비인강암에서 c-kit 단백의 발현 및 유전자 변이의 임상적 의미
Clinical Significance of c-kit Protein Expression andc-kit Gene Mutation in Nasopharyngeal Cancer
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Abstract
Background and Objectives:The proto-oncogene c-kit is a receptor tyrosine kinase recognized to initiate esential signal tra-nsduction pathways that transmit biological signals for cellular proliferation, diferentiation and metastasis. Aberrant expression -ing aditional groups of tumors that may use the stem cell factor/c-kit pathway, we investigated the expresion of c-kit in nasoph-aryngeal carcinoma. Subjects and Method:In this retrospective study, imunohistochemical stains for c-kit were performed on for-malin-fixed parafin embedded sections from 20 patients with nasopharyngeal carcinoma who were treated from 1996 to 2004. Gene mutation was analyzed by PCR-SSCP and direct sequencing. Results:c-kit over-expression was found in 65% (13/20) of patients. Eight of the 13 samples (61.5%) exhibited strongly positive immunoreactivity for c-kit protein (staining of >50% of the tumor cells). c-kit gene mutation was found in 4 of 20 cases by the PCR-SSCP method. Conclusion:c-kit protein over-expre-sion is found in 65% of the nasopharyngeal carcinoma patients. c-kit expression is corelated with c-kit DNA mutations and naso-pharyngeal carcinoma may be potential targets for treatment with imatinib mesylate. (Korean J Otolaryngol 207 ;50 :519-24)
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