serve as protection against gentamicin-in- duced cytotoxicity related to reactive oxygen species (ROS). The aim of this study was to examine the protective ef- fect of metformin on gentamicin-induced vestibulotoxicity in primary cell culture derived from rat utricle. Methods. For vestibular primary cell culture, rat utricles were dissected and incubated. Gentamicin-induced cytotoxicity was measured in both the auditory and vestibular cells. To examine the effects of metformin on gentamicin-induced cytotoxicity in the primary cell culture, the cells were pretreated with metformin at a concentration of 1 mM for 24 hours, and then exposed to 2.5 mM gentamicin for 48 hours. The intracellular ROS level was measured using a fluo- rescent dye, and also measured using a FACScan flow cytometer. Intracellular calcium levels in the vestibular cells were measured with calcium imaging using Fura-2 AM. Results. Vestibular cells were more sensitive to gentamicin-induced cytotoxicity than auditory hair cells. Metformin protects against gentamicin-induced cytotoxicity in vestibular cells. Metformin significantly reduced a gentamicin-induced in- crease in ROS, and also reduced an increase in intracellular calcium concentrations in gentamicin-induced cytotoxicity. Conclusion. Metformin significantly reduced a gentamicin-induced increase in ROS, stabilized the intracellular calcium concentration, and inhibited gentamicin-induced apoptosis. Thus, Metformin showed protective effect on gentamicin- induced cytotoxicity in vestibular primary cell culture.
참고문헌 (30)
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