알레르기성 비염 환자에서의 비강유발검사 후 비즙내 알부민을 이용한 비즙 채취 방법의 비교
Comparison of Collecting Methods of Nasal Fluids Using Albumin after Nasal Provocation in Allergic Patients
0
조회
0
다운로드
1
인용
0
참고문헌
초록
Background and Objectives Although different methods of collecting nasal fluid exist and can influence the result of the study, there have not been any studies made yet to compare the different methods. The authors aimed to evaluate the usefulness of these methods by comparing the albumin concentration of nasal fluid after nasal provocation in patients with allergic rhinitis. Subjects and Method In 22 patients with allergic rhinitis, we measured the change of nasal obstruction and rhinorrhea after nasal provocation using Visual Analogue Scale (VAS). In Group A (n=10), the nasal lavage and direct suction method were used and in Group B (n=12), nasal lavage and filter paper method were used. Nasal fluids were obtained before, 20 min after and 8 hours after provocation. The concentration of albumin was calculated using rate nephelometry. Results Patientsʼ nasal symptoms were aggravated in early allergic reaction but subsided in late reaction. There was meaningful increase of albumin concentration with the use of direct suction and filter paper methods. In contrast, there was no significant increase of concentration with the use of lavage method. Conclusion The filter paper method is superior in the quantitative analysis of mediators in nasal fluid after nasal provocation in allergic patients. The lavage method, however, is considered inappropriate because of its dilution effect. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:615-9
Abstract
Background and Objectives Although different methods of collecting nasal fluid exist and can influence the result of the study, there have not been any studies made yet to compare the different methods. The authors aimed to evaluate the usefulness of these methods by comparing the albumin concentration of nasal fluid after nasal provocation in patients with allergic rhinitis. Subjects and Method In 22 patients with allergic rhinitis, we measured the change of nasal obstruction and rhinorrhea after nasal provocation using Visual Analogue Scale (VAS). In Group A (n=10), the nasal lavage and direct suction method were used and in Group B (n=12), nasal lavage and filter paper method were used. Nasal fluids were obtained before, 20 min after and 8 hours after provocation. The concentration of albumin was calculated using rate nephelometry. Results Patientsʼ nasal symptoms were aggravated in early allergic reaction but subsided in late reaction. There was meaningful increase of albumin concentration with the use of direct suction and filter paper methods. In contrast, there was no significant increase of concentration with the use of lavage method. Conclusion The filter paper method is superior in the quantitative analysis of mediators in nasal fluid after nasal provocation in allergic patients. The lavage method, however, is considered inappropriate because of its dilution effect. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:615-9
참고문헌 (15)
- Watelet JB, "Collection of nasal secretions for immunological analysis", Eur Arch Otorhinolaryngol, 261(5), pp.242~246 (2004)
- Klimek L, "Norm values for eosinophil cationic protein in nasal secretions: influence of specimen collection", Clin Exp Allergy, 29(3), pp.367~374 (1999)
- Maremmani C, "Electrophoretic pattern of physiological human nasal secretions", Rhinology, 34(3), pp.147~150 (1996)
- Hilding AC, "Simple method for collecting near-normal human nasal secretion", Ann Otol Rhinol Laryngol, 81(3), pp.422~423 (1972)
- Biewenga J, "Nasal secretions from patients with polyps and healthy individuals, collected with a new aspiration system: evaluation of total protein and immunoglobulin concentrations", Ann Clin Biochem, 28(3), pp.260~266 (1991)
- Adkinson NF Jr, "Middleton’s allergy: principles and practice. 7th ed", pp.1281~1282 (2009), Mosby
- Wang DY, "Pathogenic mechanisms underlying the clinical symptoms of allergic rhinitis", Am J Rhinol, 14(5), pp.325~333 (2000)
- Yamazaki M, "Use of a rapid detection assay for influenza virus, on nasal aspirate specimens", Kansenshogaku Zasshi, 73, pp.1064~1068 (1999)
- Besançon-Watelet C, "Eosinophilia and cell activation mediators in nasal secretions", Laryngoscope, 112(1), pp.43~46 (2002)
- Niehaus MD, "Lactoferrin and eosinophilic cationic protein in nasal secretions of patients with experimental rhinovirus colds, natural colds, and presumed acute community-acquired bacterial sinusitis", J Clin Microbiol, 38(8), pp.3100~3102 (2000)
- Kim SS, "Levels of intracellular protein and messenger RNA of mucin and lysozyme in normal human nasal and polyp epithelium", Laryngoscope, 110, pp.276~280 (2000)
- Ingels K, "Nasal biopsy is superior to nasal smear for finding eosinophils in nonallergic rhinitis", Allergy, 52(3), pp.338~341 (1997)
- Gevaert P, "Nasal IL-5 levels determine the response to anti-IL-5 treatment in patients with nasal polyps", J Allergy Clin Immunol, 118(5), pp.1133~1141 (2006)
- Klimek L, "Methods for monitoring of therapeutic efficacy in immunotherapy of allergic rhinitis", Wien Med Wochenschr, 149(15), pp.394~402 (1999)
- Pupek M, "Time-dependent observations of secretion marker levels in nasal secretion after histamine and methacholine provocations", Arch Immunol Ther Exp, 51(4), pp.259~265 (2003)