Interleukin-10 발현 B 세포가 만성 비부비동염의 병태생리에 미치는 영향
Role of Interleukin-10-Expressing B Cells on the Pathophysiology of Chronic Sinusitis
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Background and Objectives Many kinds of inflammatory cells and cytokines are suggestedto be related with pathophysiology of chronic rhinosinusitis with nasal polyp (CRSwNP),but its mechanism is not yet fully understood. In particular, little is known about the functionand role of Interleukin-10 (IL-10) producing regulatory B cells. The aim of this study was toinvestigate the presence and function of B cells, especially IL-10-producing regulatory Bcells, in the nasal polyp (NP) and nasal mucosa. Subjects and Method Twenty patients with CRSwNP, 10 patients with chronic rhinosinusitiswithout nasal polyp (CRSsNP) and 10 control subjects were enrolled in this study. NP tissuesand uncinate tissues were collected for analysis. Real-time polymerase chain reaction (RTPCR),immunohistochemistry were performed to measure the expression levels of the selectedinflammatory cytokines and inflammation-associated molecules. Results In the mucosal tissues of CRSsNP patients, the number of IL-10+ B cells was significantlylower than that of NP and control mucosa of CRSwNP patients. The number of IL-10+B cells was significantly increased in the eosinophilic NP, non-eosinohilic NP, CRSsNP, andcontrol groups. There was a significant positive correlation between the number of IL-10+ Bcells and B cell activating factor (BAFF). Conclusion The expression of IL-10+ B cells and BAFF was significantly increased inCRSwNP patients compared to CRSsNP patients and control mucosal tissues, and IL-10+ Bcells were more significantly increased in eosinophilic tissues. These results indicate that regulatoryB cells are involved in the pathophysiology of eosinophilic NP and that BAFF contributesto the production of regulatory B cells.
Abstract
Background and Objectives Many kinds of inflammatory cells and cytokines are suggestedto be related with pathophysiology of chronic rhinosinusitis with nasal polyp (CRSwNP),but its mechanism is not yet fully understood. In particular, little is known about the functionand role of Interleukin-10 (IL-10) producing regulatory B cells. The aim of this study was toinvestigate the presence and function of B cells, especially IL-10-producing regulatory Bcells, in the nasal polyp (NP) and nasal mucosa. Subjects and Method Twenty patients with CRSwNP, 10 patients with chronic rhinosinusitiswithout nasal polyp (CRSsNP) and 10 control subjects were enrolled in this study. NP tissuesand uncinate tissues were collected for analysis. Real-time polymerase chain reaction (RTPCR),immunohistochemistry were performed to measure the expression levels of the selectedinflammatory cytokines and inflammation-associated molecules. Results In the mucosal tissues of CRSsNP patients, the number of IL-10+ B cells was significantlylower than that of NP and control mucosa of CRSwNP patients. The number of IL-10+B cells was significantly increased in the eosinophilic NP, non-eosinohilic NP, CRSsNP, andcontrol groups. There was a significant positive correlation between the number of IL-10+ Bcells and B cell activating factor (BAFF). Conclusion The expression of IL-10+ B cells and BAFF was significantly increased inCRSwNP patients compared to CRSsNP patients and control mucosal tissues, and IL-10+ Bcells were more significantly increased in eosinophilic tissues. These results indicate that regulatoryB cells are involved in the pathophysiology of eosinophilic NP and that BAFF contributesto the production of regulatory B cells.
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